Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-10

AUTHORS

Giampietro G. Schiavo, Fabio Benfenati, Bernard Poulain, Ornella Rossetto, Patrizia Polverino de Laureto, Bibhuti R. DasGupta, Cesare Montecucco

ABSTRACT

CLOSTRIDIAL neurotoxins, including tetanus toxin and the seven serotypes of botulinum toxin (A–G), are produced as single chains and cleaved to generate toxins with two chains joined by a single disulphide bond (Fig. 1). The heavy chain (Mr 100,000 (100K)) is responsible for specific binding to neuronal cells and cell penetration of the light chain (50K), which blocks neurotransmitter release1–9. Several lines of evidence have recently suggested that clostridial neurotoxins could be zinc endopeptidases2,10–14. Here we show that tetanus and botulinum toxins serotype B are zinc endopeptidases, the activation of which requires reduction of the interchain disulphide bond. The protease activity is localized on the light chain and is specific for synaptobrevin, an integral membrane protein of small synaptic vesicles. The rat synaptobrevin-2 isoform is cleaved by both neurotoxins at the same single site, the peptide bond Gln76-Phe77, but the isoform synaptobrevin-1, which has a valine at the corresponding position, is not cleaved. The blocking of neurotransmitter release of Aplysia neurons injected with tetanus toxin or botulinum toxin serotype B is substantially delayed by peptides containing the synaptobrevin-2 cleavage site. These results indicate that tetanus and botulinum B neurotoxins block neurotransmitter release by cleaving synaptobrevin-2, a protein that, on the basis of our results, seems to play a key part in neurotransmitter release. More... »

PAGES

832-835

Journal

TITLE

Nature

ISSUE

6398

VOLUME

359

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/359832a0

    DOI

    http://dx.doi.org/10.1038/359832a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1005284215

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1331807


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