Raf-1 activates MAP kinase-kinase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-07

AUTHORS

John M. Kyriakis, Harald App, Xian-feng Zhang, Papia Banerjee, David L. Brautigan, Ulf R. Rapp, Joseph Avruch

ABSTRACT

THE normal cellular homologue of the acutely transforming oncogene v-ra/is c-raf-l, which encodes a serine/threonine protein kinase that is activated by many extracellular stimuli1. The physiological substrates of the protein c-Raf-1 are unknown. The mitogen-activated protein (MAP) kinases ErkI and 2 are also activated by mitogens through phosphorylation of Erk tyrosine and threonine residues catalysed by a protein kinase of relative molecular mass 50,000, MAP kinase-kinase (MAPK-K)2–7. Here we report that MAPK-K as well as Erkl and 2 are constitutively active in v-raf-transformed cells. MAPK-K partially purified from v-raf-transformed cells or from mitogen-treated cells3 can be deactivated by phosphatase 2A. c-Raf-1 purified after mitogen stimulation can reactivate the phosphatase 2A-inactivated MAPK-K over 30-fold in vitro. c-Raf-1 reactivation of MAPK-K coincides with the selective phosphorylation at serine/threonine residues of a polypeptide with Mr 50,000 which coelutes precisely on cation-exchange chromatography with the MAPK-K activatable by c-Raf-1. These results indicate that c-Raf-1 is an immediate upstream activator of MAPK-K in vivo. To our knowledge, MAPK-K is the first physiological substrate of the c-raf-l protooncogene product to be identified. More... »

PAGES

417-421

Journal

TITLE

Nature

ISSUE

6385

VOLUME

358

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/358417a0

    DOI

    http://dx.doi.org/10.1038/358417a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000021328

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1322500


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