Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-03

AUTHORS

Robert H. Chow, Ludolf von Rüden, Erwin Neher

ABSTRACT

I N synapses, a rise in presynaptic intracellular calcium leads to secretory vesicle fusion in less than a millisecond, as indicated by the short delay from excitation to postsynaptic signal1–4. In non-synaptic secretory cells, studies at high time resolution have been limited by the lack of a detector as fast and sensitive as the postsynaptic membrane. Electrochemical methods may be sensitive enough to detect catecholamines released from single vesicles5,6. Here, we show that under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector. These signals obey statistics characteristic for quantal release; however, in contrast to neuronal transmitter release, secretion occurs with a significant delay after short step depolarizations. Furthermore, we identify a pedestal or 'foot' at the onset of unitary events which may represent the slow leak of catecholamine molecules out of a narrow 'fusion pore' before the pore dilates for complete exocytosis. More... »

PAGES

60-63

References to SciGraph publications

  • 1981-08. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1988-02. Patch-clamp techniques for time-resolved capacitance measurements in single cells in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1986-02. Neurotransmitter release and its facilitation in crayfish in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/356060a0

    DOI

    http://dx.doi.org/10.1038/356060a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1040050408

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1538782


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