Ontology type: schema:ScholarlyArticle
1992-03
AUTHORSRobert H. Chow, Ludolf von Rüden, Erwin Neher
ABSTRACTI N synapses, a rise in presynaptic intracellular calcium leads to secretory vesicle fusion in less than a millisecond, as indicated by the short delay from excitation to postsynaptic signal1–4. In non-synaptic secretory cells, studies at high time resolution have been limited by the lack of a detector as fast and sensitive as the postsynaptic membrane. Electrochemical methods may be sensitive enough to detect catecholamines released from single vesicles5,6. Here, we show that under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector. These signals obey statistics characteristic for quantal release; however, in contrast to neuronal transmitter release, secretion occurs with a significant delay after short step depolarizations. Furthermore, we identify a pedestal or 'foot' at the onset of unitary events which may represent the slow leak of catecholamine molecules out of a narrow 'fusion pore' before the pore dilates for complete exocytosis. More... »
PAGES60-63
http://scigraph.springernature.com/pub.10.1038/356060a0
DOIhttp://dx.doi.org/10.1038/356060a0
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/1538782
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