Selection of single-stranded DNA molecules that bind and inhibit human thrombin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-02

AUTHORS

L C Bock, L C Griffin, J A Latham, E H Vermaas, J J Toole

ABSTRACT

Aptamers are double-stranded DNA or single-stranded RNA molecules that bind specific molecular targets. Large randomly generated populations can be enriched in aptamers by in vitro selection and polymerase chain reaction. But so far single-stranded DNA has not been investigated for aptamer properties, nor has a target protein been considered that does not interact physiologically with nucleic acid. Here we describe the isolation of single-stranded DNA aptamers to the protease thrombin of the blood coagulation cascade and report binding affinities in the range 25-200 nM. Sequence data from 32 thrombin aptamers, selected from a pool of DNA containing 60 nucleotides of random sequence, displayed a highly conserved 14-17-base region. Several of these aptamers at nanomolar concentrations inhibited thrombin-catalysed fibrin-clot formation in vitro using either purified fibrinogen or human plasma. More... »

PAGES

564-566

Journal

TITLE

Nature

ISSUE

6360

VOLUME

355

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/355564a0

    DOI

    http://dx.doi.org/10.1038/355564a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000063370

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1741036


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    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/355564a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/355564a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/355564a0'

    RDF/XML is a standard XML format for linked data.

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