A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-01

AUTHORS

A Finkelstein, C F Kostrub, J Li, D P Chavez, B Q Wang, S M Fang, J Greenblatt, Z F Burton

ABSTRACT

RAP30/74 (also known as TFIIF, beta gamma and FC is one of several general factors required for initiation by RNA polymerase II. The small RAP30 subunit of RAP30/74 binds directly to polymerase and appears structurally and functionally homologous to bacterial sigma factors in their RNA polymerase-binding region. RAP30/74 or recombinant RAP30 suppresses nonspecific binding of RNA polymerase II to DNA and is required for RNA polymerase II to assemble stably into a preinitiation complex containing promoter DNA and the general factors TFIID, TFIIA and TFIIB; both RAP30 and RAP74 are physical components of the preinitiation complex. A complementary DNA encoding human RAP30 has been isolated, and here we report the isolation of a cDNA encoding human RAP74. RAP30 and RAP74 produced in Escherichia coli can be used in place of natural human RAP30/74 to direct accurate transcription initiation by RNA polymerase II in vitro. More... »

PAGES

464-467

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/355464a0

DOI

http://dx.doi.org/10.1038/355464a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046308434

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1734284


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