Normal development and function of CD8+ cells but markedly decreased helper cell activity in mice lacking CD4 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09-12

AUTHORS

A. Rahemtulla, W. P. Fung-Leung, M. W. Schilham, T. M. Kündig, S. R. Sambhara, A. Narendran, A. Arabian, A. Wakeham, C. J. Paige, R. M. Zinkernagel, R. G. Miller, T. W. Mak

ABSTRACT

T CELLS express T-cell antigen receptors (TCR) for the recognition of antigen in conjunction with the products of the major histocompatibility complex1,2. They also express two key surface coreceptors, CD4 and CDS, which are involved in the interaction with their ligands3,4. As CD4 is expressed on the early haemopoietic progenitor5 as well as the early thymic precursor cells6, a role for CD4 in haemopoiesis and T-cell development is implicated. Thymocytes undergo a series of differentiation7 and selection steps8,9 to become mature CD4+8− or CD4−8+ (single positive) T cells10,11. Studies of the role of CD4+ T cells in vivo have been based on adoptive transfer of selected or depleted lymphocytes, or in vivo treatment of thymectomized mice with monoclonal antibodies causing depletion of CD4+ T cells12–14. In order to study the role of the CD4 molecule in the development and function of lymphocytes, we have disrupted the CD4 gene in embryonic stem cells15–16 by homologous recombination17–18. Germ-line transmission19–20 of the mutation produces mutant mouse strains that do not express CD4 on the cell surface. In these mice, the development of CD8+ T cells and myeloid components is unaltered, indicating that expression of CD4 on progenitor cells and CD4+ CD8+ (double positive) thymocytes is not obligatory. Here we report that these mice have markedly decreased helper cell activity for antibody responses, although cytotoxic T-cell activity against viruses is in the normal range. This differential requirement for CD4+ helper T cells is important to our understanding of immune disorders, including AIDS, in which CD4+ cells are reduced or absent. More... »

PAGES

180-184

Journal

TITLE

Nature

ISSUE

6340

VOLUME

353

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/353180a0

DOI

http://dx.doi.org/10.1038/353180a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037128186

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1832488


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibody Formation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD4 Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytotoxicity, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Mutational Analysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Interleukin-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymph Nodes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphocyte Culture Test, Mixed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Mutant Strains", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spleen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocyte Subsets", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Cytotoxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Helper-Inducer", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thymus Gland", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rahemtulla", 
        "givenName": "A.", 
        "id": "sg:person.0746777450.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746777450.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fung-Leung", 
        "givenName": "W. P.", 
        "id": "sg:person.0632551050.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632551050.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schilham", 
        "givenName": "M. W.", 
        "id": "sg:person.01013477301.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013477301.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "K\u00fcndig", 
        "givenName": "T. M.", 
        "id": "sg:person.0751315141.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751315141.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sambhara", 
        "givenName": "S. R.", 
        "id": "sg:person.01003673072.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003673072.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Narendran", 
        "givenName": "A.", 
        "id": "sg:person.0644157012.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644157012.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Arabian", 
        "givenName": "A.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wakeham", 
        "givenName": "A.", 
        "id": "sg:person.0677274536.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677274536.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Paige", 
        "givenName": "C. J.", 
        "id": "sg:person.01137533300.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137533300.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zinkernagel", 
        "givenName": "R. M.", 
        "id": "sg:person.01135675570.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135675570.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "R. G.", 
        "id": "sg:person.01120655377.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120655377.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada", 
          "id": "http://www.grid.ac/institutes/grid.17063.33", 
          "name": [
            "Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mak", 
        "givenName": "T. W.", 
        "id": "sg:person.014440054532.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014440054532.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/328077a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005872580", 
          "https://doi.org/10.1038/328077a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/349071a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000779593", 
          "https://doi.org/10.1038/349071a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/308149a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050508510", 
          "https://doi.org/10.1038/308149a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/292154a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017487986", 
          "https://doi.org/10.1038/292154a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/312548a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003114730", 
          "https://doi.org/10.1038/312548a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/336684a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038677733", 
          "https://doi.org/10.1038/336684a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/276829a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015371060", 
          "https://doi.org/10.1038/276829a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/338153a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004411576", 
          "https://doi.org/10.1038/338153a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/317230a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005647127", 
          "https://doi.org/10.1038/317230a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/333742a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018116885", 
          "https://doi.org/10.1038/333742a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/308145a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021425337", 
          "https://doi.org/10.1038/308145a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/323445a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047908913", 
          "https://doi.org/10.1038/323445a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1991-09-12", 
    "datePublishedReg": "1991-09-12", 
    "description": "T CELLS express T-cell antigen receptors (TCR) for the recognition of antigen in conjunction with the products of the major histocompatibility complex1,2. They also express two key surface coreceptors, CD4 and CDS, which are involved in the interaction with their ligands3,4. As CD4 is expressed on the early haemopoietic progenitor5 as well as the early thymic precursor cells6, a role for CD4 in haemopoiesis and T-cell development is implicated. Thymocytes undergo a series of differentiation7 and selection steps8,9 to become mature CD4+8\u2212 or CD4\u22128+ (single positive) T cells10,11. Studies of the role of CD4+ T cells in vivo have been based on adoptive transfer of selected or depleted lymphocytes, or in vivo treatment of thymectomized mice with monoclonal antibodies causing depletion of CD4+ T cells12\u201314. In order to study the role of the CD4 molecule in the development and function of lymphocytes, we have disrupted the CD4 gene in embryonic stem cells15\u201316 by homologous recombination17\u201318. Germ-line transmission19\u201320 of the mutation produces mutant mouse strains that do not express CD4 on the cell surface. In these mice, the development of CD8+ T cells and myeloid components is unaltered, indicating that expression of CD4 on progenitor cells and CD4+ CD8+ (double positive) thymocytes is not obligatory. Here we report that these mice have markedly decreased helper cell activity for antibody responses, although cytotoxic T-cell activity against viruses is in the normal range. This differential requirement for CD4+ helper T cells is important to our understanding of immune disorders, including AIDS, in which CD4+ cells are reduced or absent.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/353180a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6340", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "353"
      }
    ], 
    "keywords": [
      "helper cell activity", 
      "T cells", 
      "cell activity", 
      "development of CD8", 
      "depletion of CD4", 
      "role of CD4", 
      "function of CD8", 
      "T cell activity", 
      "expression of CD4", 
      "helper T cells", 
      "function of lymphocytes", 
      "recognition of antigen", 
      "T cell development", 
      "T cell antigen receptor", 
      "myeloid component", 
      "adoptive transfer", 
      "antibody response", 
      "immune disorders", 
      "CD4", 
      "mature CD4", 
      "vivo treatment", 
      "major histocompatibility", 
      "mutant mouse strains", 
      "normal range", 
      "CD4 molecule", 
      "CD8", 
      "mouse strains", 
      "monoclonal antibodies", 
      "antigen receptor", 
      "mice", 
      "progenitor cells", 
      "lymphocytes", 
      "CD4 gene", 
      "thymocytes", 
      "cells", 
      "differential requirement", 
      "normal development", 
      "cell surface", 
      "antigen", 
      "AIDS", 
      "histocompatibility", 
      "coreceptor", 
      "activity", 
      "antibodies", 
      "receptors", 
      "disorders", 
      "role", 
      "virus", 
      "treatment", 
      "haemopoiesis", 
      "vivo", 
      "cells6", 
      "expression", 
      "development", 
      "mutations", 
      "response", 
      "depletion", 
      "function", 
      "study", 
      "genes", 
      "strains", 
      "conjunction", 
      "series", 
      "recognition", 
      "understanding", 
      "molecules", 
      "components", 
      "selection", 
      "interaction", 
      "CDS", 
      "products", 
      "range", 
      "transfer", 
      "order", 
      "requirements", 
      "surface"
    ], 
    "name": "Normal development and function of CD8+ cells but markedly decreased helper cell activity in mice lacking CD4", 
    "pagination": "180-184", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037128186"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/353180a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1832488"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/353180a0", 
      "https://app.dimensions.ai/details/publication/pub.1037128186"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-11-24T20:47", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_241.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/353180a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/353180a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/353180a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/353180a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/353180a0'


 

This table displays all metadata directly associated to this object as RDF triples.

345 TRIPLES      21 PREDICATES      134 URIs      114 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/353180a0 schema:about N114c729ca2ed415eb004c0fd967ec0f5
2 N158950050b274e8b91071f5b527fc2b6
3 N22a493e916574eab8ba779ec4f396b04
4 N29fc4a1b267d47c2a72032e43cf68819
5 N44bd8d0ef83b451d86628d2d2fbc2c48
6 N493dd507787d40e386d47703e95d2e2d
7 N8d43e25731184913b87cb3efde7d4ed9
8 Na32bc5992ed0453ebcfdd4dac48ac9dd
9 Na59a8c8a9a074540a68c627d808940f5
10 Nb9d71d0f946f4e1eb09dcfa078d83c3b
11 Nbf8fe3f39331486e91d47a0357aa9ee8
12 Nc595e2f0f66445c6b2258aa1bb6eefe4
13 Nc6107f180cfa402bab3a18116c5f0cc2
14 Nc899ae02504e4e17be3e4cc08e83fee5
15 Ne2152242a2dd433689ea63864c3a6a51
16 Ne23d39bfb56a4eedb9ebed0d986ada6f
17 Nea78fd3af6f549c499e39d1920ffbe3f
18 Neac1f9da1e884c33af330eea12138d9c
19 Nf9b3cb0cf8d846ef96c9efa45a248595
20 Nfae2ea70605940b38606b2ad09e357e1
21 Nfcdd288e1d114017ae95d92c8ba3ca68
22 anzsrc-for:11
23 anzsrc-for:1107
24 schema:author Nc3b00b7e975042b5a260cd7235d0a64a
25 schema:citation sg:pub.10.1038/276829a0
26 sg:pub.10.1038/292154a0
27 sg:pub.10.1038/308145a0
28 sg:pub.10.1038/308149a0
29 sg:pub.10.1038/312548a0
30 sg:pub.10.1038/317230a0
31 sg:pub.10.1038/323445a0
32 sg:pub.10.1038/328077a0
33 sg:pub.10.1038/333742a0
34 sg:pub.10.1038/336684a0
35 sg:pub.10.1038/338153a0
36 sg:pub.10.1038/349071a0
37 schema:datePublished 1991-09-12
38 schema:datePublishedReg 1991-09-12
39 schema:description T CELLS express T-cell antigen receptors (TCR) for the recognition of antigen in conjunction with the products of the major histocompatibility complex1,2. They also express two key surface coreceptors, CD4 and CDS, which are involved in the interaction with their ligands3,4. As CD4 is expressed on the early haemopoietic progenitor5 as well as the early thymic precursor cells6, a role for CD4 in haemopoiesis and T-cell development is implicated. Thymocytes undergo a series of differentiation7 and selection steps8,9 to become mature CD4+8− or CD4−8+ (single positive) T cells10,11. Studies of the role of CD4+ T cells in vivo have been based on adoptive transfer of selected or depleted lymphocytes, or in vivo treatment of thymectomized mice with monoclonal antibodies causing depletion of CD4+ T cells12–14. In order to study the role of the CD4 molecule in the development and function of lymphocytes, we have disrupted the CD4 gene in embryonic stem cells15–16 by homologous recombination17–18. Germ-line transmission19–20 of the mutation produces mutant mouse strains that do not express CD4 on the cell surface. In these mice, the development of CD8+ T cells and myeloid components is unaltered, indicating that expression of CD4 on progenitor cells and CD4+ CD8+ (double positive) thymocytes is not obligatory. Here we report that these mice have markedly decreased helper cell activity for antibody responses, although cytotoxic T-cell activity against viruses is in the normal range. This differential requirement for CD4+ helper T cells is important to our understanding of immune disorders, including AIDS, in which CD4+ cells are reduced or absent.
40 schema:genre article
41 schema:isAccessibleForFree false
42 schema:isPartOf N8b2c4e8967e84fb8adb44bcbc6d8189a
43 Na09c538190b3477eb390b14d4f552705
44 sg:journal.1018957
45 schema:keywords AIDS
46 CD4
47 CD4 gene
48 CD4 molecule
49 CD8
50 CDS
51 T cell activity
52 T cell antigen receptor
53 T cell development
54 T cells
55 activity
56 adoptive transfer
57 antibodies
58 antibody response
59 antigen
60 antigen receptor
61 cell activity
62 cell surface
63 cells
64 cells6
65 components
66 conjunction
67 coreceptor
68 depletion
69 depletion of CD4
70 development
71 development of CD8
72 differential requirement
73 disorders
74 expression
75 expression of CD4
76 function
77 function of CD8
78 function of lymphocytes
79 genes
80 haemopoiesis
81 helper T cells
82 helper cell activity
83 histocompatibility
84 immune disorders
85 interaction
86 lymphocytes
87 major histocompatibility
88 mature CD4
89 mice
90 molecules
91 monoclonal antibodies
92 mouse strains
93 mutant mouse strains
94 mutations
95 myeloid component
96 normal development
97 normal range
98 order
99 products
100 progenitor cells
101 range
102 receptors
103 recognition
104 recognition of antigen
105 requirements
106 response
107 role
108 role of CD4
109 selection
110 series
111 strains
112 study
113 surface
114 thymocytes
115 transfer
116 treatment
117 understanding
118 virus
119 vivo
120 vivo treatment
121 schema:name Normal development and function of CD8+ cells but markedly decreased helper cell activity in mice lacking CD4
122 schema:pagination 180-184
123 schema:productId N59533fb626ab49fabb7703c21a030d0c
124 N7a7a93b7fc614d2da758d412055c073a
125 N9db7ef1d58a745f8a955126aaaa373ed
126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037128186
127 https://doi.org/10.1038/353180a0
128 schema:sdDatePublished 2022-11-24T20:47
129 schema:sdLicense https://scigraph.springernature.com/explorer/license/
130 schema:sdPublisher N47186e21422b4b7bb1fef1319a47d60a
131 schema:url https://doi.org/10.1038/353180a0
132 sgo:license sg:explorer/license/
133 sgo:sdDataset articles
134 rdf:type schema:ScholarlyArticle
135 N114c729ca2ed415eb004c0fd967ec0f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Lymph Nodes
137 rdf:type schema:DefinedTerm
138 N158950050b274e8b91071f5b527fc2b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name T-Lymphocytes, Helper-Inducer
140 rdf:type schema:DefinedTerm
141 N15fbeea669574e66b7bf10f0508b75e3 rdf:first sg:person.01137533300.95
142 rdf:rest N3839a2e69b5f4e6888faea9765378731
143 N22a493e916574eab8ba779ec4f396b04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Interleukin-2
145 rdf:type schema:DefinedTerm
146 N29fc4a1b267d47c2a72032e43cf68819 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Base Sequence
148 rdf:type schema:DefinedTerm
149 N3839a2e69b5f4e6888faea9765378731 rdf:first sg:person.01135675570.01
150 rdf:rest Nf4bbb366593e4705af0608a0de6996f8
151 N44bd8d0ef83b451d86628d2d2fbc2c48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Cell Differentiation
153 rdf:type schema:DefinedTerm
154 N47186e21422b4b7bb1fef1319a47d60a schema:name Springer Nature - SN SciGraph project
155 rdf:type schema:Organization
156 N48cdd1760fc54a929e169189b7f5bab6 rdf:first Nf1298290413341059c1b9dda9417e6f7
157 rdf:rest Na56bc4866f454353ba64d0bef30d2458
158 N493dd507787d40e386d47703e95d2e2d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Molecular Sequence Data
160 rdf:type schema:DefinedTerm
161 N59533fb626ab49fabb7703c21a030d0c schema:name dimensions_id
162 schema:value pub.1037128186
163 rdf:type schema:PropertyValue
164 N5a175fc0432d4f1393034d8fbd09754b rdf:first sg:person.0632551050.92
165 rdf:rest N5bf2a82f08ea418ba2570a145718706b
166 N5bf2a82f08ea418ba2570a145718706b rdf:first sg:person.01013477301.11
167 rdf:rest N65aebfecc86e4996ae04def46b30f5b2
168 N5c4c13df16f049d8ab69295ad7509bb3 rdf:first sg:person.01003673072.23
169 rdf:rest Nb8f0f5b53d9c4d0bbdba3f2e4cbda82d
170 N65aebfecc86e4996ae04def46b30f5b2 rdf:first sg:person.0751315141.50
171 rdf:rest N5c4c13df16f049d8ab69295ad7509bb3
172 N7a7a93b7fc614d2da758d412055c073a schema:name doi
173 schema:value 10.1038/353180a0
174 rdf:type schema:PropertyValue
175 N8b2c4e8967e84fb8adb44bcbc6d8189a schema:volumeNumber 353
176 rdf:type schema:PublicationVolume
177 N8d43e25731184913b87cb3efde7d4ed9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Antibody Formation
179 rdf:type schema:DefinedTerm
180 N9aab11589aab4bd9be5fcbf576e259f5 rdf:first sg:person.014440054532.58
181 rdf:rest rdf:nil
182 N9db7ef1d58a745f8a955126aaaa373ed schema:name pubmed_id
183 schema:value 1832488
184 rdf:type schema:PropertyValue
185 Na09c538190b3477eb390b14d4f552705 schema:issueNumber 6340
186 rdf:type schema:PublicationIssue
187 Na32bc5992ed0453ebcfdd4dac48ac9dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
188 schema:name DNA Mutational Analysis
189 rdf:type schema:DefinedTerm
190 Na56bc4866f454353ba64d0bef30d2458 rdf:first sg:person.0677274536.49
191 rdf:rest N15fbeea669574e66b7bf10f0508b75e3
192 Na59a8c8a9a074540a68c627d808940f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
193 schema:name Mice
194 rdf:type schema:DefinedTerm
195 Nb8f0f5b53d9c4d0bbdba3f2e4cbda82d rdf:first sg:person.0644157012.41
196 rdf:rest N48cdd1760fc54a929e169189b7f5bab6
197 Nb9d71d0f946f4e1eb09dcfa078d83c3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
198 schema:name T-Lymphocyte Subsets
199 rdf:type schema:DefinedTerm
200 Nbf8fe3f39331486e91d47a0357aa9ee8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
201 schema:name Oligonucleotides
202 rdf:type schema:DefinedTerm
203 Nc3b00b7e975042b5a260cd7235d0a64a rdf:first sg:person.0746777450.19
204 rdf:rest N5a175fc0432d4f1393034d8fbd09754b
205 Nc595e2f0f66445c6b2258aa1bb6eefe4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
206 schema:name Polymerase Chain Reaction
207 rdf:type schema:DefinedTerm
208 Nc6107f180cfa402bab3a18116c5f0cc2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
209 schema:name Spleen
210 rdf:type schema:DefinedTerm
211 Nc899ae02504e4e17be3e4cc08e83fee5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
212 schema:name Animals
213 rdf:type schema:DefinedTerm
214 Ne2152242a2dd433689ea63864c3a6a51 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
215 schema:name Cytotoxicity, Immunologic
216 rdf:type schema:DefinedTerm
217 Ne23d39bfb56a4eedb9ebed0d986ada6f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
218 schema:name Thymus Gland
219 rdf:type schema:DefinedTerm
220 Nea78fd3af6f549c499e39d1920ffbe3f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
221 schema:name Mice, Mutant Strains
222 rdf:type schema:DefinedTerm
223 Neac1f9da1e884c33af330eea12138d9c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
224 schema:name Lymphocyte Culture Test, Mixed
225 rdf:type schema:DefinedTerm
226 Nf1298290413341059c1b9dda9417e6f7 schema:affiliation grid-institutes:grid.17063.33
227 schema:familyName Arabian
228 schema:givenName A.
229 rdf:type schema:Person
230 Nf4bbb366593e4705af0608a0de6996f8 rdf:first sg:person.01120655377.49
231 rdf:rest N9aab11589aab4bd9be5fcbf576e259f5
232 Nf9b3cb0cf8d846ef96c9efa45a248595 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
233 schema:name CD4 Antigens
234 rdf:type schema:DefinedTerm
235 Nfae2ea70605940b38606b2ad09e357e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
236 schema:name Flow Cytometry
237 rdf:type schema:DefinedTerm
238 Nfcdd288e1d114017ae95d92c8ba3ca68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
239 schema:name T-Lymphocytes, Cytotoxic
240 rdf:type schema:DefinedTerm
241 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
242 schema:name Medical and Health Sciences
243 rdf:type schema:DefinedTerm
244 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
245 schema:name Immunology
246 rdf:type schema:DefinedTerm
247 sg:journal.1018957 schema:issn 0028-0836
248 1476-4687
249 schema:name Nature
250 schema:publisher Springer Nature
251 rdf:type schema:Periodical
252 sg:person.01003673072.23 schema:affiliation grid-institutes:grid.17063.33
253 schema:familyName Sambhara
254 schema:givenName S. R.
255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003673072.23
256 rdf:type schema:Person
257 sg:person.01013477301.11 schema:affiliation grid-institutes:grid.17063.33
258 schema:familyName Schilham
259 schema:givenName M. W.
260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01013477301.11
261 rdf:type schema:Person
262 sg:person.01120655377.49 schema:affiliation grid-institutes:grid.17063.33
263 schema:familyName Miller
264 schema:givenName R. G.
265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01120655377.49
266 rdf:type schema:Person
267 sg:person.01135675570.01 schema:affiliation grid-institutes:grid.17063.33
268 schema:familyName Zinkernagel
269 schema:givenName R. M.
270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01135675570.01
271 rdf:type schema:Person
272 sg:person.01137533300.95 schema:affiliation grid-institutes:grid.17063.33
273 schema:familyName Paige
274 schema:givenName C. J.
275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137533300.95
276 rdf:type schema:Person
277 sg:person.014440054532.58 schema:affiliation grid-institutes:grid.17063.33
278 schema:familyName Mak
279 schema:givenName T. W.
280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014440054532.58
281 rdf:type schema:Person
282 sg:person.0632551050.92 schema:affiliation grid-institutes:grid.17063.33
283 schema:familyName Fung-Leung
284 schema:givenName W. P.
285 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632551050.92
286 rdf:type schema:Person
287 sg:person.0644157012.41 schema:affiliation grid-institutes:grid.17063.33
288 schema:familyName Narendran
289 schema:givenName A.
290 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0644157012.41
291 rdf:type schema:Person
292 sg:person.0677274536.49 schema:affiliation grid-institutes:grid.17063.33
293 schema:familyName Wakeham
294 schema:givenName A.
295 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677274536.49
296 rdf:type schema:Person
297 sg:person.0746777450.19 schema:affiliation grid-institutes:grid.17063.33
298 schema:familyName Rahemtulla
299 schema:givenName A.
300 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746777450.19
301 rdf:type schema:Person
302 sg:person.0751315141.50 schema:affiliation grid-institutes:grid.17063.33
303 schema:familyName Kündig
304 schema:givenName T. M.
305 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751315141.50
306 rdf:type schema:Person
307 sg:pub.10.1038/276829a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015371060
308 https://doi.org/10.1038/276829a0
309 rdf:type schema:CreativeWork
310 sg:pub.10.1038/292154a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017487986
311 https://doi.org/10.1038/292154a0
312 rdf:type schema:CreativeWork
313 sg:pub.10.1038/308145a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021425337
314 https://doi.org/10.1038/308145a0
315 rdf:type schema:CreativeWork
316 sg:pub.10.1038/308149a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050508510
317 https://doi.org/10.1038/308149a0
318 rdf:type schema:CreativeWork
319 sg:pub.10.1038/312548a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003114730
320 https://doi.org/10.1038/312548a0
321 rdf:type schema:CreativeWork
322 sg:pub.10.1038/317230a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005647127
323 https://doi.org/10.1038/317230a0
324 rdf:type schema:CreativeWork
325 sg:pub.10.1038/323445a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047908913
326 https://doi.org/10.1038/323445a0
327 rdf:type schema:CreativeWork
328 sg:pub.10.1038/328077a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005872580
329 https://doi.org/10.1038/328077a0
330 rdf:type schema:CreativeWork
331 sg:pub.10.1038/333742a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018116885
332 https://doi.org/10.1038/333742a0
333 rdf:type schema:CreativeWork
334 sg:pub.10.1038/336684a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038677733
335 https://doi.org/10.1038/336684a0
336 rdf:type schema:CreativeWork
337 sg:pub.10.1038/338153a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004411576
338 https://doi.org/10.1038/338153a0
339 rdf:type schema:CreativeWork
340 sg:pub.10.1038/349071a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000779593
341 https://doi.org/10.1038/349071a0
342 rdf:type schema:CreativeWork
343 grid-institutes:grid.17063.33 schema:alternateName Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada
344 schema:name Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, 500 Sherbourne Street, M4X 1K9, Toronto, Ontario, Canada
345 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...