Accurate prediction of the stability and activity effects of site-directed mutagenesis on a protein core View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-08

AUTHORS

C Lee, M Levitt

ABSTRACT

Theoretical prediction of the structure, stability and activity of proteins, an important unsolved problem in molecular biology, would be of use for guiding site-directed mutagenesis and other protein-engineering techniques. X-ray diffraction studies have provided extensive structural information for many proteins, challenging theorists to develop reliable techniques able to use such knowledge as a base for prediction of mutants' characteristics. Here we report theoretical calculation of stabilization energies for 78 triple-site sequence variants of lambda repressor characterized experimentally by Lim and Sauer. The calculated energies correlate with the mutants' measured activities; active and inactive mutations are discriminated with 92% reliability. They correlate even more directly with the mutants' thermostabilities, correctly identifying two of the mutants to be more stable than the wild type. More... »

PAGES

448-451

Journal

TITLE

Nature

ISSUE

6334

VOLUME

352

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/352448a0

DOI

http://dx.doi.org/10.1038/352448a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013184073

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1861725


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