The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-09

AUTHORS

Chad A. Cowan, Mark Henkemeyer

ABSTRACT

Bidirectional signals mediated by membrane-anchored ephrins and Eph receptor tyrosine kinases have important functions in cell-cell recognition events, including those that occur during axon pathfinding and hindbrain segmentation. The reverse signal that is transduced into B-ephrin-expressing cells is thought to involve tyrosine phosphorylation of the signal's short, conserved carboxy-terminal cytoplasmic domain. The Src-homology-2 (SH2) domain proteins that associate with activated tyrosine-phosphorylated B-subclass ephrins have not been identified, nor has a defined cellular response to reverse signals been described. Here we show that the SH2/SH3 domain adaptor protein Grb4 binds to the cytoplasmic domain of B ephrins in a phosphotyrosine-dependent manner. In response to B-ephrin reverse signalling, cells increase FAK catalytic activity, redistribute paxillin, lose focal adhesions, round up, and disassemble F-actin-containing stress fibres. These cellular responses can be blocked in a dominant-negative fashion by expression of the isolated Grb4 SH2 domain. The Grb4 SH3 domains bind a unique set of other proteins that are implicated in cytoskeletal regulation, including the Cbl-associated protein (CAP/ponsin), the Abl-interacting protein-1 (Abi-1), dynamin, PAK1, hnRNPK and axin. These data provide a biochemical pathway whereby cytoskeletal regulators are recruited to Eph-ephrin bidirectional signalling complexes. More... »

PAGES

174

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/35093123

DOI

http://dx.doi.org/10.1038/35093123

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016114423

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11557983


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adaptor Proteins, Signal Transducing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Axin Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoskeletal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoskeleton", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ephrin-B1", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glutathione Transferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microfilament Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphotyrosine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptor Protein-Tyrosine Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptor, EphB4", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptors, Eph Family", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Fusion Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Repressor Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Signal Transduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Two-Hybrid System Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "src Homology Domains", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Center for Developmental Biology and Kent Waldrep Foundation Center for Basic Research on Nerve Growth and Regeneration, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cowan", 
        "givenName": "Chad A.", 
        "id": "sg:person.010233243157.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010233243157.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas Southwestern Medical Center", 
          "id": "https://www.grid.ac/institutes/grid.267313.2", 
          "name": [
            "Center for Developmental Biology and Kent Waldrep Foundation Center for Basic Research on Nerve Growth and Regeneration, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Henkemeyer", 
        "givenName": "Mark", 
        "id": "sg:person.01322520744.87", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322520744.87"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/mcb.20.21.7867-7880.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001474203"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/383722a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001993422", 
          "https://doi.org/10.1038/383722a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/dbio.1995.1072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006458919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80324-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006523900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1998.tb00657.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007829625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1998.tb00657.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007829625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(06)00052-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008962971"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81231-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009387169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/20452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009706917", 
          "https://doi.org/10.1038/20452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/20452", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009706917", 
          "https://doi.org/10.1038/20452"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.39.25171", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014007320"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)80075-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023361909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0898-6568(98)00027-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024431202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/21907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024487580", 
          "https://doi.org/10.1038/21907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/21907", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024487580", 
          "https://doi.org/10.1038/21907"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6107(98)00052-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026771490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.13.23.3125", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030144536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2443.1999.00283.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032462720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.89.19.8894", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034706954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.9.21.2583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038270489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(98)70249-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038711233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.144.5.1001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040104385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.7.4073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040330491"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/emboj/17.5.1371", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041267201"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(00)81131-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042426600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.15.3.1324", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044911784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.9.21.2569", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052653640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.9.12.3367", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053623994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.275.5306.1640", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062556059"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.283.5408.1739", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062564489"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.8438166", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062655600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074593202", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082627485", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082883115", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2001-09", 
    "datePublishedReg": "2001-09-01", 
    "description": "Bidirectional signals mediated by membrane-anchored ephrins and Eph receptor tyrosine kinases have important functions in cell-cell recognition events, including those that occur during axon pathfinding and hindbrain segmentation. The reverse signal that is transduced into B-ephrin-expressing cells is thought to involve tyrosine phosphorylation of the signal's short, conserved carboxy-terminal cytoplasmic domain. The Src-homology-2 (SH2) domain proteins that associate with activated tyrosine-phosphorylated B-subclass ephrins have not been identified, nor has a defined cellular response to reverse signals been described. Here we show that the SH2/SH3 domain adaptor protein Grb4 binds to the cytoplasmic domain of B ephrins in a phosphotyrosine-dependent manner. In response to B-ephrin reverse signalling, cells increase FAK catalytic activity, redistribute paxillin, lose focal adhesions, round up, and disassemble F-actin-containing stress fibres. These cellular responses can be blocked in a dominant-negative fashion by expression of the isolated Grb4 SH2 domain. The Grb4 SH3 domains bind a unique set of other proteins that are implicated in cytoskeletal regulation, including the Cbl-associated protein (CAP/ponsin), the Abl-interacting protein-1 (Abi-1), dynamin, PAK1, hnRNPK and axin. These data provide a biochemical pathway whereby cytoskeletal regulators are recruited to Eph-ephrin bidirectional signalling complexes.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/35093123", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6852", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "413"
      }
    ], 
    "name": "The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals", 
    "pagination": "174", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4cc7e0e34264034c4097697bb8d9def005cf938db5bd91e7ee810d33d2b2e15b"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11557983"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/35093123"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1016114423"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/35093123", 
      "https://app.dimensions.ai/details/publication/pub.1016114423"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87117_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/35093123"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/35093123'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/35093123'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/35093123'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/35093123'


 

This table displays all metadata directly associated to this object as RDF triples.

281 TRIPLES      21 PREDICATES      88 URIs      49 LITERALS      37 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/35093123 schema:about N05c05e85e25d40938468c08f256740f8
2 N1edda39b7515434888faed95529ea2c1
3 N20ea407dd39b41b99a023647bec17048
4 N2b64f71e8a854bb589bf4a6d2b8a48aa
5 N35c5f8bd936c40cd8c0954de29e700d4
6 N386b659be217448d8f270fd4a344901a
7 N3c2bae1d44634346b6a7cd9746ae444c
8 N3d031eabc3764874acb497ad0693f077
9 N3d3a7c5d67a845719f3f4abc9b4eb95b
10 N3eb2820bc4bd40c49e567a0922b9fbdd
11 N400d55d0fa9b40c6abc9c7c0fd219489
12 N4a3098c67ac54ba6a486b5b3426bf483
13 N4d77322b8a14442283e87b7e2b10178b
14 N5ae7001dc6694bf98395807e11f04b72
15 N62fd466f47c641c3b87264b459e292df
16 N79c31810aac74684afd9574b64969fca
17 N823b6803786c432ab7dfe42069d5cbbe
18 N890b4d32e6d84547bf1cf1548bef1bac
19 Nb2c4216d04b545729bae67d2f9e39491
20 Nb4430fff40e44c7c818ef080292da518
21 Nbdb9427de7d6491bb41aa661281b20e6
22 Nd1d5f477a7ac44c8a3b30491b8b1affd
23 Nd53e44cc6b73438db37d75338666509a
24 Nd67daac26bcc4522b2f3c9e58cbe0333
25 Ndcdf3afc096a4c0fac7dba9d0966b8bb
26 Ne2ac523a65414b61b4e365f38df195f6
27 Ne60f696c19aa432b9be57ce5c34a733d
28 Nff89566c62c8497696ee7dc8b436e28d
29 anzsrc-for:06
30 anzsrc-for:0601
31 schema:author N0932cfadb212469283092188cea74525
32 schema:citation sg:pub.10.1038/20452
33 sg:pub.10.1038/21907
34 sg:pub.10.1038/383722a0
35 https://app.dimensions.ai/details/publication/pub.1074593202
36 https://app.dimensions.ai/details/publication/pub.1082627485
37 https://app.dimensions.ai/details/publication/pub.1082883115
38 https://doi.org/10.1006/dbio.1995.1072
39 https://doi.org/10.1016/s0079-6107(98)00052-2
40 https://doi.org/10.1016/s0092-8674(00)80075-6
41 https://doi.org/10.1016/s0092-8674(00)80324-4
42 https://doi.org/10.1016/s0092-8674(00)81131-9
43 https://doi.org/10.1016/s0092-8674(00)81231-3
44 https://doi.org/10.1016/s0898-6568(98)00027-8
45 https://doi.org/10.1016/s0960-9822(06)00052-2
46 https://doi.org/10.1016/s0960-9822(98)70249-0
47 https://doi.org/10.1046/j.1365-2443.1999.00283.x
48 https://doi.org/10.1073/pnas.89.19.8894
49 https://doi.org/10.1074/jbc.273.39.25171
50 https://doi.org/10.1074/jbc.273.7.4073
51 https://doi.org/10.1083/jcb.144.5.1001
52 https://doi.org/10.1091/mbc.9.12.3367
53 https://doi.org/10.1093/emboj/17.5.1371
54 https://doi.org/10.1101/gad.13.23.3125
55 https://doi.org/10.1101/gad.9.21.2569
56 https://doi.org/10.1101/gad.9.21.2583
57 https://doi.org/10.1111/j.1399-3011.1998.tb00657.x
58 https://doi.org/10.1126/science.275.5306.1640
59 https://doi.org/10.1126/science.283.5408.1739
60 https://doi.org/10.1126/science.8438166
61 https://doi.org/10.1128/mcb.15.3.1324
62 https://doi.org/10.1128/mcb.20.21.7867-7880.2000
63 schema:datePublished 2001-09
64 schema:datePublishedReg 2001-09-01
65 schema:description Bidirectional signals mediated by membrane-anchored ephrins and Eph receptor tyrosine kinases have important functions in cell-cell recognition events, including those that occur during axon pathfinding and hindbrain segmentation. The reverse signal that is transduced into B-ephrin-expressing cells is thought to involve tyrosine phosphorylation of the signal's short, conserved carboxy-terminal cytoplasmic domain. The Src-homology-2 (SH2) domain proteins that associate with activated tyrosine-phosphorylated B-subclass ephrins have not been identified, nor has a defined cellular response to reverse signals been described. Here we show that the SH2/SH3 domain adaptor protein Grb4 binds to the cytoplasmic domain of B ephrins in a phosphotyrosine-dependent manner. In response to B-ephrin reverse signalling, cells increase FAK catalytic activity, redistribute paxillin, lose focal adhesions, round up, and disassemble F-actin-containing stress fibres. These cellular responses can be blocked in a dominant-negative fashion by expression of the isolated Grb4 SH2 domain. The Grb4 SH3 domains bind a unique set of other proteins that are implicated in cytoskeletal regulation, including the Cbl-associated protein (CAP/ponsin), the Abl-interacting protein-1 (Abi-1), dynamin, PAK1, hnRNPK and axin. These data provide a biochemical pathway whereby cytoskeletal regulators are recruited to Eph-ephrin bidirectional signalling complexes.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree false
69 schema:isPartOf N55ba42442c00490cab06273f977b3851
70 N62bee2f8c0854191bb0b1f5f4487a38e
71 sg:journal.1018957
72 schema:name The SH2/SH3 adaptor Grb4 transduces B-ephrin reverse signals
73 schema:pagination 174
74 schema:productId N30970373db6c4531b521f0aa052c25a8
75 N42ea95db87d14f3189c66604e735ccf5
76 N8462e274dd3e4046a722763bdf716c9b
77 Na5f7c171db184cae88d5066ad65b53bf
78 Ndd8faa07aeff42729ee864e7b47a4ebd
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016114423
80 https://doi.org/10.1038/35093123
81 schema:sdDatePublished 2019-04-11T12:27
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher N5e248494979a45a2846c5297f8d5863c
84 schema:url https://www.nature.com/articles/35093123
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N05c05e85e25d40938468c08f256740f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Ephrin-B1
90 rdf:type schema:DefinedTerm
91 N0932cfadb212469283092188cea74525 rdf:first sg:person.010233243157.25
92 rdf:rest Nd4fea732e1f249929c73f7881a6a7ddc
93 N1edda39b7515434888faed95529ea2c1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Cytoskeletal Proteins
95 rdf:type schema:DefinedTerm
96 N20ea407dd39b41b99a023647bec17048 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Microfilament Proteins
98 rdf:type schema:DefinedTerm
99 N2b64f71e8a854bb589bf4a6d2b8a48aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Repressor Proteins
101 rdf:type schema:DefinedTerm
102 N30970373db6c4531b521f0aa052c25a8 schema:name dimensions_id
103 schema:value pub.1016114423
104 rdf:type schema:PropertyValue
105 N35c5f8bd936c40cd8c0954de29e700d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Adaptor Proteins, Signal Transducing
107 rdf:type schema:DefinedTerm
108 N386b659be217448d8f270fd4a344901a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Oncogene Proteins
110 rdf:type schema:DefinedTerm
111 N3c2bae1d44634346b6a7cd9746ae444c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Membrane Proteins
113 rdf:type schema:DefinedTerm
114 N3d031eabc3764874acb497ad0693f077 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Receptor Protein-Tyrosine Kinases
116 rdf:type schema:DefinedTerm
117 N3d3a7c5d67a845719f3f4abc9b4eb95b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name src Homology Domains
119 rdf:type schema:DefinedTerm
120 N3eb2820bc4bd40c49e567a0922b9fbdd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Phosphotyrosine
122 rdf:type schema:DefinedTerm
123 N400d55d0fa9b40c6abc9c7c0fd219489 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Proteins
125 rdf:type schema:DefinedTerm
126 N42ea95db87d14f3189c66604e735ccf5 schema:name nlm_unique_id
127 schema:value 0410462
128 rdf:type schema:PropertyValue
129 N4a3098c67ac54ba6a486b5b3426bf483 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Receptor, EphB4
131 rdf:type schema:DefinedTerm
132 N4d77322b8a14442283e87b7e2b10178b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Glutathione Transferase
134 rdf:type schema:DefinedTerm
135 N55ba42442c00490cab06273f977b3851 schema:volumeNumber 413
136 rdf:type schema:PublicationVolume
137 N5ae7001dc6694bf98395807e11f04b72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Recombinant Fusion Proteins
139 rdf:type schema:DefinedTerm
140 N5e248494979a45a2846c5297f8d5863c schema:name Springer Nature - SN SciGraph project
141 rdf:type schema:Organization
142 N62bee2f8c0854191bb0b1f5f4487a38e schema:issueNumber 6852
143 rdf:type schema:PublicationIssue
144 N62fd466f47c641c3b87264b459e292df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Cell Line
146 rdf:type schema:DefinedTerm
147 N79c31810aac74684afd9574b64969fca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Mice
149 rdf:type schema:DefinedTerm
150 N823b6803786c432ab7dfe42069d5cbbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Receptors, Eph Family
152 rdf:type schema:DefinedTerm
153 N8462e274dd3e4046a722763bdf716c9b schema:name readcube_id
154 schema:value 4cc7e0e34264034c4097697bb8d9def005cf938db5bd91e7ee810d33d2b2e15b
155 rdf:type schema:PropertyValue
156 N890b4d32e6d84547bf1cf1548bef1bac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Protein Binding
158 rdf:type schema:DefinedTerm
159 Na5f7c171db184cae88d5066ad65b53bf schema:name pubmed_id
160 schema:value 11557983
161 rdf:type schema:PropertyValue
162 Nb2c4216d04b545729bae67d2f9e39491 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Humans
164 rdf:type schema:DefinedTerm
165 Nb4430fff40e44c7c818ef080292da518 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Amino Acid Sequence
167 rdf:type schema:DefinedTerm
168 Nbdb9427de7d6491bb41aa661281b20e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Cytoskeleton
170 rdf:type schema:DefinedTerm
171 Nd1d5f477a7ac44c8a3b30491b8b1affd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Cell Membrane
173 rdf:type schema:DefinedTerm
174 Nd4fea732e1f249929c73f7881a6a7ddc rdf:first sg:person.01322520744.87
175 rdf:rest rdf:nil
176 Nd53e44cc6b73438db37d75338666509a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Signal Transduction
178 rdf:type schema:DefinedTerm
179 Nd67daac26bcc4522b2f3c9e58cbe0333 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Animals
181 rdf:type schema:DefinedTerm
182 Ndcdf3afc096a4c0fac7dba9d0966b8bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Two-Hybrid System Techniques
184 rdf:type schema:DefinedTerm
185 Ndd8faa07aeff42729ee864e7b47a4ebd schema:name doi
186 schema:value 10.1038/35093123
187 rdf:type schema:PropertyValue
188 Ne2ac523a65414b61b4e365f38df195f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Axin Protein
190 rdf:type schema:DefinedTerm
191 Ne60f696c19aa432b9be57ce5c34a733d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Molecular Sequence Data
193 rdf:type schema:DefinedTerm
194 Nff89566c62c8497696ee7dc8b436e28d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
195 schema:name Neurons
196 rdf:type schema:DefinedTerm
197 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
198 schema:name Biological Sciences
199 rdf:type schema:DefinedTerm
200 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
201 schema:name Biochemistry and Cell Biology
202 rdf:type schema:DefinedTerm
203 sg:journal.1018957 schema:issn 0090-0028
204 1476-4687
205 schema:name Nature
206 rdf:type schema:Periodical
207 sg:person.010233243157.25 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
208 schema:familyName Cowan
209 schema:givenName Chad A.
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010233243157.25
211 rdf:type schema:Person
212 sg:person.01322520744.87 schema:affiliation https://www.grid.ac/institutes/grid.267313.2
213 schema:familyName Henkemeyer
214 schema:givenName Mark
215 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322520744.87
216 rdf:type schema:Person
217 sg:pub.10.1038/20452 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009706917
218 https://doi.org/10.1038/20452
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/21907 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024487580
221 https://doi.org/10.1038/21907
222 rdf:type schema:CreativeWork
223 sg:pub.10.1038/383722a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001993422
224 https://doi.org/10.1038/383722a0
225 rdf:type schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1074593202 schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1082627485 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1082883115 schema:CreativeWork
229 https://doi.org/10.1006/dbio.1995.1072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006458919
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0079-6107(98)00052-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026771490
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/s0092-8674(00)80075-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023361909
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/s0092-8674(00)80324-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006523900
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0092-8674(00)81131-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042426600
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0092-8674(00)81231-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009387169
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0898-6568(98)00027-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024431202
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0960-9822(06)00052-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008962971
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1016/s0960-9822(98)70249-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038711233
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1046/j.1365-2443.1999.00283.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1032462720
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1073/pnas.89.19.8894 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034706954
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1074/jbc.273.39.25171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014007320
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1074/jbc.273.7.4073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040330491
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1083/jcb.144.5.1001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040104385
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1091/mbc.9.12.3367 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053623994
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1093/emboj/17.5.1371 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041267201
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1101/gad.13.23.3125 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030144536
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1101/gad.9.21.2569 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052653640
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1101/gad.9.21.2583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038270489
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1111/j.1399-3011.1998.tb00657.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007829625
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1126/science.275.5306.1640 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062556059
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1126/science.283.5408.1739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062564489
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1126/science.8438166 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062655600
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1128/mcb.15.3.1324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044911784
276 rdf:type schema:CreativeWork
277 https://doi.org/10.1128/mcb.20.21.7867-7880.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001474203
278 rdf:type schema:CreativeWork
279 https://www.grid.ac/institutes/grid.267313.2 schema:alternateName The University of Texas Southwestern Medical Center
280 schema:name Center for Developmental Biology and Kent Waldrep Foundation Center for Basic Research on Nerve Growth and Regeneration, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9133, USA
281 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...