Functional proteins from a random-sequence library View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2001-04

AUTHORS

Anthony D. Keefe, Jack W. Szostak

ABSTRACT

Functional primordial proteins presumably originated from random sequences, but it is not known how frequently functional, or even folded, proteins occur in collections of random sequences. Here we have used in vitro selection of messenger RNA displayed proteins, in which each protein is covalently linked through its carboxy terminus to the 3' end of its encoding mRNA, to sample a large number of distinct random sequences. Starting from a library of 6 x 1012 proteins each containing 80 contiguous random amino acids, we selected functional proteins by enriching for those that bind to ATP. This selection yielded four new ATP-binding proteins that appear to be unrelated to each other or to anything found in the current databases of biological proteins. The frequency of occurrence of functional proteins in random-sequence libraries appears to be similar to that observed for equivalent RNA libraries. More... »

PAGES

715

Journal

TITLE

Nature

ISSUE

6829

VOLUME

410

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/35070613

DOI

http://dx.doi.org/10.1038/35070613

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011729333

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11287961


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