A mediator required for activation of RNA polymerase II transcription in vitro View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-04

AUTHORS

P M Flanagan, R J Kelleher, M H Sayre, H Tschochner, R D Kornberg

ABSTRACT

Activator proteins bind to enhancer DNA elements and stimulate the initiation of transcription. It has been proposed that activators contact general initiation factors at a promoter, and evidence for such direct interaction has been obtained. Studies of transcription in vitro, however, have suggested that activators might function through an intermediary molecule(s) distinct from the general factors. In the first of these studies, we exploited the finding that one activator could inhibit transcription stimulated by a second activator (activator interference or 'squelching'). This inhibition, which is attributed to competition between the activators for a common target factor, could not be relieved by addition of a large excess of general initiation factors, suggesting that the target for which activators compete is distinct from these factors. Similar conclusions came from the observation that TFIID's expressed from cloned genes fail to replace partially purified 'natural' TFIID fractions in supporting activation, evidently because they lacked some component present in the impure fractions. While these lines of evidence for a novel 'mediator' of activation were negative, we also showed that a partially purified fraction from yeast would reverse activator interference. This positive effect of a presumptive mediator provided an assay for its activity, but its role in activation was still only inferred. We now present direct evidence for a mediator which is required for stimulation of transcription in vitro by the activators GAL4-VP16 and GCN4, but which has no effect on transcription in the absence of activator protein. More... »

PAGES

436-438

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/350436a0

DOI

http://dx.doi.org/10.1038/350436a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012013707

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2011193


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Polymerase II", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factor TFIID", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Stanford University", 
          "id": "https://www.grid.ac/institutes/grid.168010.e", 
          "name": [
            "Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, California 94305."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Flanagan", 
        "givenName": "P M", 
        "id": "sg:person.011201616104.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011201616104.07"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kelleher", 
        "givenName": "R J", 
        "id": "sg:person.01101125460.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101125460.02"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Sayre", 
        "givenName": "M H", 
        "id": "sg:person.01147240660.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147240660.05"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Tschochner", 
        "givenName": "H", 
        "id": "sg:person.01215354060.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215354060.35"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Kornberg", 
        "givenName": "R D", 
        "id": "sg:person.01367663056.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367663056.17"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1991-04", 
    "datePublishedReg": "1991-04-01", 
    "description": "Activator proteins bind to enhancer DNA elements and stimulate the initiation of transcription. It has been proposed that activators contact general initiation factors at a promoter, and evidence for such direct interaction has been obtained. Studies of transcription in vitro, however, have suggested that activators might function through an intermediary molecule(s) distinct from the general factors. In the first of these studies, we exploited the finding that one activator could inhibit transcription stimulated by a second activator (activator interference or 'squelching'). This inhibition, which is attributed to competition between the activators for a common target factor, could not be relieved by addition of a large excess of general initiation factors, suggesting that the target for which activators compete is distinct from these factors. Similar conclusions came from the observation that TFIID's expressed from cloned genes fail to replace partially purified 'natural' TFIID fractions in supporting activation, evidently because they lacked some component present in the impure fractions. While these lines of evidence for a novel 'mediator' of activation were negative, we also showed that a partially purified fraction from yeast would reverse activator interference. This positive effect of a presumptive mediator provided an assay for its activity, but its role in activation was still only inferred. We now present direct evidence for a mediator which is required for stimulation of transcription in vitro by the activators GAL4-VP16 and GCN4, but which has no effect on transcription in the absence of activator protein.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/350436a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6317", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "350"
      }
    ], 
    "name": "A mediator required for activation of RNA polymerase II transcription in vitro", 
    "pagination": "436-438", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "393d5342a3e747899773ef8b4c5d7e8d785e13ea059f86639d88e30343d8380d"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "2011193"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/350436a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1012013707"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/350436a0", 
      "https://app.dimensions.ai/details/publication/pub.1012013707"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T12:57", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000422.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nature/journal/v350/n6317/full/350436a0.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/350436a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/350436a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/350436a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/350436a0'


 

This table displays all metadata directly associated to this object as RDF triples.

141 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/350436a0 schema:about N0022dfe812c442fba273ac319345b14a
2 N15d81dc2583048fa96f3eb5e4dbc2a76
3 N1ebdecedea8144e29bd31a0e52a02b99
4 N75e46089457642b7850b646259ad5f9f
5 N8dfa3c79516840e78599eac73fa85fe8
6 N96c7854144124d5bba9d9aeac887849c
7 N9c0f31c864904e0e95c6859611623812
8 Na3c06b03ac19471ba58fcf6115f2f4e8
9 Nb0e6638ac8a64ce3a2fb62b9e816c6df
10 Nd3d1cdcb246d4c42846d2f1e803c1ddf
11 Ne9cef8229e914a3bb51b9b418740065d
12 Nf0f8412ce62d4250920ccf0ec05859d7
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N407e3cce47bb4721b53aac4b62726cb1
16 schema:datePublished 1991-04
17 schema:datePublishedReg 1991-04-01
18 schema:description Activator proteins bind to enhancer DNA elements and stimulate the initiation of transcription. It has been proposed that activators contact general initiation factors at a promoter, and evidence for such direct interaction has been obtained. Studies of transcription in vitro, however, have suggested that activators might function through an intermediary molecule(s) distinct from the general factors. In the first of these studies, we exploited the finding that one activator could inhibit transcription stimulated by a second activator (activator interference or 'squelching'). This inhibition, which is attributed to competition between the activators for a common target factor, could not be relieved by addition of a large excess of general initiation factors, suggesting that the target for which activators compete is distinct from these factors. Similar conclusions came from the observation that TFIID's expressed from cloned genes fail to replace partially purified 'natural' TFIID fractions in supporting activation, evidently because they lacked some component present in the impure fractions. While these lines of evidence for a novel 'mediator' of activation were negative, we also showed that a partially purified fraction from yeast would reverse activator interference. This positive effect of a presumptive mediator provided an assay for its activity, but its role in activation was still only inferred. We now present direct evidence for a mediator which is required for stimulation of transcription in vitro by the activators GAL4-VP16 and GCN4, but which has no effect on transcription in the absence of activator protein.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N39ded2d4795045adaa3c54faff84dfce
23 N9ff8a02d6c8544d7982a70cb637fb777
24 sg:journal.1018957
25 schema:name A mediator required for activation of RNA polymerase II transcription in vitro
26 schema:pagination 436-438
27 schema:productId N1c305348a010409e92789f856ecccae5
28 N326d424e97e34233871b9aeb18ba16ee
29 N788e5239365c4a129be43fe6ff1b4731
30 N7edbae75b24649e79c58ec8bc97430e5
31 N84fcf713af1f429baa75a5c5a2f8507d
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012013707
33 https://doi.org/10.1038/350436a0
34 schema:sdDatePublished 2019-04-10T12:57
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher Na119b07f16c840c8864cbbd926c2726d
37 schema:url http://www.nature.com/nature/journal/v350/n6317/full/350436a0.html
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N0022dfe812c442fba273ac319345b14a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name Transcription Factor TFIID
43 rdf:type schema:DefinedTerm
44 N15d81dc2583048fa96f3eb5e4dbc2a76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
45 schema:name Saccharomyces cerevisiae
46 rdf:type schema:DefinedTerm
47 N1c305348a010409e92789f856ecccae5 schema:name readcube_id
48 schema:value 393d5342a3e747899773ef8b4c5d7e8d785e13ea059f86639d88e30343d8380d
49 rdf:type schema:PropertyValue
50 N1ebdecedea8144e29bd31a0e52a02b99 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Saccharomyces cerevisiae Proteins
52 rdf:type schema:DefinedTerm
53 N31a1313a581d4205b8582d0e5b4cfa1f rdf:first sg:person.01367663056.17
54 rdf:rest rdf:nil
55 N326d424e97e34233871b9aeb18ba16ee schema:name dimensions_id
56 schema:value pub.1012013707
57 rdf:type schema:PropertyValue
58 N39ded2d4795045adaa3c54faff84dfce schema:issueNumber 6317
59 rdf:type schema:PublicationIssue
60 N407e3cce47bb4721b53aac4b62726cb1 rdf:first sg:person.011201616104.07
61 rdf:rest Na334a0f96c084b3eb67862609439f77f
62 N67aa90f30ebe436b80ccf36190c304ed rdf:first sg:person.01215354060.35
63 rdf:rest N31a1313a581d4205b8582d0e5b4cfa1f
64 N75e46089457642b7850b646259ad5f9f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name RNA Polymerase II
66 rdf:type schema:DefinedTerm
67 N788e5239365c4a129be43fe6ff1b4731 schema:name doi
68 schema:value 10.1038/350436a0
69 rdf:type schema:PropertyValue
70 N7edbae75b24649e79c58ec8bc97430e5 schema:name nlm_unique_id
71 schema:value 0410462
72 rdf:type schema:PropertyValue
73 N84fcf713af1f429baa75a5c5a2f8507d schema:name pubmed_id
74 schema:value 2011193
75 rdf:type schema:PropertyValue
76 N8dfa3c79516840e78599eac73fa85fe8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name DNA-Binding Proteins
78 rdf:type schema:DefinedTerm
79 N96c7854144124d5bba9d9aeac887849c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Recombinant Proteins
81 rdf:type schema:DefinedTerm
82 N9c0f31c864904e0e95c6859611623812 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name In Vitro Techniques
84 rdf:type schema:DefinedTerm
85 N9ff8a02d6c8544d7982a70cb637fb777 schema:volumeNumber 350
86 rdf:type schema:PublicationVolume
87 Na119b07f16c840c8864cbbd926c2726d schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 Na334a0f96c084b3eb67862609439f77f rdf:first sg:person.01101125460.02
90 rdf:rest Nda39a72f9cfc48e4b808c1b9f184457a
91 Na3c06b03ac19471ba58fcf6115f2f4e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Transcription Factors
93 rdf:type schema:DefinedTerm
94 Nb0e6638ac8a64ce3a2fb62b9e816c6df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Fungal Proteins
96 rdf:type schema:DefinedTerm
97 Nd3d1cdcb246d4c42846d2f1e803c1ddf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Nuclear Proteins
99 rdf:type schema:DefinedTerm
100 Nda39a72f9cfc48e4b808c1b9f184457a rdf:first sg:person.01147240660.05
101 rdf:rest N67aa90f30ebe436b80ccf36190c304ed
102 Ne9cef8229e914a3bb51b9b418740065d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Transcription, Genetic
104 rdf:type schema:DefinedTerm
105 Nf0f8412ce62d4250920ccf0ec05859d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Enzyme Activation
107 rdf:type schema:DefinedTerm
108 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
109 schema:name Biological Sciences
110 rdf:type schema:DefinedTerm
111 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
112 schema:name Biochemistry and Cell Biology
113 rdf:type schema:DefinedTerm
114 sg:journal.1018957 schema:issn 0090-0028
115 1476-4687
116 schema:name Nature
117 rdf:type schema:Periodical
118 sg:person.01101125460.02 schema:familyName Kelleher
119 schema:givenName R J
120 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01101125460.02
121 rdf:type schema:Person
122 sg:person.011201616104.07 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
123 schema:familyName Flanagan
124 schema:givenName P M
125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011201616104.07
126 rdf:type schema:Person
127 sg:person.01147240660.05 schema:familyName Sayre
128 schema:givenName M H
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147240660.05
130 rdf:type schema:Person
131 sg:person.01215354060.35 schema:familyName Tschochner
132 schema:givenName H
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215354060.35
134 rdf:type schema:Person
135 sg:person.01367663056.17 schema:familyName Kornberg
136 schema:givenName R D
137 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367663056.17
138 rdf:type schema:Person
139 https://www.grid.ac/institutes/grid.168010.e schema:alternateName Stanford University
140 schema:name Department of Cell Biology, Fairchild Center, Stanford University School of Medicine, California 94305.
141 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...