PKC-θ is required for TCR-induced NF-κB activation in mature but not immature T lymphocytes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-03

AUTHORS

Zuoming Sun, Christopher W. Arendt, Wilfried Ellmeier, Edward M. Schaeffer, Mary Jean Sunshine, Leena Gandhi, Justin Annes, Daniela Petrzilka, Abraham Kupfer, Pamela L. Schwartzberg, Dan R. Littman

ABSTRACT

Productive interaction of a T lymphocyte with an antigen-presenting cell results in the clustering of the T-cell antigen receptor (TCR) and the recruitment of a large signalling complex to the site of cell-cell contact. Subsequent signal transduction resulting in cytokine gene expression requires the activation of one or more of the multiple isoenzymes of serine/threonine-specific protein kinase C (PKC). Among the several PKC isoenzymes expressed in T cells, PKC-theta is unique in being rapidly recruited to the site of TCR clustering. Here we show that PKC-theta is essential for TCR-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. TCR-initiated NF-kappaB activation was absent from PKC-theta(-/-) mature T lymphocytes, but was intact in thymocytes. Activation of NF-kappaB by tumour-necrosis factor alpha and interleukin-1 was unaffected in the mutant mice. Although studies in T-cell lines had suggested that PKC-theta regulates activation of the JNK signalling pathway, induction of JNK was normal in T cells from mutant mice. These results indicate that PKC-theta functions in a unique pathway that links the TCR signalling complex to the activation of NF-kappaB in mature T lymphocytes. More... »

PAGES

402

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/35006090

DOI

http://dx.doi.org/10.1038/35006090

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023485125

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10746729


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