Specific interference with gene function by double-stranded RNA in early mouse development View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-12-23

AUTHORS

Florence Wianny, Magdalena Zernicka-Goetz

ABSTRACT

The use of double-stranded (ds) RNA is a powerful way of interfering with gene expression in a range of organisms, but doubts have been raised about whether it could be successful in mammals. Here, we show that dsRNA is effective as a specific inhibitor of the function of three genes in the mouse, namely maternally expressed c-mos in the oocyte and zygotically expressed E-cadherin or a GFP transgene in the preimplantation embryo. The phenotypes observed are the same as those reported for null mutants of the endogenous genes. These findings offer the opportunity to study development and gene regulation in normal and diseased cells. More... »

PAGES

70-75

Journal

TITLE

Nature Cell Biology

ISSUE

2

VOLUME

2

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  • Identifiers

    URI

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    DOI

    http://dx.doi.org/10.1038/35000016

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012242634

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10655585


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    29 schema:description The use of double-stranded (ds) RNA is a powerful way of interfering with gene expression in a range of organisms, but doubts have been raised about whether it could be successful in mammals. Here, we show that dsRNA is effective as a specific inhibitor of the function of three genes in the mouse, namely maternally expressed c-mos in the oocyte and zygotically expressed E-cadherin or a GFP transgene in the preimplantation embryo. The phenotypes observed are the same as those reported for null mutants of the endogenous genes. These findings offer the opportunity to study development and gene regulation in normal and diseased cells.
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