'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-01

AUTHORS

M. Gerard Waters, Tito Serafini, James E. Rothman

ABSTRACT

GOLGI-derived coated vesicles contain a set of coat proteins of relative molecular mass 160,000 (Mr 160K; α-COP), 110K (β-COP), 98K (γ-COP) and 61K (δ-COP), and several smaller subunits1. We have now identified and purified a cytosolic complex containing the same four coat proteins as those of Golgi transport vesicles. We term this complex the Golgi coat promoter or 'coatomer'. The coatomer also contains polypeptides of Mr 36K, 35K and 20K. It represents about 0.2% of soluble cytosolic protein. Gel filtration of unfractionated cytosol indicates that β-COP resides exclusively in the coatomer complex. The complex seems to be a likely candidate for the unassembled precursor of Golgi coated vesicles, and its purification should help investigations of the role of coat proteins in membrane budding, for which it is necessary to use a refined cell-free system. More... »

PAGES

248-251

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/349248a0

DOI

http://dx.doi.org/10.1038/349248a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048861040

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1898986


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