Base sequence discrimination by zinc-finger DNA-binding domains View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-01

AUTHORS

Jeannette Nardelli, Toby J. Gibson, Christine Vesque, Patrick Charnay

ABSTRACT

ZINC fingers1,2 constitute important eukaryotic DNA-binding domains, being present in many transcription factors3–5. The Cys2/His2zinc-finger class has conserved motifs of 28–30 amino acids which are usually present as tandem repeats1,2. The structure of a Cys2/His2zinc finger has been determined by nuclear magnetic resonance6, but details of its interaction with DNA were not established. Here we identify amino acids governing DNA-binding specificity using in vitro directed mutagenesis guided by similarities between the zinc fingers of transcription factors Spl (ref. 7) and Krox-20 (ref. 8). Krox-20 is a serum-inducible transcription activator8,9 which is possibly involved in the regulation of hindbrain development10; it contains three zinc fingers similar to those of Spl (refs 7, 8, 11) and binds to a 9-base-pair target sequence which is related to that of Spl (ref. 9). Our results show that each finger spans three nucleotides and indicate two positions in Krox-20 zinc fingers that are important for base-pair selectivity. Modelling with molecular graphics suggests that these residues could bind directly with the bases and that other amino acid–base contacts are also possible. More... »

PAGES

175-178

Journal

TITLE

Nature

ISSUE

6305

VOLUME

349

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/349175a0

    DOI

    http://dx.doi.org/10.1038/349175a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1013911800

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1898772


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