Quantitation of antigen-presenting cell MHC class II/peptide complexes necessary for T-cell stimulation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

C V Harding, E R Unanue

ABSTRACT

The number of specific complexes formed between peptide and the class II major histocompatibility complex (MHC) molecules expressed by an antigen-presenting cell (APC) after exposure to protein antigens is unknown, as is the number that activates T cells. Presentation of foreign peptides by APC takes place when many class II molecules may be occupied by autologous peptides. We have now estimated the number of specific peptide/class II complexes per APC by quantitative immunoprecipitation of I-Ak after pulsing the APC with stimulatory levels of a radioactive immunogenic peptide derived from hen egg-white lysozyme protein. T cells were activated by APC that expressed as few as 210-340 specific peptide/class II complexes (0.1% of the I-Ak molecules). These figures were confirmed using anti-CD3 antibody bound to latex beads as an alternative activating ligand. This low number explains the simultaneous presentation of multiple foreign antigens, even in the face of peptide competition. More... »

PAGES

574-576

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/346574a0

DOI

http://dx.doi.org/10.1038/346574a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043246091

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2115981


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