Dual ion-channel regulation by cyclic GMP and cyclic GMP-dependent protein kinase View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-03

AUTHORS

Douglas B. Light, Jackie D. Corbin, Bruce A. Stanton

ABSTRACT

ATRIAL natriuretic peptide, acting through its second messenger guanosine 3',5'-cyclic monophosphate (cGMP), suppresses Na+ absorption across the renal inner-medullary collecting duct and increases urinary Na+ excretion. Patch clamp studies show that cGMP reduces Na+ absorption by inhibiting an amiloride-sensitive cation channel in the apical membrane. We have now examined, using the patch clamp technique, the molecular mechanisms of cGMP inhibition. Cyclic GMP directly and specifically reduced the probability of a single channel being open (open probability, Po) by 39% (inhibition constant, Ki = 7.6 x 10−7 M) by a phos-phorylation-independent mechanism. Cyclic GMP also inhibited the channel by activating cGMP-dependent protein kinase (cGMP-kinase). Exogenous cGMP-kinase completely inhibited the channel by a phosphorylation-dependent mechanism. Activation of a pertussis toxin-sensitive G protein by GTP-γ-S blocked cGMP-kinase inhibition of the channel. By contrast, cGMP-kinase inhibition of Po was completely reversed by GTP-γ-S. Taken together with the results of a previous study showing that a G protein activates the cation channel, these data indicate that cGMP-kinase and a G protein sequentially regulate the cation channel. Our results show that atrial natriuretic peptide, acting through cGMP, inhibits Na+ absorption across the inner-medullary collecting duct by a dual mechanism, and that cGMP-kinase inhibits the channel by a pathway involving a G protein. More... »

PAGES

336-339

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/344336a0

DOI

http://dx.doi.org/10.1038/344336a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007166174

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1690355


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amiloride", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Atrial Natriuretic Factor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cyclic GMP", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electric Conductivity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanosine 5'-O-(3-Thiotriphosphate)", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Guanosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "In Vitro Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ion Channels", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kidney Medulla", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Magnesium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Kinases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sodium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thionucleotides", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, Ripon College, 54971, Ripon, Wisconsin, USA", 
          "id": "http://www.grid.ac/institutes/grid.262571.5", 
          "name": [
            "Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA", 
            "Department of Biology, Ripon College, 54971, Ripon, Wisconsin, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Light", 
        "givenName": "Douglas B.", 
        "id": "sg:person.01022314070.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022314070.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA", 
          "id": "http://www.grid.ac/institutes/grid.254880.3", 
          "name": [
            "Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Corbin", 
        "givenName": "Jackie D.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA", 
          "id": "http://www.grid.ac/institutes/grid.254880.3", 
          "name": [
            "Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stanton", 
        "givenName": "Bruce A.", 
        "id": "sg:person.010425313662.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010425313662.82"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/317061a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003345839", 
          "https://doi.org/10.1038/317061a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/325442a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027007549", 
          "https://doi.org/10.1038/325442a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/313310a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049661228", 
          "https://doi.org/10.1038/313310a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/321066a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021867825", 
          "https://doi.org/10.1038/321066a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/323812a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034111408", 
          "https://doi.org/10.1038/323812a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1990-03", 
    "datePublishedReg": "1990-03-01", 
    "description": "ATRIAL natriuretic peptide, acting through its second messenger guanosine 3',5'-cyclic monophosphate (cGMP), suppresses Na+ absorption across the renal inner-medullary collecting duct and increases urinary Na+ excretion. Patch clamp studies show that cGMP reduces Na+ absorption by inhibiting an amiloride-sensitive cation channel in the apical membrane. We have now examined, using the patch clamp technique, the molecular mechanisms of cGMP inhibition. Cyclic GMP directly and specifically reduced the probability of a single channel being open (open probability, Po) by 39% (inhibition constant, Ki = 7.6 x 10\u22127 M) by a phos-phorylation-independent mechanism. Cyclic GMP also inhibited the channel by activating cGMP-dependent protein kinase (cGMP-kinase). Exogenous cGMP-kinase completely inhibited the channel by a phosphorylation-dependent mechanism. Activation of a pertussis toxin-sensitive G protein by GTP-\u03b3-S blocked cGMP-kinase inhibition of the channel. By contrast, cGMP-kinase inhibition of Po was completely reversed by GTP-\u03b3-S. Taken together with the results of a previous study showing that a G protein activates the cation channel, these data indicate that cGMP-kinase and a G protein sequentially regulate the cation channel. Our results show that atrial natriuretic peptide, acting through cGMP, inhibits Na+ absorption across the inner-medullary collecting duct by a dual mechanism, and that cGMP-kinase inhibits the channel by a pathway involving a G protein.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/344336a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6264", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "344"
      }
    ], 
    "keywords": [
      "atrial natriuretic peptide", 
      "natriuretic peptide", 
      "cation channels", 
      "cyclic GMP", 
      "G proteins", 
      "pertussis toxin-sensitive G protein", 
      "toxin-sensitive G protein", 
      "patch-clamp technique", 
      "patch-clamp studies", 
      "amiloride-sensitive cation channel", 
      "clamp studies", 
      "clamp technique", 
      "second messenger guanosine", 
      "independent mechanisms", 
      "cGMP-dependent protein kinase", 
      "protein kinase", 
      "cyclic monophosphate", 
      "ion channel regulation", 
      "cGMP kinase", 
      "inhibition", 
      "dual mechanism", 
      "cGMP inhibition", 
      "molecular mechanisms", 
      "cyclic GMP-dependent protein kinase", 
      "cGMP", 
      "GMP-dependent protein kinase", 
      "phosphorylation-dependent mechanism", 
      "duct", 
      "apical membrane", 
      "GMP", 
      "previous studies", 
      "protein", 
      "excretion", 
      "peptides", 
      "kinase", 
      "study", 
      "mechanism", 
      "activation", 
      "GTP", 
      "monophosphate", 
      "pathway", 
      "guanosine", 
      "regulation", 
      "contrast", 
      "results", 
      "PO", 
      "membrane", 
      "absorption", 
      "data", 
      "channels", 
      "single channel", 
      "technique", 
      "probability"
    ], 
    "name": "Dual ion-channel regulation by cyclic GMP and cyclic GMP-dependent protein kinase", 
    "pagination": "336-339", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1007166174"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/344336a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1690355"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/344336a0", 
      "https://app.dimensions.ai/details/publication/pub.1007166174"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:20", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_236.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/344336a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/344336a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/344336a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/344336a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/344336a0'


 

This table displays all metadata directly associated to this object as RDF triples.

219 TRIPLES      21 PREDICATES      101 URIs      88 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/344336a0 schema:about N0c5b7d547085401cb53b176839f846b9
2 N25cd7ed8bc1e4214a5f60094a99193e0
3 N25cf43c5af2f480095c308d6afb163d7
4 N270e46858b10445388d020e3f755c66b
5 N2b9fa90e14454a41b1320115706b62c4
6 N2f34302bb17849388def093dc092ec4d
7 N3612e5ad39b54582b2cad6b7908558ed
8 N46e566753f254aabaa422ffe62780fb4
9 N6aa22108663443a0910905b8c1654646
10 N9958d3b2dfdd4e0b93bc0067cde2d3f2
11 N9aa47dfbd7a84c8da6e9ec46a38c0692
12 Na7d608d2042f4f62a8d8ba26e054ea29
13 Nbf1160b7e9c544a69e79c905bf144288
14 Nc5248b8cd77c4d11b765f53e22627916
15 Nd073bafde54e40b4890c510f3ac0a702
16 Ne88c17d74b894534b6b157ae7ef89143
17 Nfe3554ff83dc4eb79a249966bbee97d6
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author N58443bacf305406aa72a97cbdc285fde
21 schema:citation sg:pub.10.1038/313310a0
22 sg:pub.10.1038/317061a0
23 sg:pub.10.1038/321066a0
24 sg:pub.10.1038/323812a0
25 sg:pub.10.1038/325442a0
26 schema:datePublished 1990-03
27 schema:datePublishedReg 1990-03-01
28 schema:description ATRIAL natriuretic peptide, acting through its second messenger guanosine 3',5'-cyclic monophosphate (cGMP), suppresses Na+ absorption across the renal inner-medullary collecting duct and increases urinary Na+ excretion. Patch clamp studies show that cGMP reduces Na+ absorption by inhibiting an amiloride-sensitive cation channel in the apical membrane. We have now examined, using the patch clamp technique, the molecular mechanisms of cGMP inhibition. Cyclic GMP directly and specifically reduced the probability of a single channel being open (open probability, Po) by 39% (inhibition constant, Ki = 7.6 x 10−7 M) by a phos-phorylation-independent mechanism. Cyclic GMP also inhibited the channel by activating cGMP-dependent protein kinase (cGMP-kinase). Exogenous cGMP-kinase completely inhibited the channel by a phosphorylation-dependent mechanism. Activation of a pertussis toxin-sensitive G protein by GTP-γ-S blocked cGMP-kinase inhibition of the channel. By contrast, cGMP-kinase inhibition of Po was completely reversed by GTP-γ-S. Taken together with the results of a previous study showing that a G protein activates the cation channel, these data indicate that cGMP-kinase and a G protein sequentially regulate the cation channel. Our results show that atrial natriuretic peptide, acting through cGMP, inhibits Na+ absorption across the inner-medullary collecting duct by a dual mechanism, and that cGMP-kinase inhibits the channel by a pathway involving a G protein.
29 schema:genre article
30 schema:isAccessibleForFree false
31 schema:isPartOf Na194048d674d4e50bececd00ce0c0add
32 Na32df61aa4bd4dcda86544a30ba8ae27
33 sg:journal.1018957
34 schema:keywords G proteins
35 GMP
36 GMP-dependent protein kinase
37 GTP
38 PO
39 absorption
40 activation
41 amiloride-sensitive cation channel
42 apical membrane
43 atrial natriuretic peptide
44 cGMP
45 cGMP inhibition
46 cGMP kinase
47 cGMP-dependent protein kinase
48 cation channels
49 channels
50 clamp studies
51 clamp technique
52 contrast
53 cyclic GMP
54 cyclic GMP-dependent protein kinase
55 cyclic monophosphate
56 data
57 dual mechanism
58 duct
59 excretion
60 guanosine
61 independent mechanisms
62 inhibition
63 ion channel regulation
64 kinase
65 mechanism
66 membrane
67 molecular mechanisms
68 monophosphate
69 natriuretic peptide
70 patch-clamp studies
71 patch-clamp technique
72 pathway
73 peptides
74 pertussis toxin-sensitive G protein
75 phosphorylation-dependent mechanism
76 previous studies
77 probability
78 protein
79 protein kinase
80 regulation
81 results
82 second messenger guanosine
83 single channel
84 study
85 technique
86 toxin-sensitive G protein
87 schema:name Dual ion-channel regulation by cyclic GMP and cyclic GMP-dependent protein kinase
88 schema:pagination 336-339
89 schema:productId N308a4df13fb74c69851fd010b4abcb13
90 N885466cd31504d8f916be34f03e44151
91 Ne5c4c8eff39945a3b675822e88288b53
92 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007166174
93 https://doi.org/10.1038/344336a0
94 schema:sdDatePublished 2022-12-01T06:20
95 schema:sdLicense https://scigraph.springernature.com/explorer/license/
96 schema:sdPublisher Ne854ac27ee8c40e0a769a8a0c0341ac5
97 schema:url https://doi.org/10.1038/344336a0
98 sgo:license sg:explorer/license/
99 sgo:sdDataset articles
100 rdf:type schema:ScholarlyArticle
101 N0c5b7d547085401cb53b176839f846b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Cell Line
103 rdf:type schema:DefinedTerm
104 N25cd7ed8bc1e4214a5f60094a99193e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Protein Kinases
106 rdf:type schema:DefinedTerm
107 N25cf43c5af2f480095c308d6afb163d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Amiloride
109 rdf:type schema:DefinedTerm
110 N270e46858b10445388d020e3f755c66b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Rats
112 rdf:type schema:DefinedTerm
113 N2b9fa90e14454a41b1320115706b62c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Thionucleotides
115 rdf:type schema:DefinedTerm
116 N2f34302bb17849388def093dc092ec4d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Magnesium
118 rdf:type schema:DefinedTerm
119 N308a4df13fb74c69851fd010b4abcb13 schema:name doi
120 schema:value 10.1038/344336a0
121 rdf:type schema:PropertyValue
122 N3612e5ad39b54582b2cad6b7908558ed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Animals
124 rdf:type schema:DefinedTerm
125 N46e566753f254aabaa422ffe62780fb4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Guanosine 5'-O-(3-Thiotriphosphate)
127 rdf:type schema:DefinedTerm
128 N57947e0365fa4adbb903d4e1de9a7660 schema:affiliation grid-institutes:grid.254880.3
129 schema:familyName Corbin
130 schema:givenName Jackie D.
131 rdf:type schema:Person
132 N58443bacf305406aa72a97cbdc285fde rdf:first sg:person.01022314070.33
133 rdf:rest Nad0cbf4dba1a48a59cd67bd79a2c968a
134 N6aa22108663443a0910905b8c1654646 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Sodium
136 rdf:type schema:DefinedTerm
137 N7747ec7cf11d46cf87eec27a337b472a rdf:first sg:person.010425313662.82
138 rdf:rest rdf:nil
139 N885466cd31504d8f916be34f03e44151 schema:name dimensions_id
140 schema:value pub.1007166174
141 rdf:type schema:PropertyValue
142 N9958d3b2dfdd4e0b93bc0067cde2d3f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Cell Membrane
144 rdf:type schema:DefinedTerm
145 N9aa47dfbd7a84c8da6e9ec46a38c0692 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Atrial Natriuretic Factor
147 rdf:type schema:DefinedTerm
148 Na194048d674d4e50bececd00ce0c0add schema:volumeNumber 344
149 rdf:type schema:PublicationVolume
150 Na32df61aa4bd4dcda86544a30ba8ae27 schema:issueNumber 6264
151 rdf:type schema:PublicationIssue
152 Na7d608d2042f4f62a8d8ba26e054ea29 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
153 schema:name Ion Channels
154 rdf:type schema:DefinedTerm
155 Nad0cbf4dba1a48a59cd67bd79a2c968a rdf:first N57947e0365fa4adbb903d4e1de9a7660
156 rdf:rest N7747ec7cf11d46cf87eec27a337b472a
157 Nbf1160b7e9c544a69e79c905bf144288 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Guanosine Triphosphate
159 rdf:type schema:DefinedTerm
160 Nc5248b8cd77c4d11b765f53e22627916 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Electric Conductivity
162 rdf:type schema:DefinedTerm
163 Nd073bafde54e40b4890c510f3ac0a702 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Kidney Medulla
165 rdf:type schema:DefinedTerm
166 Ne5c4c8eff39945a3b675822e88288b53 schema:name pubmed_id
167 schema:value 1690355
168 rdf:type schema:PropertyValue
169 Ne854ac27ee8c40e0a769a8a0c0341ac5 schema:name Springer Nature - SN SciGraph project
170 rdf:type schema:Organization
171 Ne88c17d74b894534b6b157ae7ef89143 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Cyclic GMP
173 rdf:type schema:DefinedTerm
174 Nfe3554ff83dc4eb79a249966bbee97d6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
175 schema:name In Vitro Techniques
176 rdf:type schema:DefinedTerm
177 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
178 schema:name Biological Sciences
179 rdf:type schema:DefinedTerm
180 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
181 schema:name Biochemistry and Cell Biology
182 rdf:type schema:DefinedTerm
183 sg:journal.1018957 schema:issn 0028-0836
184 1476-4687
185 schema:name Nature
186 schema:publisher Springer Nature
187 rdf:type schema:Periodical
188 sg:person.01022314070.33 schema:affiliation grid-institutes:grid.262571.5
189 schema:familyName Light
190 schema:givenName Douglas B.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022314070.33
192 rdf:type schema:Person
193 sg:person.010425313662.82 schema:affiliation grid-institutes:grid.254880.3
194 schema:familyName Stanton
195 schema:givenName Bruce A.
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010425313662.82
197 rdf:type schema:Person
198 sg:pub.10.1038/313310a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049661228
199 https://doi.org/10.1038/313310a0
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/317061a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003345839
202 https://doi.org/10.1038/317061a0
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/321066a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021867825
205 https://doi.org/10.1038/321066a0
206 rdf:type schema:CreativeWork
207 sg:pub.10.1038/323812a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034111408
208 https://doi.org/10.1038/323812a0
209 rdf:type schema:CreativeWork
210 sg:pub.10.1038/325442a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027007549
211 https://doi.org/10.1038/325442a0
212 rdf:type schema:CreativeWork
213 grid-institutes:grid.254880.3 schema:alternateName Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA
214 schema:name Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA
215 rdf:type schema:Organization
216 grid-institutes:grid.262571.5 schema:alternateName Department of Biology, Ripon College, 54971, Ripon, Wisconsin, USA
217 schema:name Department of Biology, Ripon College, 54971, Ripon, Wisconsin, USA
218 Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA
219 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...