Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-11

AUTHORS

S J Brill, B Stillman

ABSTRACT

Cell-free replication systems for simian virus 40 (SV40) DNA are taken to be a model for the replication of eukaryotic chromosomes, because only one viral protein is required to supplement the replication proteins provided by a human cell extract. To prove that these cellular proteins function in chromosomal DNA replication we have begun to identify homologous proteins in an organism that can be genetically manipulated. Here we report the identification of yeast replication factor-A (yRF-A) from Saccharomyces cerevisiae and show that it is functionally and structurally related to a human protein that is required for the initiation and elongation of SV40 DNA replication. Yeast RF-A, a multi-subunit phosphoprotein, is similar to the human protein in its chromatographic behaviour, subunit structure and DNA-binding activity. The yeast protein will fully substitute for the human protein in an early stage of the initiation of SV40 DNA replication. Substitution of yRF-A in the complete SV40 replication system, however, results in reduced DNA replication, presumably due to a requirement for species-specific interactions between yeast RF-A and the DNA polymerase complex. More... »

PAGES

92-95

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/342092a0

DOI

http://dx.doi.org/10.1038/342092a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039705736

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2554144


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