A novel genetic system to detect protein–protein interactions View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-07

AUTHORS

Stanley Fields, Ok-kyu Song

ABSTRACT

PROTEIN-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We have generated a novel genetic system to study these interactions by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae. This protein is a transcriptional activator required for the expression of genes encoding enzymes of galactose utilization1. It consists of two separable and functionally essential domains: an N-terminal domain which binds to specific DNA sequences (UASG); and a C-terminal domain containing acidic regions, which is necessary to activate transcription2,3. We have generated a system of two hybrid proteins containing parts of GAL4: the GAL4 DNA-binding domain fused to a protein 'X' and a GAL4 activating region fused to a protein 'Y'. If X and Y can form a protein-protein complex and reconstitute proximity of the GAL4 domains, tran-scription of a gene regulated by UASG occurs. We have tested this system using two yeast proteins that are known to interact—SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection. More... »

PAGES

245-246

Journal

TITLE

Nature

ISSUE

6230

VOLUME

340

Related Patents

  • Methods And Compositions For Identifying Receptor Effectors
  • Antibodies Specific For Htm4
  • Electing A Bait Nucleic Acid Encoding A Bait Protein Active In Post Transincriptional Gene Silencing Or Suppressive Of Ptgs In Plants; Preparing A Cdna Prey Library From A Plant Actively Exhibits Ptgs; Conducting A Yeast Two-Hybrid Assay With The Bait And Prey Nucleic Acids, Yields A True Assay Results
  • Bgl7 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Methods And Compositions For Gene Inactivation
  • Determining The Level Of Activity Of The Enzymatic Reaction Wherein A Change In Said Level Between The Presence And Absence Of Said Candidate Agent Indicates That Said Candidate Agent Is A Modulator Of Said Target Protein Function
  • In Vitro Transcription Of A Dna Sequence
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Enzyme Quantification
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Fast And Exhaustive Method For Selecting A Prey Polypeptide Interacting With A Bait Polypeptide Of Interest: Application To The Construction Of Maps Of Interactors Polypeptides
  • Plants Resistant To Pathogens And Methods For Production Thereof
  • In Vitro Evolution In Microfluidic Systems
  • Tumor Necrosis Factor Receptor-Associated Factors
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Method Of Treatment Of Leukemia With Anti-Il1rap Antibodies
  • A Method For Increasing The Concentration Of A Nucleic Acid Molecule
  • Methods And Compositions For Gene Inactivation
  • Reverse Two-Hybrid Method
  • In Vitro Evolution In Microfluidic Systems
  • In Vitro Evolution In Microfluidic Systems
  • Polypeptide Comprising An Amino Acid Sequence And Having Endoglucanase Activity; Detergent With Building Agent, A Bleaching Agent, A Caking Inhibitor
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Hormonally Up-Regulated, Neu-Tumor-Associated Kinase
  • Detection Of Protein To Protein Interactions
  • Random Peptide Library
  • Nucleotide Sequences Coding Polypeptide For Use In The Treatment Of Alzheimer's Diseases
  • Genetic Mapping; Dna Libraries; Genetic Engineered Yeast Cells
  • Population Control Using Engineered Translocations
  • A Target Receptor Protein Whose Signal Transduction Activity Can Be Modulated By Interaction With An Extracellular Signal, And An Expressible Recombinant Gene Encoding An Exogenous Test Polypeptide From A Polypeptide Library; Rapid, Reliable
  • Methods Of Detecting A Solid Tumor With Anti-Il1rap Antibodies
  • Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Yeast Cells Engineered To Produce Pheromone System Protein Surrogates, And Uses Therefor
  • Digital Analyte Analysis
  • Interaction Trap System For Isolating Novel Proteins
  • In Vitro Methods Of Producing And Identifying Immunoglobulin Molecules In Eukaryotic Cells
  • Anti-Bacterial Methods And Materials
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Methods For Inhibiting The Growth Of Cancer Cells Expressing Psca
  • Assay For Tumor Necrosis Factor Receptor-Associated Factors
  • Regulated Apoptosis
  • Vigilant Cells
  • Using Enzymes
  • Gene Expression Modulation System Comprising Ecdysone Receptor Ligand Binding Domain For Use In Gene Therapy And High Throughput Assay; Pesticides; Insecticides
  • Transforming Cells With Gene Expression Vector; Genetic Engineering
  • Multiple Inducible Gene Regulation System
  • Β-Amyloid Peptide-Binding Proteins And Polynucleotides Encoding The Same
  • Humanized Collagen Antibodies And Related Methods
  • Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Killing Genetically Engineered Cells
  • Screening Test Compounds For The Ability To Decrease Or Increase Protein Activity; Contacting Compound With Mixture Of Mammal Inhibitory Protein And Two Other Peptides, Measuring Amount Of Inhibitor Bound To Polypeptides
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Yeast Cells Having Mutations In Stp22 And Uses Therefor
  • Chlorotoxin Polypeptides And Conjugates And Uses Thereof
  • Regulated Transcription Of Targeted Genes And Other Biological Events
  • Detection Of Molecular Interactions By Β-Lactamase Reporter Fragment Complementation
  • Method Of Making A Paired Tag Library For Nucleic Acid Sequencing
  • For Drug Screening; Can Be Used In A High Throughput System
  • Vectors For Differential Expression
  • Methods And Compositions For Modulating Cardiac Contractility
  • Combination Of Selection Steps Used To Detect Clones That Express Interacting Molecules From False Positive Clones.
  • Treatment And Prevention Of Cancer With Hmgb1 Antagonists
  • Eukaryotic Cell Comprising Expression Vectors Coding Chimeric Protein; For The Efficient And Accurate Treatment Of Defects With Gene Therapy
  • Tia-1 Binding Proteins And Isolated Complementary Dna Encoding The Same
  • Multiple Inducible Gene Regulation System
  • Bgl7 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Inhibitors Of Usp1 Deubiquitinating Enzyme Complex
  • Chlorotoxin Polypeptides And Conjugates And Uses Thereof
  • Compounds And Methods For Treating Cancer
  • Isolation Of Proteins Involved In Posttranscriptional Gene Silencing And Methods Of Use
  • Cellulases; Beta-Glucosidases; Hydrolases; Conversion Of Cellulose To Sugars And/Or Ethanol; Laundry Detergents
  • Detection Of Molecular Interactions By Reporter Subunit Complementation
  • Methods And Compositions For In Vivo Non-Covalent Linking
  • Mammalian Dna Mismatch Repair Genes Mlh1 And Pms1
  • Monoclonal Anti-Idiotypic Antibody; Antitumor Agents; Anticarcinogenic Agents; Antiproliferative Agents; Immunotherapy; Hormone Antagonist Therapy
  • Methods And Compositions For Gene Inactivation
  • Cdna Sequences Of Two Interacting Fancip2 And Fancip3 Of The Fanconi Anemia Complementation Group A Protein
  • Bgl7 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Modulation Of Mesenchymal And Metastatic Cell Growth
  • Detecting Modulators Of Protein Interaction; Providing Genetically Engineered Prokaryotic Cells, Incubation Of Cells With Modulators And Monitoring Expression Reporter Genes
  • Modulation Of Stem Cells Using Zinc Finger Proteins
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Zinc Finger Proteins Modulate Expression Of Pigment Epithelium-Derived Factor (Pedf) Gene; Use In Treatment Of Retinopathies And Macular Degeneration
  • Determining Sensitivity Of Two Distinct Proteins To Each Other In Cells That Are Mating By Detecting Expression Of Reporter Genes
  • Β3 Integrin Subunit Specific Polypeptides, Cdnas Which Encode These Polypeptides And Methods Of Producing These Polypeptides
  • Detection Of Molecular Interactions By Reporter Subunit Complementation
  • Fluorocarbon Emulsion Stabilizing Surfactants
  • Biologically Active Molecular Targets That Function As A Dominant Negative Modulator Of Tir Signal Transduction; Interleukin-1 Receptor-Associated Kinase 1c; Polynucleotides, Polypeptides, Expression Vectors And Host Cells; Screening Assays, Diagnosis
  • Nucleotide Sequences Coding Enzymatic Polypeptide For Use In The Treatment Of Psychological Disorders
  • Digital Analyte Analysis
  • Distinguishing Rare Variations In A Nucleic Acid Sequence From A Sample
  • Compositions And Methods For Molecular Labeling
  • Using Library Of Recombinant Yeast Cells, Each With Gene Encoding A Heterologous Orphan Receptor Expressed On Cell Membrane And Involved In Signal Transduction Of Extracellular Signal And Gene Expressing Test Polypeptide
  • Plasmids Which Express Heterologous Genes In Both Plant And Animal Cells; Fusion Proteins; Gene Therapy; High Throughput Assays; Genomics; Host Cells; Animal Models
  • Methods For Producing A Paired Tag From A Nucleic Acid Sequence And Methods Of Use Thereof
  • Method For Inducing Cell Death In Acute Lymphoblastic Leukemic System Cells
  • Bgl5 Β-Glucosidase And Nucleic Acids Encoding The Same
  • Determining Protein-Protein Interactions By Counterselection Of Two Test Proteins Expressed In Two Separate Nucleic Acid Libraries And Measuring Level Of Expression Of Reporter Gene Operably Linked To A Dna-Binding-Protein Recognition Sites
  • Methods Of Generating Variant Proteins With Increased Host String Content
  • Ecdysone Receptor/Invertebrate Retinoid X Receptor-Based Inducible Gene Expression System
  • System To Detect Protein-Rna Interactions
  • Detecting Associations Between Two Fusion Proteins In Prokaryotic Cells Using Reporter Gene Linked To Regulatory Sequences Which Includes Binging Site
  • Cbh1 Homologs And Varian Cbh1 Cellulase
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Purified And Isolated Polynucleotide Which Encodes Functional Inhibitor Of Myod Family Protein
  • Selection By Compartmentalised Screening
  • Methods And Compositions For Identifying Receptor Effectors
  • Targeting Pax2 For The Induction Of Defb1-Mediated Tumor Immunity And Cancer Therapy
  • Cytoskeletal Filament Proteins, Tubulin And Actin; Atpases; Drug Screening; Motor Proteins; High Throughput Assay
  • Method For Producing Polymers Having A Preselected Activity
  • Method Of Treating A Solid Tumor With Il1rap Antibodies
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • Regulated Transcription Of Targeted Genes And Other Biological Events
  • Genetically Engineered Polypeptide For Treatment Of Cardiovascular And Neurodegenerative Disorders; Anticarcinogenic, Antitumor, And Antiproliferative Agents; Non-Insulin Dependent Diabetes; Neuropathic Pain, Dyslipidemia, Obesity, And Retinopathy
  • Kit For Detecting The Level Of Cyclin-Dependent Kinase Inhibitor P16 Gene Expression
  • Humanized Collagen Antibodies And Related Methods
  • Two Hybrid Assay That Detects Hiv-1 Reverse Transcriptase Dimerization
  • Variant Enzymes
  • Psca Antibodies And Hybridomas Producing Them
  • Reporter Signals For Use As Diagnostic Tools In Genetic Engineering
  • Drosophila G Protein Coupled Receptors, Nucleic Acids, And Methods Related To The Same
  • Purified Human Cellular Component That Binds To Translation Factor, Elf-4e, And Causes Modulation Of Translation In Cell In Response To Hormone; Antidiabetic Agents; Treatment Of Glandular Disorders Such As Addison's Disease
  • Modulating Apoptosis Of A Cell By Administering An Agent Comprising A Bh1 Or Bh2 Domain Capable Of Promoting Or Inhibiting Formation Of A Complex Of Bcl-2:Blc-2; Bcl-Xl:Bcl-Xl; Bax:Bax; And/Or Bcl-Xl:Bax
  • In Vitro Method For Predicting The Evolutionary Response Of A Protein To A Drug Targeted Thereagainst
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • Regulated Apoptosis
  • Producing Vector Libraries Capable Of Expressing Variable Heavy And Light Chain Polypeptides By Synthesizing Variable Chains, Amplify And Join The Variable Heavy Chain To The Light Chain Into Expression Vectors And Screen The Libraries
  • Separately Amplifying Two Different Nucleic Acid Molecules With Two Sets Of Different Primers, Mixing The Products And Amplifying The Hybridized Molecule Creating A Hybrid Molecule For Creating Libraries
  • Nucleotide Sequence Coding Human Maintenance Protein; For The Detection And Treatment Of Tumors; For The Detection Of Oncogenes
  • Cell Culture Compositions
  • Prevention, Therapy Of Central Nervous System Disorders; Neurodegradation; Exposure Protein To Drug ; Measuring Binding; Calibration
  • Contacting Virulence Protein With Chemical Compound, Then Identifying Compounds Which Bind Or Interacts With Protein; For Bactericides Against Staphylococcus Aureus
  • Cytotoxicity-Based Genetic Selection System (Toxsel)
  • In Vitro Evolution In Microfluidic Systems
  • Aegyptin And Uses Thereof
  • Tetracycline-Regulated Transcriptional Activator Fusion Proteins
  • Isolating Nucleic Acid Encoding A Plant Polypeptide For Use In Generating Disease Resistant Plants; Obtain Library, Incubate With Probe, Recover Clone, Transform Plant, Propagate, Evaluate For Disease Resistance
  • Leafthopper Ecdysone Receptor Nucleic Acids, Polypeptides, And Uses Thereof
  • Ras P21-Interacting Protein (Rgl) And Its Ras Interacting Domain (Rid)
  • Anti-Angiogenic Methods And Compositions
  • Cloning System And Method For Identifying Interaction Partners In Protein Complexes
  • Bgl7 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Comprises Membrane Protein For Use In The Diagnosis, Prevention And Treatment Of Tumor Disorders
  • Systems For Handling Microfludic Droplets
  • Diagnosis Of Risk For Unwanted Cell Proliferation Or Differentiation By Determining Phenotype Of Cell Sample By Detecting Abberant Modification Of Gene Encoding A Cyclin Dependent Kinase Inhibitory Protein Or Its Mis-Expression
  • Method Of Treatment For Leukemia Using An Anti-Il1rap Antibody
  • Variant Enzymes
  • Poly(Adp-Ribose) Polymerase Genes
  • Comprehensive Analysis Of Transcription Control Factor Complexes In A Mouse Brain Cdna Library With C-Jun As A Bait By Using The Cotranslation Selection And Screening Of In Vitro Virus (Ivv) And The C-Terminal Labeling Method
  • Identification Of The P21waf1-Pcna Interaction Site And Therapeutic Applications Thereof
  • Based On Alterations In Zeb Expression Levels And Subcellular Localization.
  • For Inducible Dimerization/Oligomerization Of Intracellular Proteins; For Gene Therapy
  • Dna Molecules Encoding Rip-1 Protein Which Binds To The Human Immunodeficiency Virus Viral Protein R; Viricides And Prophylactic Agents
  • As Diagnostic And Therapeutic Assays And Reagents For Detecting And Therapy Of Proliferative Disorders Arising From, For Example, Tumorigenic Transformation Of Cells, Or Other Hyperplastic Or Neoplastic Transformation Processes
  • Small Molecule Inhibitors Of Usp1 Deubiquitinating Enzyme Activity
  • Adenovirus Vector Containing Nucleotide Sequence Coding Polypeptide Which Binds And Inhibits Cyclin Kinase; Screening For Agonist Or Antagonist To Cell Propagation; Develop Immunogen; Antitumor Agents; Diagnostic Model For Disease
  • Tata-Binding Protein Associated Factors, Nucleic Acids Encoding Tafs, And Methods Of Use
  • Aegyptin And Uses Thereof
  • Cellular Receptor For Hiv-1 Vpr Essential For G2/M Phase Transition Of The Cell Cycle
  • Reconstituted Polypeptides
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Totally Synthetic Affinity Reagents
  • Nucleic Acid Adaptors And Uses Thereof
  • Detection Of Protein To Protein Interactions
  • The Present Invention Relates, In General, To Novel Tetratricopeptide Repeat (Tpr)-Containing Genes, Tpr1 And Tpr2.
  • Targeting Pax2 For The Induction Of Defb1-Mediated Tumor Immunity And Cancer Therapy
  • Cdna Sequences Of Two Interacting Fancip2 And Fancip3 Of The Fanconi Anemia Complementation Group A Protein
  • Two-Hybrid Based Screen To Identify Disruptive Residues At Multiple Protein Interfaces
  • Nucleotide Sequences Coding Binding Polypeptide; For Monitorin Interleukin Induced Signal Transduction; For Diagnosis And Treatment Of Interleukin Defects
  • Drosophila G Protein Coupled Receptors, Nucleic Acids, And Methods Related To The Same
  • Egvi Endoglucanase And Nucleic Acids Encoding The Same
  • Fluorocarbon Emulsion Stabilizing Surfactants
  • Selection By Compartmentalised Screening
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Using Transgene Expression Of Enzyme Inhibitors And Associated Factors To Control Morphological Characteristics Such As Size And Shape Of Crop Plants
  • Comprises Antibody Which Associates With Fibronectin Type Iii (Fn3) Polypeptide For Treatment Of Nuclear Receptor-Related Diseases
  • Method Of Making A Paired Tag Library For Nucleic Acid Sequencing
  • Engineered Cleavage Half-Domains
  • Detecting Prostate Stem Cell Antigen Proteins By Contacting Sample With Monoclonal Antibody In An Immunoassay
  • Nucleotide And Amino Acid Sequence Of Protein Involved In Telomeres; Anticarcinogenic Agents; Treatment Of Age-Related Diseases
  • Antibody For Use In The Treatment Of Tumors
  • Anti-Htra1 Antibodies And Methods Of Use Thereof
  • Introducing Into The Cell A Nucleic Acid Molecule Encoding A Tetracycline-Controllable Transactivator (Tta) Comprising Tet Repressor Operably Linked To A Polypeptide Which Or Activates Transcription In Eucaryotic Cell; Modulating
  • Interleukin-8 Homologous Polypeptides And Therapeutic Uses Thereof
  • Ifn Receptor 1 Binding Proteins, Dna Encoding Them, And Methods Of Modulating Cellular Response To Interferons
  • Method For Producing Polymers Having A Preselected Activity
  • Labelling And Selection Of Molecules
  • Antibody Which Binds To An Epitope On A Protein Associated With Viral Replication And Cell Cycle Control; For The Treatment Of Viral Diseases And Cancers; Antitumor Agents; Anticarcinogenic Agents
  • Orphan Nuclear Receptor
  • Digital Analyte Analysis
  • Detection Cloning; Isolation Hybrids; Using Labels; Genetic Engineering
  • Chlorotoxin Variants, Conjugates, And Methods For Their Use
  • Egvii Endoglucanase And Nucleic Acids Encoding The Same
  • Anticancer Agents
  • Reconstituted Polypeptides
  • Compositions And Methods For Identifying And Testing Tgf-Β Pathway Agonists And Antagonists
  • Methods And Compositions For Gene Inactivation
  • Detection Of Molecular Interactions By Reporter Subunit Complementation
  • Identifying Modulators Of G-Protein Coupled Receptors In Yeast Pheromone Response Pathways For Treating And Preventing Bacterial, Fungal, Parasitic And Viral Infection
  • Bgl6 Β-Glucosidase And Nucleic Acids Encoding The Same
  • Prevention And Treatment Of Cast Nephropathy
  • Anti-Htra1 Antibodies And Methods Of Use
  • Factors Which Modify Gene Transcription And Methods Of Use Therefor
  • Tumor Necrosis Factor Receptor-Associated Factors
  • In Vitro Evolution In Microfluidic Systems
  • Antimicrobial Methods And Materials
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Cell Free Methods For Detecting Protein-Ligand Binding
  • Incubating Cyclophilin Affinity Fusion Protein; Complexing; Binding To Affinity Medium; Comparing To Control
  • Cell Death Regulator
  • Bgl3 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Cyclin Dependent Kinase Mutants, And Diagnostic And Therapeutic Uses Related Thereto
  • Screening Method For Candidate Drugs
  • Digital Analyte Analysis
  • Cytotoxic Immunoglobulin
  • Polypeptide For The Use In The Prevention On Viral Infection; Viricides
  • Synthetic Immunoglobulin Domains With Binding Properties Engineered In Regions Of The Molecule Different From The Complementarity Determining Regions
  • Methods For Producing A Paired Tag From A Nucleic Acid Sequence And Methods Of Use Thereof
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Engineered Cleavage Half-Domains
  • Antitumor Agents; Anticancer Agents; Antibody Binding
  • Cbh1 Homologs And Variant Cbh1 Cellulases
  • Phosphatase Modulator
  • Ecdysone Receptor-Based Inducible Gene Expression System
  • In Vitro Methods Of Producing And Identifying Immunoglobulin Molecules In Eukaryotic Cells
  • Yeast Cells Expressing Modified G Proteins And Methods Of Use Therefor
  • Totally Synthetic Affinity Reagents
  • Inhibitors Of Memapsin 2 And Use Thereof
  • Mk2 Interacting Proteins
  • Comprising Heterologous Gene Encoding Farnesyl Transferase And Second Heterologous Gene Encoding Peptide Which Modulates The Interaction Of Enzyme; For Screening Of Peptide Libraries For Drugs
  • Using Reporter Protein Expression As Diagnostic Indicator Preferential Protein Activity; Bioassay
  • Substitution Mutant Receptors And Their Use In An Ecdysone Receptor-Based Inducible Gene Expression System
  • Prostate Stem Cell Antigen (Psca) Tumor Antigen For Use In Diagnosis, Prevention And Treatment Of Prostate Cancers
  • Bgl7 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Acyl Amino Acids
  • Synthetic Transcription Factor And Uses Thereof
  • Anti-Htra1 Antibodies And Methods Of Use Thereof
  • Specified Complementary Determining Region (Crd) Amino Acid Sequences; Specific Binding Activity For A Cryptic Collagen Epitope; Angiogenesis Inhibition, Antimetastasis, And Tumor-Targeted Therapy Uses
  • Using A Dna
  • Antibody Libraries
  • Methods And Compositions For Targeted Gene Modification
  • Antibody To Herpes Virus Entry Receptor Protein
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Comprises Attenuated Gram Negative Bacterial Mutant Genes; For Detecting Genes Responsible For Virulence Of Pasteurellaceae Bacteria
  • Methods Of Identifying Agents That Affect Cleavage Of Amyloid-Β Precursor Protein
  • Secreted Proteins
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • Lafora's Disease Gene
  • Isolated Nucleic Acids; Complexing With Antibodies; Detector
  • Nucleotide Sequence Of Protein Involved In Embryogenesis And In Adult Hematopoietic Cells
  • Targeted Epigenetic Therapy For Inherited Aortic Aneurysm Condition
  • Transgenic Yeast Cells For Monitoring Interactions Between Random Peptides And Polypeptides Asociated With The Post-Translational Modification, Transport And Response Of Yeast Pheromones; Drug Screening
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • Treatment Of Metastatic Tumors
  • Psca: Prostate Stem Cell Antigen And Uses Thereof
  • Method Of Modulating Gene Expression Using An Ecdysone Receptor-Based Inducible Gene Expression System
  • High Throughput Generation And Affinity Maturation Of Humanized Antibody
  • Sandwich Assays In Droplets
  • Tia-1 Binding Proteins And Isolated Complementary Dna Encoding The Same
  • Plant Protein For Use As Tool In Cintrolling Gene Expression Plants
  • Egvii Endoglucanase And Nucleic Acids Encoding The Same
  • Using Enzyme As Catalyst
  • Reversed Mammalian Protein-Protein Interaction Trap
  • Selectively Inhibit Memapsin 2 Beta -Secretase Activity Relative To Memapsin 1 Beta -Secretase Activity; Alzheimer's Disease
  • Reverse Two-Hybrid Method
  • Cell Surface Proteins And Use Thereof As Indicators Of Activation Of Cellular Signal Transduction Pathways
  • Antibody Libraries
  • Selection Of Compartmentalized Screening Method
  • Digital Analyte Analysis
  • Screening Method
  • Administering Peptides Or Peptide Fragments
  • Polynucleotide Coding Binding Polypeptide; For Use In Prevention And Treatment Of Viral Diseases And Development Of Viricides
  • Isolating Polypeptide Genes Coding For A Receptor Having Catalytic Acitivity That Is Capable Of Binding Ligands Having Immunological Activity
  • Human Receptors And Membrane-Associated Proteins (Remap); Use In Diagnosis Or Treatment Of Cell Proliferative, Autoimmune/Inflammatory, Neurological, Metabolic, Developmental, And Endocrine Disorders
  • Anticancer, Antitumor Agents; Monoclonal Antibodies; Genetic Engineered Protein
  • Bgl6 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Method And Systems For Investigating The Ubiquitin-Proteasome System
  • Comprises Receptor-Like Protein Tyrosine Phosphatase Polypeptides (Dep-1) For Use As Antiinflammatory/Anticarcinogenic Agents
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • High Throughput Generation And Affinity Maturation Of Humanized Antibody
  • Methods Of Assessing Wolframin Protein Activity
  • Regulated Transcription Of Targeted Genes And Other Biological Events
  • Amino Acid Sequences
  • Means And Methods For Detecting Protein-Peptide Interactions
  • Methods And Compounds For Modulating Male Fertility
  • Nucleotide Sequences Coding Signal Transduction Components In Durable And Broad-Range Resistance Strategies Based On Plant Defence
  • Methods Of Generating Variant Antibodies With Increased Host String Content
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Methods For Treating Cancer Using Psca Antibodies And Fragments Thereof
  • Microfluidic Systems And Methods For Reducing The Exchange Of Molecules Between Droplets
  • Involves Selection Of A Gene Of Therapeutic Interest And Determination Of Regulatory Dna Sequences ; Assessing Potential Pharmacological Effects And/Or Side Effects Of An Agent Based On The Distribution And Properties Of Chromatin
  • Yeast Cells Engineered To Produce Pheromone System Protein Surrogates, And Uses Therefor
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Antibody Libraries
  • Bgl3 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Nuclear Cyclophilin
  • Cell-Cycle Regulatory Proteins, And Uses Related Thereto
  • Cell Comprising Three Reporter Genes Each Linked To A Promoter And A Fusion Gene That Encodes A Polypeptide Fused To A Dna Binding Moiety, And The Reporter Genes Are Linked To An Exogenous Promoter; Use For Identifying Molecular Interactions (E.G., Protein/Protein, Protein/Dna); Counterselectable Markers
  • Lafora's Disease Gene
  • Predicting The Function Of Proteins
  • 3-Allyl-Rapamycin, 3-Methallyl-Rapamycin; C3-Substituted Rapamycin Rapalogs; Multimerizing Chimeric Proteins In Engineered Cells; Reduced Immunosuppressive Effect; Gene Therapy; Form A Tripartite Complex With Fkbp12 And A Genetically Engineered Frb Domain, Or Fusion Proteins Having Fkbp And Frb Domains
  • Interaction Of Proteins Involved In A Cell Death Pathway
  • Isolating Nucleic Acid Encoding A Plant Polypeptide For Use In Generating Disease Resistant Plants; Obtain Library, Incubate With Probe, Recover Clone, Transform Plant, Propagate, Evaluate For Disease Resistance
  • Isolate Nucleic Acids Containing Immunolgical Portions, Prepare Primers For Cloning Polynucleotides Segments Having The Variable Heavy And Light Chain Regions; Prepare And Express The Gene Libraries, Screening For Target Sequences
  • Pdes And Uses Thereof
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • A Genetic Construct Encoding A Chimeric Protein Having An Activatable Apoptosis-Domain To Complex With A Nonprotein, Membrane-Permeable Ligand Which Then Binds To Another Protein Thereby Activating The Apoptosis-Inducing Domain
  • Molecule Library Constructed On The Basis Of Backbone Structure Of Microprotein
  • Htm4 Methods Of Treatment And Assays, Agonists And Antagonists
  • Display Of Binding Agents
  • Anti-Angiogenic Methods And Compositions
  • Screening For Modulators Of Mammalian Cell Cycle Regulatory Proteins; Administering A Compound To A Cell Line And Analyzing The Expression Of A Reporter Gene In The Cell Line, Thereby Determining Whether The Compound Affects Normal
  • Βiv-Spectrin-Polypeptides And Nucleic Acids Encoding Same
  • Brain Cyclic Nucleotide Gated Ion Channel And Uses Thereof
  • Method And Compositions For Prevention And Treatment Of Malaria Infections
  • Pharmacogenomics And Identification Of Drug Targets By Reconstruction Of Signal Transduction Pathways Based On Sequences Of Accessible Regions
  • Psca: Prostate Stem Cell Antigen And Uses Thereof
  • Secreted Proteins
  • Using Bioreactor To Expression Recombinant Proteins Which Bind And/Or Modulate Cellular Activities; Dna Binding Domain And Transcription Activators
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • Bgl6 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Databases Of Regulatory Sequences; Methods Of Making And Using Same
  • Compounds And Methods For Treating Cancer
  • Anti-Angiogenic Methods And Compositions
  • Methods For Detecting Interaction Among Several Proteins
  • Reconstituted Polypeptides
  • Nucleic Acid Encoding Sm1 Resistance To Orange Wheat Blossom Midge And Method Of Use
  • Identification Of Genes Responsible For The Virulence Of Staphylococcus Bacteria, Which Allows For Identification Of New Anti-Bacterial Agents And Mutants That Can Be Used As Vaccines
  • Screening Method For Candidate Drugs
  • S-Layer Protein 2d Lattice Coupled Detergent-Free Gpcr Bioelectronic Interfaces, Devices, And Methods For The Use Thereof
  • Plasmids Which Express Heterologous Genes In Both Plant And Animal Cells; Fusion Proteins; Gene Therapy; High Throughput Assays; Genomics; Host Cells; Animal Models
  • Egviii Endoglucanase And Nucleic Acids Encoding The Same
  • Method For Producing Polymers Having A Preselected Activity
  • Mixture Of Cells Which Express Heterologous G Protein-Coupled Receptor In Membrane; Library Of Heterologous Polypeptides Transported To Specific Location For Extracellular Interaction With Receptor; Drug Screening, Genetic Engineering; Improved Signal-To-Noise Ratio
  • Methods And Compositions For The Treatment Of Fabry Disease
  • Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Synthetic Immunoglobulin Domains With Binding Properties Engineered In Regions Of The Molecule Different From The Complementarity Determining Regions
  • Utilizes Reporter Oligonucleotides As Biochemical Barcodes For Detecting Multiple Protein Structures Or Other Target Non-Nucleic Acid Analytes In One Solution
  • Biomarker Of Asbestos Exposure And Mesothelioma
  • Nucleotide Sequences Coding Enzymatic Polypeptide For Use In The Preparation Of Bread Doughs
  • Cytotoxic Immunoglobulin
  • Detecting Expression Of A Reporter Gene As A Measure Of Protein Interaction Between Mated Cells
  • Sample Multiplexing
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • A Computational Method System, And Computer Program Are Provided For Inferring Functional Links From Genome Sequences.
  • Zinc Finger Proteins And Method For Inactivating A Dhfr Gene In A Chinese Hamster Ovary Cell
  • Site-Specific Enzymes And Methods Of Use
  • Digital Analyte Analysis
  • Kit For Evaluating The Efficacy Of A Compound Against A Biocompatible Mutant Or Wild-Type Form Of A Enzymatic Protein
  • Host Cell Containing Detectable Gene Which Expresses A Detectable Protein When The Detectable Gene Is Activated By An Amino Acid Sequence Including A Transcriptional Activation Domain
  • Variant Enzymes
  • An Essential Element In Signal Transduction Pathways Which Control Fundamental Cellular Processes Including Growth And Differentiation, Cell Cycle Progression, And Cytoskeletal Function
  • Methods Of Inhibiting Or Enhancing The Tgfβ-Smad Signaling Pathway
  • Ecdysone Receptor-Based Inducible Gene Expression System
  • Optical Sorting Method
  • Leafhopper Ecdysone Receptor Nucleic Acids, Polypeptides, And Uses Thereof
  • Use Of Endoglucanase 6 In Saccharification Of Biomass; Fermenation To Bioethanol; Biofuels
  • G Protein-Coupled Receptor-Like Receptors And Modulators Thereof
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Manipulating Droplet Size
  • Chimeric Retroviral Gag Genes And Screening Assays
  • Prostate Specific Antigen; Anticancer Agents
  • Collection Of Prokaryotic Dna For Two Hybrid Systems Helicobacter Pylori Protein-Protein Interactions And Application Thereof
  • Compartmentalised Combinatorial Chemistry By Microfluidic Control
  • Molecule Library Constructed On The Basis Of Backbone Structure Of Microprotein
  • Cellulase Compositions For Use In Household Detergents, Stonewashing Compositions Or Laundry Detergents; Surfactant Resistance, Heat Resistance, Increased Or Decreased Cellulolytic Capacity, And/Or High Level Expression In Vivo
  • Nucleotide Sequences Coding Genetically Engineered Zinc Finger Protein For Use In Treatment And Prevention Of Cell Proliferative Disorders
  • Synthetic Ligation Reassembly In Directed Evolution
  • Libraries Of Regulatory Sequences, Methods Of Making And Using Same
  • Compositions And Assays Utilizing Adp Or Phosphate For Detecting Protein Modulators
  • Murine And Human Box-Dependent Myc-Interacting Protein (Bin1) And Uses Therefor
  • Substitution Mutant Receptors And Their Use In An Ecdysone Receptor-Based Inducible Gene Expression System
  • Chlorotoxin Polypeptides And Conjugates And Uses Thereof
  • Compositions And Methods For Isolating Ligand Binding Polypeptides For A User-Specified Ligand, And For Isolating Small Molecule Ligands For A User-Specified Target Polypeptide Using An Improved Class Of Hybrid Ligand Compounds
  • Antibody Which Specifically Binds To A Oncogene Modulating Peptide; For The Diagnosis And Treatment Of Breast, Prostate, Or Liver Cancer; Anticarcinogenic Agents; Antitumor Agents
  • G Protein-Coupled Receptor-Like Receptors And Modulators Thereof
  • Method For Screening Compounds Inhibiting Signal Transduction Through Inflammatory Cytokines
  • Generation Of Acyl Amino Acids
  • Antibodies To Human Β-Adrenergic Receptor Kinase
  • In Vitro Evolution In Microfluidic Systems
  • Site-Specific Enzymes And Methods Of Use
  • In Vitro Evolution In Microfluidic Systems
  • Kinase Substrate Sensor
  • Site-Directed Mutagensis; Host Cells For Diagnosis/Treatment Of Alzheimer's Disease
  • Β-Glucosidase 5 (Bgl5) Compositions
  • Expressed From Human Cdna
  • Compartmentalised Combinatorial Chemistry By Microfluidic Control
  • Hormonally Up-Regulated, Neu-Tumor-Associated Kinase
  • Compartmentalized Screening By Microfluidic Control
  • Density Enhanced Phosphatase-1 (Dep-1); For Drug Screening Agents For Altering Signal Transduction In Cells; Genetic Engineering
  • Small Molecule Inhibitors Of Usp1 Deubiquitinating Enzyme Activity
  • Cell-Free Synthesis And Isolation Of Novel Genes And Polypeptides
  • Antibodies To The Cell Cycle Regulatory Protein P16
  • Human Ion Channel Proteins
  • Totally Synthetic Affinity Reagents
  • Digital Analyte Analysis
  • A Compound Comprising Two Or More Multimerized Ligands Each Independently Selected From The Group Consisting Of Fk506, Fk520 And Cyclosporin, Or Macrocyclic Derivatives Thereof.
  • Oligomerizes Two Or More Chimeric Protein Molecules To Form A Ligand Cross-Linked Protein Complex
  • Murine And Human Box-Dependent Myc-Interacting Protein (Bin1)
  • Detecting Cells With Cell Proliferation Defects; Detect Mutation In Cyclin Dependent Kinase Of Cell Sample,
  • Receptor Binding Assay For A Compound That Induces Cytochrome P-450 Monooxygenase 3a4 Gene Expression; Drug Screening
  • Factors Which Modify Gene Transcription And Methods Of Use Therefor
  • Adjusting Preferental Particles To Generate Signal; Mix Ligand And Marker, Enzyme, Incubate, Allow Binding, Detect Signal
  • Method Of Treatment Of A Solid Tumor With Interleukin-1 Accessory Protein Antibody
  • Targeting Pax2 For The Induction Of Defb1-Mediated Tumor Immunity And Cancer Therapy
  • Genetically Engineered Plant; For The Control Of Gene Expression In Cells; For Treatment Of Arthritis And Inflammation; Wound Healing; For Treatment Of Cancer; Gene Therapy
  • Chaperonin 10 Variants
  • Egvii Endoglucanase And Nucleic Acids Encoding The Same
  • Chlorotoxin Variants, Conjugates, And Methods For Their Use
  • High Throughput Protein Production Screening
  • Receptor-Based Interaction Trap
  • Method For Detecting And Quantifying Latent Retroviral Rna Species
  • Composition And Methods Relating To Dna Mismatch Repair Genes
  • Modulation Of Stem Cells Using Zinc Finger Proteins
  • Screening For Modulators Of Preferential Enzyme Activity; Incubating Cells With Modulators And Detecting Expression Of Reporter Molecules, Amplification Of Expression Indicates An Inhibitor, Decreased Expression Indicates An Activator
  • Tight Control Of Gene Expression In Eucaryotic Cells By Tetracycline-Responsive Promoters
  • In Vitro Evolution In Microfluidic Systems
  • Manipulation Of Fluids, Fluid Components And Reactions In Microfluidic Systems
  • Modulation Of Stem Cells Using Zinc Finger Proteins
  • Detecting Protein Binding; Provide Genetically Engineered Cell, Measure Protein Binding By Preferential Activation Of Reporter Molecules
  • Bgl6 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Hcv Core Protein Binding Agents For Treatment Of Hepatitis C Virus Infection
  • Synthetic Immunoglobulin Domains With Binding Properties Engineered In Regions Of The Molecule Different From The Complementarity Determining Regions
  • Genetically Engineered Cell Coding Chimeric Protein Associated With Oligomerization; For Gene Therapy
  • Murine And Human C-Myc Interacting Protein
  • Prokaryotic Host Cells Comprising A Library Of At Least 103 Different Rna Molecules Each Having A Dicistronic Coding Region; For Screening A Larger Portion Of The Immunological Repertoire For Receptors Having A Preselected Activity
  • Methods For Screening For Apoptosis Modulators Using Nade, P75ntr-Associated Cell Death Executor
  • Engineered Zinc Finger Proteins For Regulation Of Gene Expression
  • Nucleic Acid Library Generation Methods And Compositions
  • Trichoderma Β-Glucosidase
  • Yeast Cells Expressing Modified G Proteins And Methods Of Use Therefor
  • Leafhopper Ecdysone Receptor Nucleic Acids, Polypeptides, And Uses Thereof
  • Cell-Free Synthesis And Isolation Of Novel Genes And Polypeptides
  • System For Pulling Out Regulatory Elements Using Yeast
  • New Method Of Detecting And Analysing Protein Interactions In Vivo
  • Totally Synthetic Affinity Reagents
  • Compositions And Methods For Molecular Labeling
  • Egvii Endoglucanase And Nucleic Acids Encoding The Same
  • Vitro Evolution In Microfluidic Systems
  • System For Detection Of A Functional Interaction Between A Compound And A Cellular Signal Transduction Component
  • Generation Of Acyl Amino Acids
  • Two-Hybrid Based Screen To Identify Disruptive Residues At Multiple Protein Interfaces
  • Non-Human Organism Comprising A Gene Expression Modulation System Encoding A Chimeric Retinoid X Receptor
  • Secreted Proteins
  • Hybrid Nucleic Acids; Cancer Diagnosis
  • Targeting Pax2 For The Induction Of Defb1-Mediated Tumor Immunity And Cancer Therapy
  • Cell Based Signal Generation
  • Calibration Binding; Insertion Of A Dna Sequence
  • Methods And Compounds For Modulating Male Fertility
  • Expression Vectors For Use As Tools For Controlling Gene Expression In Genetic Engineering
  • Compositions For Protein Fragment Complementation Assays To Detect Biomolecular Interactions
  • Engineered Cleavage Half-Domains
  • Dimerization, Oligomerization Of Proteins; Activation Of Cellmembrane Receptors
  • Nucleic Acid Sequence Which Codes Mammalian Emzymatic Protein; For Treatment Of Hypercholesterolemia, Myocardium Necrosis, Hyperlipidemia, Dietetics, Stroke, Nutrient Defects And Diabetes
  • Vector Operably Linked To A Transcriptional Regulatory Sequence
  • Human Β-Adrenergic Receptor Kinase Polypeptide And Methods
  • Heterologous Polypeptide Production In The Absence Of Nonsense-Mediated Mrna Decay Functions
  • Fast And Exhaustive Method For Selecting A Prey Polypeptide Interacting With A Bait Polypeptide Of Interest: Application To The Construction Of Maps Of Interactors Polypeptides
  • Method And Kit For Detecting Membrane Protein-Protein Interactions
  • Methods For Forming Mixed Droplets
  • Method For Detecting And Analyzing Protein Interactions In-Vivo
  • Epitopes Related To Coeliac Disease
  • Polypeptdes Capable Of Forming Homo-Oligomers With Modular Hydrogen Bond Network-Mediated Specificity And Their Design
  • Treatment Of Metastatic Tumors
  • Nucleic Acid Adaptors And Uses Thereof
  • Isolation Of Proteins Involved In Posttranscriptional Gene Silencing And Methods Of Use
  • Targeting Pax2 For The Treatment Of Breast Cancer
  • Methods To Identify Usp1 Deubiquitinating Enzyme Complex Inhibitors
  • Eukaryotic Cell Which Codes Mutant Membrane Protein; For Use In Drug Screening
  • Dimerization Or Oligomerization Of Intracellular Proteins To Activate Genetic Transcription; Signal Transduction And Activators Of Transcription
  • Epitopes Related To Coeliac Disease
  • Methods And Compositions For Transferring Dna Sequence Information Among Vectors
  • Calcineurin Interacting Protein Compositions And Methods
  • Rationally Designed, Synthetic Antibody Libraries And Uses Therefor
  • Cloning System And Method For The Identification Of Interaction Partners In Protein Complexes
  • Adjustable Sensitivity, Genetic Molecular Interaction Systems, Including Protein-Protein Interaction Systems For Detection And Analysis
  • Cell Having Amplified Signal Transduction Pathway Responses And Uses Therefor
  • Digital Analyte Analysis
  • Chlorotoxin Polypeptides And Conjugates And Uses Thereof
  • Cellular Receptor For Hiv-1 Vpr Essential For G2/M Phase Transition Of The Cell Cycle
  • Glucanases/Glucosidase/Hydrolases; Conversion Of Cellulose To Sugars And/Or Ethanol; Laundering Detergents
  • Human Nucleotide Sequences Coding Binding Protein For Use In The Treatment Of Glandular Disorders
  • Detection Of Preferential Particle In Sample; Obtain Sample, Incubate With Probe, Detect Signal, Presence Of Signal Indicates Preferential Particle
  • Detection Of Protein To Protein Interactions
  • Detecting Preferential Polypeptides Or Nucleotide Sequences In Sample; Obtain Sample Of Biopolymers, Determine Sequences, Compare To Libraries, Evaluate Biopolymer As Potential Drug Target
  • Manipulation Of Fluids And Reactions In Microfluidic Systems
  • Analyte Detection Assay And Methods Of Use
  • Totally Synthetic Affinity Reagents
  • Use In Detecting Gene Mutations And/Or Polymorphisms; Diagnosing Cancer Susceptibility; Identifying, Classifying Mismatch-Repair-Defective Tumors; Hmlh1 And Hpms1 Genes And Gene Products; Polyclonal, Monoclonal Antibodies
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • Zinc Finger Proteins For Dna Binding And Gene Regulation In Plants
  • Manipulation Of Microfluidic Droplets
  • Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
  • System To Detect Protein-Rna Interactions
  • Leafhopper Ecdysone Receptor Nucleic Acids, Polypeptides, And Uses Thereof
  • Antibody To Secreted Polypeptide
  • Manipulation Of Microfluidic Droplets
  • Cell Based Signal Generation
  • Contacting Test Agent With Complex Comprising Translation Factor Sequestered By Cell Component Wherein Said Complex Responds To Hormone Treatment By Releasing Translation Factor From Sequestration; Detecting If Test Agent Causes Release
  • Cellulases; Beta-Glucosidases; Hydrolases; Conversion Of Cellulose To Sugars And/Or Ethanol; Laundry Detergents
  • Method Of Synthesis And Testing Of Combinatorial Libraries Using Microcapsules
  • Drosophila G Protein Coupled Receptors, Nucleic Acids, And Methods Related To The Same
  • Labelled Silica-Based Nanomaterial With Enhanced Properties And Uses Thereof
  • Catalytically Active Recombinant Memapsin And Methods Of Use Thereof
  • Cell Cycle Regulatory Protein P16 Gene
  • Inhibitor Of Cell Cycle Progression; Cell Growth Inhibitors; Specifically Binds A Cyclin Dependent Kinase (Cdk);
  • Aegyptin And Uses Thereof
  • Substitution Mutant Receptors And Their Use In A Nuclear Receptor-Based Inducible Gene Expression System
  • Anti-Bacterial Vaccine Compositions
  • Bgl4 Beta-Glucosidase And Nucleic Acids Encoding The Same
  • Polynucleotide Derived From Fungal Source Which Comprises A Nucleotide Sequence Encoding An Enzyme Having Beta-Glucosidase Activity
  • Quantitative Determining In Tissue Samples Cellular Expression Of Prostate Stem Cell Antigen Protein Using Monoclonal Antibodies; Increases Cells Indicate The Presence Of Cancer
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/340245a0

    DOI

    http://dx.doi.org/10.1038/340245a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028860985

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2547163


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Restriction Enzymes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Fungal", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA-Binding Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fungal Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plasmids", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saccharomyces cerevisiae", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Saccharomyces cerevisiae Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription Factors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transformation, Genetic", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA", 
              "id": "http://www.grid.ac/institutes/grid.36425.36", 
              "name": [
                "Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Fields", 
            "givenName": "Stanley", 
            "id": "sg:person.014624417057.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014624417057.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA", 
              "id": "http://www.grid.ac/institutes/grid.36425.36", 
              "name": [
                "Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Song", 
            "givenName": "Ok-kyu", 
            "id": "sg:person.01331165731.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331165731.27"
            ], 
            "type": "Person"
          }
        ], 
        "datePublished": "1989-07", 
        "datePublishedReg": "1989-07-01", 
        "description": "PROTEIN-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We have generated a novel genetic system to study these interactions by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae. This protein is a transcriptional activator required for the expression of genes encoding enzymes of galactose utilization1. It consists of two separable and functionally essential domains: an N-terminal domain which binds to specific DNA sequences (UASG); and a C-terminal domain containing acidic regions, which is necessary to activate transcription2,3. We have generated a system of two hybrid proteins containing parts of GAL4: the GAL4 DNA-binding domain fused to a protein 'X' and a GAL4 activating region fused to a protein 'Y'. If X and Y can form a protein-protein complex and reconstitute proximity of the GAL4 domains, tran-scription of a gene regulated by UASG occurs. We have tested this system using two yeast proteins that are known to interact\u2014SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.", 
        "genre": "article", 
        "id": "sg:pub.10.1038/340245a0", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0028-0836", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6230", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "340"
          }
        ], 
        "keywords": [
          "novel genetic system", 
          "protein-protein interactions", 
          "genetic system", 
          "GAL4 DNA-binding domain", 
          "DNA-binding domain", 
          "C-terminal domain", 
          "protein-protein complexes", 
          "N-terminal domain", 
          "specific DNA sequences", 
          "expression of genes", 
          "higher transcriptional activity", 
          "GAL4 protein", 
          "Gal4 domain", 
          "transcriptional activator", 
          "yeast proteins", 
          "yeast Saccharomyces", 
          "hybrid protein", 
          "DNA sequences", 
          "transcriptional activity", 
          "essential domains", 
          "biochemical techniques", 
          "acidic region", 
          "protein", 
          "GAL4", 
          "genes", 
          "Snf1", 
          "domain", 
          "UASG", 
          "Saccharomyces", 
          "Snf4", 
          "activator", 
          "enzyme", 
          "interaction", 
          "sequence", 
          "expression", 
          "cells", 
          "hybrids", 
          "region", 
          "complexes", 
          "selection", 
          "activity", 
          "proximity", 
          "chromatography", 
          "system", 
          "part", 
          "general method", 
          "use", 
          "advantages", 
          "properties", 
          "method", 
          "technique"
        ], 
        "name": "A novel genetic system to detect protein\u2013protein interactions", 
        "pagination": "245-246", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1028860985"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/340245a0"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2547163"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/340245a0", 
          "https://app.dimensions.ai/details/publication/pub.1028860985"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-08-04T16:50", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_186.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1038/340245a0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/340245a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/340245a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/340245a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/340245a0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    175 TRIPLES      20 PREDICATES      91 URIs      82 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/340245a0 schema:about N2d2d8c096f5b4131851b6711c99055a9
    2 N4d08a6c979324c49a0f0a00b2c42659f
    3 N4ec275bcd0824f9aa8568e5fcae1d8fa
    4 N522ea3ed041b47768063a214154c3c89
    5 N578bc994984747dc8f880b9ff1e8972a
    6 N766e604c78d7484e847edfcf7b9b2568
    7 Nb9d860d455b7473cb1562c71277a9c4f
    8 Nc16f407956db480c98da80a331d42549
    9 Ndda4aaeff05c4faeb2d295783a3dc388
    10 Ne1991721cd00401a8b9a6f4e7dc011fe
    11 Ne62e6e4b655e4320a6ee7ec0c20dfda3
    12 Nefffc2043fb64ed8874d197a8861ad85
    13 Nf0665241bc6643d0a31941ff73f24593
    14 anzsrc-for:06
    15 anzsrc-for:0601
    16 anzsrc-for:0604
    17 schema:author Ndff19e1253314bb395b5807329e012a8
    18 schema:datePublished 1989-07
    19 schema:datePublishedReg 1989-07-01
    20 schema:description PROTEIN-protein interactions between two proteins have generally been studied using biochemical techniques such as crosslinking, co-immunoprecipitation and co-fractionation by chromatography. We have generated a novel genetic system to study these interactions by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae. This protein is a transcriptional activator required for the expression of genes encoding enzymes of galactose utilization1. It consists of two separable and functionally essential domains: an N-terminal domain which binds to specific DNA sequences (UASG); and a C-terminal domain containing acidic regions, which is necessary to activate transcription2,3. We have generated a system of two hybrid proteins containing parts of GAL4: the GAL4 DNA-binding domain fused to a protein 'X' and a GAL4 activating region fused to a protein 'Y'. If X and Y can form a protein-protein complex and reconstitute proximity of the GAL4 domains, tran-scription of a gene regulated by UASG occurs. We have tested this system using two yeast proteins that are known to interact—SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell. This system may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.
    21 schema:genre article
    22 schema:isAccessibleForFree false
    23 schema:isPartOf N6ced1a0114a44bd8816c10d8b0a5e04c
    24 N96274fcef16e49019d4154235369cd37
    25 sg:journal.1018957
    26 schema:keywords C-terminal domain
    27 DNA sequences
    28 DNA-binding domain
    29 GAL4
    30 GAL4 DNA-binding domain
    31 GAL4 protein
    32 Gal4 domain
    33 N-terminal domain
    34 Saccharomyces
    35 Snf1
    36 Snf4
    37 UASG
    38 acidic region
    39 activator
    40 activity
    41 advantages
    42 biochemical techniques
    43 cells
    44 chromatography
    45 complexes
    46 domain
    47 enzyme
    48 essential domains
    49 expression
    50 expression of genes
    51 general method
    52 genes
    53 genetic system
    54 higher transcriptional activity
    55 hybrid protein
    56 hybrids
    57 interaction
    58 method
    59 novel genetic system
    60 part
    61 properties
    62 protein
    63 protein-protein complexes
    64 protein-protein interactions
    65 proximity
    66 region
    67 selection
    68 sequence
    69 specific DNA sequences
    70 system
    71 technique
    72 transcriptional activator
    73 transcriptional activity
    74 use
    75 yeast Saccharomyces
    76 yeast proteins
    77 schema:name A novel genetic system to detect protein–protein interactions
    78 schema:pagination 245-246
    79 schema:productId N028c07236b814619a35c93446fc5d63b
    80 N93be592fea544fed8fe5f114d0f1f373
    81 Ncce78c6de091429c99319019644fa1a9
    82 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028860985
    83 https://doi.org/10.1038/340245a0
    84 schema:sdDatePublished 2022-08-04T16:50
    85 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    86 schema:sdPublisher N4cb875ca18c3466a8591872e09b1e7f3
    87 schema:url https://doi.org/10.1038/340245a0
    88 sgo:license sg:explorer/license/
    89 sgo:sdDataset articles
    90 rdf:type schema:ScholarlyArticle
    91 N028c07236b814619a35c93446fc5d63b schema:name pubmed_id
    92 schema:value 2547163
    93 rdf:type schema:PropertyValue
    94 N25d57008076f454faf145f0c819dc983 rdf:first sg:person.01331165731.27
    95 rdf:rest rdf:nil
    96 N2d2d8c096f5b4131851b6711c99055a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Recombinant Proteins
    98 rdf:type schema:DefinedTerm
    99 N4cb875ca18c3466a8591872e09b1e7f3 schema:name Springer Nature - SN SciGraph project
    100 rdf:type schema:Organization
    101 N4d08a6c979324c49a0f0a00b2c42659f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name DNA-Binding Proteins
    103 rdf:type schema:DefinedTerm
    104 N4ec275bcd0824f9aa8568e5fcae1d8fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Recombinant Fusion Proteins
    106 rdf:type schema:DefinedTerm
    107 N522ea3ed041b47768063a214154c3c89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Plasmids
    109 rdf:type schema:DefinedTerm
    110 N578bc994984747dc8f880b9ff1e8972a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Mutation
    112 rdf:type schema:DefinedTerm
    113 N6ced1a0114a44bd8816c10d8b0a5e04c schema:volumeNumber 340
    114 rdf:type schema:PublicationVolume
    115 N766e604c78d7484e847edfcf7b9b2568 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    116 schema:name Saccharomyces cerevisiae Proteins
    117 rdf:type schema:DefinedTerm
    118 N93be592fea544fed8fe5f114d0f1f373 schema:name dimensions_id
    119 schema:value pub.1028860985
    120 rdf:type schema:PropertyValue
    121 N96274fcef16e49019d4154235369cd37 schema:issueNumber 6230
    122 rdf:type schema:PublicationIssue
    123 Nb9d860d455b7473cb1562c71277a9c4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Fungal Proteins
    125 rdf:type schema:DefinedTerm
    126 Nc16f407956db480c98da80a331d42549 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Transformation, Genetic
    128 rdf:type schema:DefinedTerm
    129 Ncce78c6de091429c99319019644fa1a9 schema:name doi
    130 schema:value 10.1038/340245a0
    131 rdf:type schema:PropertyValue
    132 Ndda4aaeff05c4faeb2d295783a3dc388 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Transcription Factors
    134 rdf:type schema:DefinedTerm
    135 Ndff19e1253314bb395b5807329e012a8 rdf:first sg:person.014624417057.73
    136 rdf:rest N25d57008076f454faf145f0c819dc983
    137 Ne1991721cd00401a8b9a6f4e7dc011fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    138 schema:name Transcription, Genetic
    139 rdf:type schema:DefinedTerm
    140 Ne62e6e4b655e4320a6ee7ec0c20dfda3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name DNA Restriction Enzymes
    142 rdf:type schema:DefinedTerm
    143 Nefffc2043fb64ed8874d197a8861ad85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name DNA, Fungal
    145 rdf:type schema:DefinedTerm
    146 Nf0665241bc6643d0a31941ff73f24593 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    147 schema:name Saccharomyces cerevisiae
    148 rdf:type schema:DefinedTerm
    149 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    150 schema:name Biological Sciences
    151 rdf:type schema:DefinedTerm
    152 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    153 schema:name Biochemistry and Cell Biology
    154 rdf:type schema:DefinedTerm
    155 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    156 schema:name Genetics
    157 rdf:type schema:DefinedTerm
    158 sg:journal.1018957 schema:issn 0028-0836
    159 1476-4687
    160 schema:name Nature
    161 schema:publisher Springer Nature
    162 rdf:type schema:Periodical
    163 sg:person.01331165731.27 schema:affiliation grid-institutes:grid.36425.36
    164 schema:familyName Song
    165 schema:givenName Ok-kyu
    166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01331165731.27
    167 rdf:type schema:Person
    168 sg:person.014624417057.73 schema:affiliation grid-institutes:grid.36425.36
    169 schema:familyName Fields
    170 schema:givenName Stanley
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014624417057.73
    172 rdf:type schema:Person
    173 grid-institutes:grid.36425.36 schema:alternateName Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA
    174 schema:name Department of Microbiology, State University of New York at Stony Brook, 11794, Stony Brook, New York, USA
    175 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...