Fertile transgenic rice plants regenerated from transformed protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-03

AUTHORS

Ko Shimamoto, Rie Terada, Takeshi Izawa, Hideya Fujimoto

ABSTRACT

THE generation of transgenic plants using gene transfer techniques is important to both the investigation of gene regulation and the genetic engineering of crops1. The Ti plasmid of Agrobacterium tumefaciens is now routinely used to transform dicotyledonous plants2, and the transfer of foreign genes to unorganized tissue3–6and plants7,8 has been accomplished using direct DNA transfer methods9–11. A protocol for the easy and reproducible production of fertile transgenic cereals, however, has not yet been described. We report here the production of fertile transgenic rice plants obtained by introducing the bacterial hph gene, encoding hygromycin B resistance12 (Hmr), into protoplasts of Oryza sativa (L.) by electroporation. The non-selectable gene encoding β-glucuronidase was also transferred with the hph gene and its expression was detected in the progeny of the stable transformant. More... »

PAGES

274-276

Journal

TITLE

Nature

ISSUE

6212

VOLUME

338

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  • Identifiers

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    DOI

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    17 schema:description THE generation of transgenic plants using gene transfer techniques is important to both the investigation of gene regulation and the genetic engineering of crops1. The Ti plasmid of Agrobacterium tumefaciens is now routinely used to transform dicotyledonous plants2, and the transfer of foreign genes to unorganized tissue3–6and plants7,8 has been accomplished using direct DNA transfer methods9–11. A protocol for the easy and reproducible production of fertile transgenic cereals, however, has not yet been described. We report here the production of fertile transgenic rice plants obtained by introducing the bacterial hph gene, encoding hygromycin B resistance12 (Hmr), into protoplasts of Oryza sativa (L.) by electroporation. The non-selectable gene encoding β-glucuronidase was also transferred with the hph gene and its expression was detected in the progeny of the stable transformant.
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    24 Crops1
    25 Oryza sativa
    26 Ti plasmid
    27 cereals
    28 electroporation
    29 engineering
    30 expression
    31 fertile transgenic rice plants
    32 foreign genes
    33 gene regulation
    34 gene transfer techniques
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