Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-01

AUTHORS

T A Haystead, A T Sim, D Carling, R C Honnor, Y Tsukitani, P Cohen, D G Hardie

ABSTRACT

Okadaic acid is a polyether derivative of 38-carbon fatty acid, and is implicated as the causative agent of diarrhetic shellfish poisoning. It is a potent tumour promoter that is not an activator of protein kinase C, but is a powerful inhibitor of protein phosphatases-1 and -2A (PP1 and PP2A) in vitro. We report here that okadaic acid rapidly stimulates protein phosphorylation in intact cells, and behaves like a specific protein phosphatase inhibitor in a variety of metabolic processes. Our results indicate that PP1 and PP2A are the dominant protein phosphatases acting on a wide range of phosphoproteins in vivo. We also find that okadaic acid mimics the effect of insulin on glucose transport in adipocytes, which suggests that this process is stimulated by a serine/threonine phosphorylation event. More... »

PAGES

78-81

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/337078a0

DOI

http://dx.doi.org/10.1038/337078a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004524872

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2562908


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetyl-CoA Carboxylase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adipose Tissue", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carcinogens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethers, Cyclic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Okadaic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoprotein Phosphatases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphorylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rats", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Dundee", 
          "id": "https://www.grid.ac/institutes/grid.8241.f", 
          "name": [
            "Biochemistry Department, The University, Dundee, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Haystead", 
        "givenName": "T A", 
        "id": "sg:person.01147776470.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147776470.43"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Sim", 
        "givenName": "A T", 
        "id": "sg:person.01233771033.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233771033.91"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Carling", 
        "givenName": "D", 
        "id": "sg:person.0751007256.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751007256.62"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Honnor", 
        "givenName": "R C", 
        "type": "Person"
      }, 
      {
        "familyName": "Tsukitani", 
        "givenName": "Y", 
        "id": "sg:person.01354377023.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354377023.31"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Cohen", 
        "givenName": "P", 
        "type": "Person"
      }, 
      {
        "familyName": "Hardie", 
        "givenName": "D G", 
        "id": "sg:person.0714441613.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714441613.38"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1989-01", 
    "datePublishedReg": "1989-01-01", 
    "description": "Okadaic acid is a polyether derivative of 38-carbon fatty acid, and is implicated as the causative agent of diarrhetic shellfish poisoning. It is a potent tumour promoter that is not an activator of protein kinase C, but is a powerful inhibitor of protein phosphatases-1 and -2A (PP1 and PP2A) in vitro. We report here that okadaic acid rapidly stimulates protein phosphorylation in intact cells, and behaves like a specific protein phosphatase inhibitor in a variety of metabolic processes. Our results indicate that PP1 and PP2A are the dominant protein phosphatases acting on a wide range of phosphoproteins in vivo. We also find that okadaic acid mimics the effect of insulin on glucose transport in adipocytes, which suggests that this process is stimulated by a serine/threonine phosphorylation event.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/337078a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6202", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "337"
      }
    ], 
    "name": "Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism", 
    "pagination": "78-81", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "158fd8df86f89be9673fb76b0a3c51f9a867059d41fb40d57a7c2c092bc0bc97"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "2562908"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/337078a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004524872"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/337078a0", 
      "https://app.dimensions.ai/details/publication/pub.1004524872"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T19:45", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000421.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nature/journal/v337/n6202/full/337078a0.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/337078a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/337078a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/337078a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/337078a0'


 

This table displays all metadata directly associated to this object as RDF triples.

151 TRIPLES      20 PREDICATES      41 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/337078a0 schema:about N07f799dae46c4b8ea731998643f30ccb
2 N0940dd3697164bf0823f472b4cbc659e
3 N0c0af686203b425a8a380fb84cda7073
4 N0d59fe871264486491cf998af62bc36c
5 N2533559831a045e9a14b5fe4b31a1476
6 N26727faa50be42988fc72dfdb5f02daf
7 N2f147a5600cf456d90c57ef95c4e0700
8 N31343e30e59f4c7797b95c8b33848a90
9 N3e13bb0047964e48b2cedf46f13609e6
10 N51f94c7a65514409b90e5274faa6db04
11 N64c183b1c3054963a55fd0ec8623ed11
12 N8cd24cf76f044826a8f927770d66a1ea
13 anzsrc-for:06
14 anzsrc-for:0601
15 schema:author N3935e895cb574595a79e10e0d235c4c3
16 schema:datePublished 1989-01
17 schema:datePublishedReg 1989-01-01
18 schema:description Okadaic acid is a polyether derivative of 38-carbon fatty acid, and is implicated as the causative agent of diarrhetic shellfish poisoning. It is a potent tumour promoter that is not an activator of protein kinase C, but is a powerful inhibitor of protein phosphatases-1 and -2A (PP1 and PP2A) in vitro. We report here that okadaic acid rapidly stimulates protein phosphorylation in intact cells, and behaves like a specific protein phosphatase inhibitor in a variety of metabolic processes. Our results indicate that PP1 and PP2A are the dominant protein phosphatases acting on a wide range of phosphoproteins in vivo. We also find that okadaic acid mimics the effect of insulin on glucose transport in adipocytes, which suggests that this process is stimulated by a serine/threonine phosphorylation event.
19 schema:genre research_article
20 schema:inLanguage en
21 schema:isAccessibleForFree false
22 schema:isPartOf N31804ffeae6f4b8c9f208f051b80e399
23 Nd93b2b9ac1b84f8d830920f6872bf356
24 sg:journal.1018957
25 schema:name Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism
26 schema:pagination 78-81
27 schema:productId N0fea1fddc3ca4fa9ae0ab5319ae75746
28 N1fcb8e0b4a5142b1a809d838858302fc
29 Nc2fb0e089c684c55b4e59379150686ec
30 Nda01604aa3e247e7829886e9759a8ee1
31 Ne9a05a4d178242038cd1aa6bfdee7085
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004524872
33 https://doi.org/10.1038/337078a0
34 schema:sdDatePublished 2019-04-10T19:45
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N9e72a8db2b534575b177124b3431ef5b
37 schema:url http://www.nature.com/nature/journal/v337/n6202/full/337078a0.html
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N07f799dae46c4b8ea731998643f30ccb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
42 schema:name Rats
43 rdf:type schema:DefinedTerm
44 N0940dd3697164bf0823f472b4cbc659e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
45 schema:name Okadaic Acid
46 rdf:type schema:DefinedTerm
47 N0c0af686203b425a8a380fb84cda7073 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Ethers, Cyclic
49 rdf:type schema:DefinedTerm
50 N0d59fe871264486491cf998af62bc36c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Liver
52 rdf:type schema:DefinedTerm
53 N0fea1fddc3ca4fa9ae0ab5319ae75746 schema:name dimensions_id
54 schema:value pub.1004524872
55 rdf:type schema:PropertyValue
56 N1fcb8e0b4a5142b1a809d838858302fc schema:name readcube_id
57 schema:value 158fd8df86f89be9673fb76b0a3c51f9a867059d41fb40d57a7c2c092bc0bc97
58 rdf:type schema:PropertyValue
59 N2533559831a045e9a14b5fe4b31a1476 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Phosphoprotein Phosphatases
61 rdf:type schema:DefinedTerm
62 N26727faa50be42988fc72dfdb5f02daf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Carcinogens
64 rdf:type schema:DefinedTerm
65 N2c11c1099cf64d0c8a9fa9bc1d61820c rdf:first sg:person.0751007256.62
66 rdf:rest Nc54f78fb39e54541a1b4500b104c543a
67 N2f147a5600cf456d90c57ef95c4e0700 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Phosphorylation
69 rdf:type schema:DefinedTerm
70 N31343e30e59f4c7797b95c8b33848a90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Animals
72 rdf:type schema:DefinedTerm
73 N31804ffeae6f4b8c9f208f051b80e399 schema:volumeNumber 337
74 rdf:type schema:PublicationVolume
75 N344cc4e6e0704242ae4055e11bfb0783 rdf:first Ncee6e77dcd7e45db815533e2a0587b73
76 rdf:rest Nfccc8fe9fe51491aa4db52ebb4cdea2c
77 N3935e895cb574595a79e10e0d235c4c3 rdf:first sg:person.01147776470.43
78 rdf:rest N94dfb63f029e4e0a87f9da6ad621bc8f
79 N3e13bb0047964e48b2cedf46f13609e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Proteins
81 rdf:type schema:DefinedTerm
82 N51f94c7a65514409b90e5274faa6db04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Acetyl-CoA Carboxylase
84 rdf:type schema:DefinedTerm
85 N64c183b1c3054963a55fd0ec8623ed11 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Adipose Tissue
87 rdf:type schema:DefinedTerm
88 N6e99061b1bda4e29829a776cacd040c8 rdf:first sg:person.01354377023.31
89 rdf:rest N344cc4e6e0704242ae4055e11bfb0783
90 N8cd24cf76f044826a8f927770d66a1ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Glucose
92 rdf:type schema:DefinedTerm
93 N94dfb63f029e4e0a87f9da6ad621bc8f rdf:first sg:person.01233771033.91
94 rdf:rest N2c11c1099cf64d0c8a9fa9bc1d61820c
95 N9e72a8db2b534575b177124b3431ef5b schema:name Springer Nature - SN SciGraph project
96 rdf:type schema:Organization
97 Nc2fb0e089c684c55b4e59379150686ec schema:name doi
98 schema:value 10.1038/337078a0
99 rdf:type schema:PropertyValue
100 Nc54f78fb39e54541a1b4500b104c543a rdf:first Neaa75898af30456b85966da24a90290a
101 rdf:rest N6e99061b1bda4e29829a776cacd040c8
102 Ncee6e77dcd7e45db815533e2a0587b73 schema:familyName Cohen
103 schema:givenName P
104 rdf:type schema:Person
105 Nd93b2b9ac1b84f8d830920f6872bf356 schema:issueNumber 6202
106 rdf:type schema:PublicationIssue
107 Nda01604aa3e247e7829886e9759a8ee1 schema:name pubmed_id
108 schema:value 2562908
109 rdf:type schema:PropertyValue
110 Ne9a05a4d178242038cd1aa6bfdee7085 schema:name nlm_unique_id
111 schema:value 0410462
112 rdf:type schema:PropertyValue
113 Neaa75898af30456b85966da24a90290a schema:familyName Honnor
114 schema:givenName R C
115 rdf:type schema:Person
116 Nfccc8fe9fe51491aa4db52ebb4cdea2c rdf:first sg:person.0714441613.38
117 rdf:rest rdf:nil
118 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biological Sciences
120 rdf:type schema:DefinedTerm
121 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
122 schema:name Biochemistry and Cell Biology
123 rdf:type schema:DefinedTerm
124 sg:journal.1018957 schema:issn 0090-0028
125 1476-4687
126 schema:name Nature
127 rdf:type schema:Periodical
128 sg:person.01147776470.43 schema:affiliation https://www.grid.ac/institutes/grid.8241.f
129 schema:familyName Haystead
130 schema:givenName T A
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147776470.43
132 rdf:type schema:Person
133 sg:person.01233771033.91 schema:familyName Sim
134 schema:givenName A T
135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01233771033.91
136 rdf:type schema:Person
137 sg:person.01354377023.31 schema:familyName Tsukitani
138 schema:givenName Y
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354377023.31
140 rdf:type schema:Person
141 sg:person.0714441613.38 schema:familyName Hardie
142 schema:givenName D G
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714441613.38
144 rdf:type schema:Person
145 sg:person.0751007256.62 schema:familyName Carling
146 schema:givenName D
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751007256.62
148 rdf:type schema:Person
149 https://www.grid.ac/institutes/grid.8241.f schema:alternateName University of Dundee
150 schema:name Biochemistry Department, The University, Dundee, UK.
151 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...