Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-11

AUTHORS

S L Mansour, K R Thomas, M R Capecchi

ABSTRACT

Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene. More... »

PAGES

348-352

Journal

TITLE

Nature

ISSUE

6197

VOLUME

336

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/336348a0

    DOI

    http://dx.doi.org/10.1038/336348a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024391427

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3194019


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