Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-03

AUTHORS

Richard A. Steinhardt, Janet Alderton

ABSTRACT

Cytosolic free calcium has recently been implicated in the regulation of mitosis in plant and animal cells1–8. We have previously found correlations between increases in the levels of intracellular free calcium [Ca2+]i and visible transitions of structure at nuclear envelope breakdown (NEBD) and the onset of anaphase during mitosis in sea urchin embryos and tissue culture cells7,8. To go beyond correlations it is necessary to manipulate [Ca2+]i, and in sea urchin embryos this requires the injection of calcium-chelator buffer solutions as the changes in free calcium in the cell cycle are dependent on intracellular stores7,9. We report here that blocking the increase in [Ca2+li which just precedes NEBD prevents this from taking place and halts mitosis. Subsequent injections which momentarily increase [Ca2+]i, or a natural recovery of the higher calcium levels, result in NEBD and the successful continuation of mitosis. Similarly, artificially increasing calcium by early injections results in early NEBD. We conclude that the increase in [Ca2+]i> preceding NEBD is an essential regulatory step required for entry into mitosis. More... »

PAGES

364-366

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/332364a0

DOI

http://dx.doi.org/10.1038/332364a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005395234

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3127727


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1116", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Physiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ammonia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Egtazic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fertilization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intracellular Fluid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mitosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nuclear Envelope", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sea Urchins", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Zoology, University of California, 94720, Berkeley, California, USA", 
          "id": "http://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Zoology, University of California, 94720, Berkeley, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Steinhardt", 
        "givenName": "Richard A.", 
        "id": "sg:person.0744066121.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744066121.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Zoology, University of California, 94720, Berkeley, California, USA", 
          "id": "http://www.grid.ac/institutes/grid.47840.3f", 
          "name": [
            "Department of Zoology, University of California, 94720, Berkeley, California, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alderton", 
        "givenName": "Janet", 
        "id": "sg:person.01127473275.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127473275.77"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/315147a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025448834", 
          "https://doi.org/10.1038/315147a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/316848a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052435742", 
          "https://doi.org/10.1038/316848a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1988-03", 
    "datePublishedReg": "1988-03-01", 
    "description": "Abstract Cytosolic free calcium has recently been implicated in the regulation of mitosis in plant and animal cells1\u20138. We have previously found correlations between increases in the levels of intracellular free calcium [Ca2+]i and visible transitions of structure at nuclear envelope breakdown (NEBD) and the onset of anaphase during mitosis in sea urchin embryos and tissue culture cells7,8. To go beyond correlations it is necessary to manipulate [Ca2+]i, and in sea urchin embryos this requires the injection of calcium-chelator buffer solutions as the changes in free calcium in the cell cycle are dependent on intracellular stores7,9. We report here that blocking the increase in [Ca2+li which just precedes NEBD prevents this from taking place and halts mitosis. Subsequent injections which momentarily increase [Ca2+]i, or a natural recovery of the higher calcium levels, result in NEBD and the successful continuation of mitosis. Similarly, artificially increasing calcium by early injections results in early NEBD. We conclude that the increase in [Ca2+]i> preceding NEBD is an essential regulatory step required for entry into mitosis.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/332364a0", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6162", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "332"
      }
    ], 
    "keywords": [
      "free calcium", 
      "intracellular free calcium", 
      "cytosolic free calcium", 
      "calcium", 
      "cell cycle", 
      "tissue culture", 
      "injection", 
      "onset", 
      "intracellular", 
      "Cells1", 
      "increase", 
      "regulation of mitosis", 
      "correlation", 
      "embryos", 
      "levels", 
      "nuclear envelope breakdown", 
      "regulation", 
      "mitosis", 
      "culture", 
      "changes", 
      "sea urchin embryos", 
      "envelope breakdown", 
      "breakdown", 
      "urchin embryos", 
      "cycle", 
      "onset of anaphase", 
      "buffer solution", 
      "anaphase", 
      "transition", 
      "structure", 
      "solution", 
      "plants", 
      "visible transitions", 
      "Abstract Cytosolic free calcium", 
      "animal cells1", 
      "calcium-chelator buffer solutions"
    ], 
    "name": "Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo", 
    "pagination": "364-366", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005395234"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/332364a0"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "3127727"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/332364a0", 
      "https://app.dimensions.ai/details/publication/pub.1005395234"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_200.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/332364a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/332364a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/332364a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/332364a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/332364a0'


 

This table displays all metadata directly associated to this object as RDF triples.

149 TRIPLES      22 PREDICATES      74 URIs      64 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/332364a0 schema:about N092d7914911d420fbfb2e2264d9a5b00
2 N19fe268bb9724e31afbf01942236b62e
3 N41c41e17dc86459a898e9bdcf16b74c3
4 N86cf29bf5ead44688cfd7e6e4f71870a
5 Nb8d6d277a0a44f70a0b9470605ea6ee8
6 Nc78aa151f013436091e788f7ad8fefc8
7 Nd7c1c5a7076a4b4dafd4fea968be2e7a
8 Nf4ba4fb678fe4ded9fa996488eeb1ff3
9 Nf731b005458d433c92dc286e938cc6d4
10 anzsrc-for:11
11 anzsrc-for:1116
12 schema:author N659b09dd09e6460cb3befa17559a19f0
13 schema:citation sg:pub.10.1038/315147a0
14 sg:pub.10.1038/316848a0
15 schema:datePublished 1988-03
16 schema:datePublishedReg 1988-03-01
17 schema:description Abstract Cytosolic free calcium has recently been implicated in the regulation of mitosis in plant and animal cells1–8. We have previously found correlations between increases in the levels of intracellular free calcium [Ca2+]i and visible transitions of structure at nuclear envelope breakdown (NEBD) and the onset of anaphase during mitosis in sea urchin embryos and tissue culture cells7,8. To go beyond correlations it is necessary to manipulate [Ca2+]i, and in sea urchin embryos this requires the injection of calcium-chelator buffer solutions as the changes in free calcium in the cell cycle are dependent on intracellular stores7,9. We report here that blocking the increase in [Ca2+li which just precedes NEBD prevents this from taking place and halts mitosis. Subsequent injections which momentarily increase [Ca2+]i, or a natural recovery of the higher calcium levels, result in NEBD and the successful continuation of mitosis. Similarly, artificially increasing calcium by early injections results in early NEBD. We conclude that the increase in [Ca2+]i> preceding NEBD is an essential regulatory step required for entry into mitosis.
18 schema:genre article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf N00b291e572b849049db33807e6318037
22 N63b906d7cdc742278f9b28b350e64d69
23 sg:journal.1018957
24 schema:keywords Abstract Cytosolic free calcium
25 Cells1
26 anaphase
27 animal cells1
28 breakdown
29 buffer solution
30 calcium
31 calcium-chelator buffer solutions
32 cell cycle
33 changes
34 correlation
35 culture
36 cycle
37 cytosolic free calcium
38 embryos
39 envelope breakdown
40 free calcium
41 increase
42 injection
43 intracellular
44 intracellular free calcium
45 levels
46 mitosis
47 nuclear envelope breakdown
48 onset
49 onset of anaphase
50 plants
51 regulation
52 regulation of mitosis
53 sea urchin embryos
54 solution
55 structure
56 tissue culture
57 transition
58 urchin embryos
59 visible transitions
60 schema:name Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo
61 schema:pagination 364-366
62 schema:productId N3e685d46f8574b71aef51777481ce706
63 N504cb9b9d429453cac7b26d73748d10a
64 N9febdf795c7443329b2698dab7ae69ec
65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005395234
66 https://doi.org/10.1038/332364a0
67 schema:sdDatePublished 2021-12-01T19:05
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N4808158fd9d845a0959a51fe02f7737e
70 schema:url https://doi.org/10.1038/332364a0
71 sgo:license sg:explorer/license/
72 sgo:sdDataset articles
73 rdf:type schema:ScholarlyArticle
74 N00b291e572b849049db33807e6318037 schema:issueNumber 6162
75 rdf:type schema:PublicationIssue
76 N092d7914911d420fbfb2e2264d9a5b00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Egtazic Acid
78 rdf:type schema:DefinedTerm
79 N19fe268bb9724e31afbf01942236b62e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Fertilization
81 rdf:type schema:DefinedTerm
82 N3e685d46f8574b71aef51777481ce706 schema:name pubmed_id
83 schema:value 3127727
84 rdf:type schema:PropertyValue
85 N41c41e17dc86459a898e9bdcf16b74c3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Mitosis
87 rdf:type schema:DefinedTerm
88 N4808158fd9d845a0959a51fe02f7737e schema:name Springer Nature - SN SciGraph project
89 rdf:type schema:Organization
90 N504cb9b9d429453cac7b26d73748d10a schema:name dimensions_id
91 schema:value pub.1005395234
92 rdf:type schema:PropertyValue
93 N63b906d7cdc742278f9b28b350e64d69 schema:volumeNumber 332
94 rdf:type schema:PublicationVolume
95 N659b09dd09e6460cb3befa17559a19f0 rdf:first sg:person.0744066121.13
96 rdf:rest Ndd43b1a071f54f8084d14841928b8147
97 N86cf29bf5ead44688cfd7e6e4f71870a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Sea Urchins
99 rdf:type schema:DefinedTerm
100 N9febdf795c7443329b2698dab7ae69ec schema:name doi
101 schema:value 10.1038/332364a0
102 rdf:type schema:PropertyValue
103 Nb8d6d277a0a44f70a0b9470605ea6ee8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Ammonia
105 rdf:type schema:DefinedTerm
106 Nc78aa151f013436091e788f7ad8fefc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Nuclear Envelope
108 rdf:type schema:DefinedTerm
109 Nd7c1c5a7076a4b4dafd4fea968be2e7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Intracellular Fluid
111 rdf:type schema:DefinedTerm
112 Ndd43b1a071f54f8084d14841928b8147 rdf:first sg:person.01127473275.77
113 rdf:rest rdf:nil
114 Nf4ba4fb678fe4ded9fa996488eeb1ff3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Calcium
116 rdf:type schema:DefinedTerm
117 Nf731b005458d433c92dc286e938cc6d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Animals
119 rdf:type schema:DefinedTerm
120 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
121 schema:name Medical and Health Sciences
122 rdf:type schema:DefinedTerm
123 anzsrc-for:1116 schema:inDefinedTermSet anzsrc-for:
124 schema:name Medical Physiology
125 rdf:type schema:DefinedTerm
126 sg:journal.1018957 schema:issn 0028-0836
127 1476-4687
128 schema:name Nature
129 schema:publisher Springer Nature
130 rdf:type schema:Periodical
131 sg:person.01127473275.77 schema:affiliation grid-institutes:grid.47840.3f
132 schema:familyName Alderton
133 schema:givenName Janet
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127473275.77
135 rdf:type schema:Person
136 sg:person.0744066121.13 schema:affiliation grid-institutes:grid.47840.3f
137 schema:familyName Steinhardt
138 schema:givenName Richard A.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0744066121.13
140 rdf:type schema:Person
141 sg:pub.10.1038/315147a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025448834
142 https://doi.org/10.1038/315147a0
143 rdf:type schema:CreativeWork
144 sg:pub.10.1038/316848a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052435742
145 https://doi.org/10.1038/316848a0
146 rdf:type schema:CreativeWork
147 grid-institutes:grid.47840.3f schema:alternateName Department of Zoology, University of California, 94720, Berkeley, California, USA
148 schema:name Department of Zoology, University of California, 94720, Berkeley, California, USA
149 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...