Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-12

AUTHORS

S Y Kao, A F Calman, P A Luciw, B M Peterlin

ABSTRACT

Human immunodeficiency virus-1 (HIV-1) gene expression is controlled by cellular transcription factors and by virally encoded trans-activation proteins of the HIV-1 tat and art/trs genes, which are essential for viral replication. Tat trans-activates HIV-1 gene expression by interacting with the trans-acting response element (TAR) located within the HIV-1 long terminal repeat (LTR) (ref. 2). In transient expression assays, tat mediates its effects largely by increasing the steady-state levels of messenger RNA species that contain the TAR sequence at or near their 5' ends, suggesting a function for tat either in transcription or in subsequent RNA processing. The tat gene could also facilitate translation of mRNA containing the TAR sequence. To determine the mechanism of trans-activation by tat, we analysed the structure and rate of synthesis of RNA species directed by the HIV-1 LTR in transient expression assays both in the presence and absence of tat. Although the rate of HIV-1 transcription initiation was not affected by tat, transcriptional elongation beyond position +59 was seen only in the presence of tat. Thus, tat trans-activates HIV-1 transcription by relieving a specific block to transcriptional elongation within the TAR sequence. More... »

PAGES

489-493

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/330489a0

DOI

http://dx.doi.org/10.1038/330489a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026049650

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2825027


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Acetyltransferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chloramphenicol O-Acetyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Products, tat", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HIV", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nucleic Acid Hybridization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oncogene Proteins, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Biosynthesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Repetitive Sequences, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transfection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "tat Gene Products, Human Immunodeficiency Virus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of California, San Francisco", 
          "id": "https://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kao", 
        "givenName": "S Y", 
        "type": "Person"
      }, 
      {
        "familyName": "Calman", 
        "givenName": "A F", 
        "id": "sg:person.01213431055.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213431055.37"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Luciw", 
        "givenName": "P A", 
        "id": "sg:person.01204203650.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204203650.18"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Peterlin", 
        "givenName": "B M", 
        "id": "sg:person.01012551444.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012551444.26"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1987-12", 
    "datePublishedReg": "1987-12-01", 
    "description": "Human immunodeficiency virus-1 (HIV-1) gene expression is controlled by cellular transcription factors and by virally encoded trans-activation proteins of the HIV-1 tat and art/trs genes, which are essential for viral replication. Tat trans-activates HIV-1 gene expression by interacting with the trans-acting response element (TAR) located within the HIV-1 long terminal repeat (LTR) (ref. 2). In transient expression assays, tat mediates its effects largely by increasing the steady-state levels of messenger RNA species that contain the TAR sequence at or near their 5' ends, suggesting a function for tat either in transcription or in subsequent RNA processing. The tat gene could also facilitate translation of mRNA containing the TAR sequence. To determine the mechanism of trans-activation by tat, we analysed the structure and rate of synthesis of RNA species directed by the HIV-1 LTR in transient expression assays both in the presence and absence of tat. Although the rate of HIV-1 transcription initiation was not affected by tat, transcriptional elongation beyond position +59 was seen only in the presence of tat. Thus, tat trans-activates HIV-1 transcription by relieving a specific block to transcriptional elongation within the TAR sequence.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/330489a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6147", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "330"
      }
    ], 
    "name": "Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product", 
    "pagination": "489-493", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "7c19723f27a1be9be54185affeffe10a3f8e600ebee4be445ecf3a8991ea66e8"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "2825027"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/330489a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026049650"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/330489a0", 
      "https://app.dimensions.ai/details/publication/pub.1026049650"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000424.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nature/journal/v330/n6147/full/330489a0.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/330489a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/330489a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/330489a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/330489a0'


 

This table displays all metadata directly associated to this object as RDF triples.

158 TRIPLES      20 PREDICATES      47 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/330489a0 schema:about N121807169a064cf58f42d783ca2d8483
2 N133004ce0e9547259b48449c65de95ec
3 N21cfe25491a14537adb98f4362d01bb1
4 N2c5aa972d6d84257a96adaf6397e5875
5 N3730d682c7724934a8b6f5f84126a60a
6 N415f98632d964649b92a9e027ed9d0ca
7 N428ad67d54734129b0caae137ac3ba53
8 N4d752d9f7e94466eb85166e663dbf909
9 N91601057d2ce47a4bbad90f0c20e1e4a
10 N9644cb53525a42a3ae802799fc4266a9
11 N98626bb2b26043a58a35b10f5ce22457
12 Na6a844aae4904b26b23c5de943a12828
13 Naeb4675928d44a6995b8cdd8287fb983
14 Nbf09044e0139478c8755adb215ef11e9
15 Nc32b538ff6fa4b6c83dd5b35b2496cde
16 Ncfd301d7bda546fd94d975eeaeff1f40
17 Ne06d15529cf043fd9a7f251a176f7b1e
18 Nec9fb8998b6a4cf38255b9628036f215
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author N93637926f07e49a5a67073f03fba322c
22 schema:datePublished 1987-12
23 schema:datePublishedReg 1987-12-01
24 schema:description Human immunodeficiency virus-1 (HIV-1) gene expression is controlled by cellular transcription factors and by virally encoded trans-activation proteins of the HIV-1 tat and art/trs genes, which are essential for viral replication. Tat trans-activates HIV-1 gene expression by interacting with the trans-acting response element (TAR) located within the HIV-1 long terminal repeat (LTR) (ref. 2). In transient expression assays, tat mediates its effects largely by increasing the steady-state levels of messenger RNA species that contain the TAR sequence at or near their 5' ends, suggesting a function for tat either in transcription or in subsequent RNA processing. The tat gene could also facilitate translation of mRNA containing the TAR sequence. To determine the mechanism of trans-activation by tat, we analysed the structure and rate of synthesis of RNA species directed by the HIV-1 LTR in transient expression assays both in the presence and absence of tat. Although the rate of HIV-1 transcription initiation was not affected by tat, transcriptional elongation beyond position +59 was seen only in the presence of tat. Thus, tat trans-activates HIV-1 transcription by relieving a specific block to transcriptional elongation within the TAR sequence.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf Nd8a71a007a4d46c49c03fd171a905f02
29 Ne3c91305956449beae4e083eabdfb0ae
30 sg:journal.1018957
31 schema:name Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product
32 schema:pagination 489-493
33 schema:productId N3cecb0911b3b4b1e848afac2b71da840
34 N4b524f13c768461ab63fd8270b75e847
35 N4ea96503fc3e41c8af401cc7315c0806
36 Nba253fec5eef47d3b331883ef85541dc
37 Nf9901ae7b3aa4f67aaf8d9c939b30fc6
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026049650
39 https://doi.org/10.1038/330489a0
40 schema:sdDatePublished 2019-04-11T00:55
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher N67ec3c71b8bf4b938d6b99ca4490f2f2
43 schema:url http://www.nature.com/nature/journal/v330/n6147/full/330489a0.html
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N121807169a064cf58f42d783ca2d8483 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Transcription Factors
49 rdf:type schema:DefinedTerm
50 N133004ce0e9547259b48449c65de95ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Transfection
52 rdf:type schema:DefinedTerm
53 N21cfe25491a14537adb98f4362d01bb1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
54 schema:name Chloramphenicol O-Acetyltransferase
55 rdf:type schema:DefinedTerm
56 N2c5aa972d6d84257a96adaf6397e5875 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
57 schema:name tat Gene Products, Human Immunodeficiency Virus
58 rdf:type schema:DefinedTerm
59 N3730d682c7724934a8b6f5f84126a60a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
60 schema:name Protein Biosynthesis
61 rdf:type schema:DefinedTerm
62 N3cecb0911b3b4b1e848afac2b71da840 schema:name readcube_id
63 schema:value 7c19723f27a1be9be54185affeffe10a3f8e600ebee4be445ecf3a8991ea66e8
64 rdf:type schema:PropertyValue
65 N415f98632d964649b92a9e027ed9d0ca schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Genes, Viral
67 rdf:type schema:DefinedTerm
68 N428ad67d54734129b0caae137ac3ba53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Oncogene Proteins, Viral
70 rdf:type schema:DefinedTerm
71 N4b524f13c768461ab63fd8270b75e847 schema:name nlm_unique_id
72 schema:value 0410462
73 rdf:type schema:PropertyValue
74 N4d752d9f7e94466eb85166e663dbf909 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Transcription, Genetic
76 rdf:type schema:DefinedTerm
77 N4ea96503fc3e41c8af401cc7315c0806 schema:name doi
78 schema:value 10.1038/330489a0
79 rdf:type schema:PropertyValue
80 N67ec3c71b8bf4b938d6b99ca4490f2f2 schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 N77b76c6ec14143ffafe3c4fcd140269a rdf:first sg:person.01204203650.18
83 rdf:rest Nf3e209a8e86f4b96b6e7aa564059e0fa
84 N91601057d2ce47a4bbad90f0c20e1e4a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Gene Products, tat
86 rdf:type schema:DefinedTerm
87 N93637926f07e49a5a67073f03fba322c rdf:first Nffdc780a8d084e60b605af61170e909b
88 rdf:rest Nbe52e84339aa4e92aa3ac0ce3e9745a0
89 N9644cb53525a42a3ae802799fc4266a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Acetyltransferases
91 rdf:type schema:DefinedTerm
92 N98626bb2b26043a58a35b10f5ce22457 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Plasmids
94 rdf:type schema:DefinedTerm
95 Na6a844aae4904b26b23c5de943a12828 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name RNA, Viral
97 rdf:type schema:DefinedTerm
98 Naeb4675928d44a6995b8cdd8287fb983 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Cell Line
100 rdf:type schema:DefinedTerm
101 Nba253fec5eef47d3b331883ef85541dc schema:name dimensions_id
102 schema:value pub.1026049650
103 rdf:type schema:PropertyValue
104 Nbe52e84339aa4e92aa3ac0ce3e9745a0 rdf:first sg:person.01213431055.37
105 rdf:rest N77b76c6ec14143ffafe3c4fcd140269a
106 Nbf09044e0139478c8755adb215ef11e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name RNA, Messenger
108 rdf:type schema:DefinedTerm
109 Nc32b538ff6fa4b6c83dd5b35b2496cde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Virus Replication
111 rdf:type schema:DefinedTerm
112 Ncfd301d7bda546fd94d975eeaeff1f40 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Repetitive Sequences, Nucleic Acid
114 rdf:type schema:DefinedTerm
115 Nd8a71a007a4d46c49c03fd171a905f02 schema:issueNumber 6147
116 rdf:type schema:PublicationIssue
117 Ne06d15529cf043fd9a7f251a176f7b1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Nucleic Acid Hybridization
119 rdf:type schema:DefinedTerm
120 Ne3c91305956449beae4e083eabdfb0ae schema:volumeNumber 330
121 rdf:type schema:PublicationVolume
122 Nec9fb8998b6a4cf38255b9628036f215 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name HIV
124 rdf:type schema:DefinedTerm
125 Nf3e209a8e86f4b96b6e7aa564059e0fa rdf:first sg:person.01012551444.26
126 rdf:rest rdf:nil
127 Nf9901ae7b3aa4f67aaf8d9c939b30fc6 schema:name pubmed_id
128 schema:value 2825027
129 rdf:type schema:PropertyValue
130 Nffdc780a8d084e60b605af61170e909b schema:affiliation https://www.grid.ac/institutes/grid.266102.1
131 schema:familyName Kao
132 schema:givenName S Y
133 rdf:type schema:Person
134 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
135 schema:name Biological Sciences
136 rdf:type schema:DefinedTerm
137 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
138 schema:name Genetics
139 rdf:type schema:DefinedTerm
140 sg:journal.1018957 schema:issn 0090-0028
141 1476-4687
142 schema:name Nature
143 rdf:type schema:Periodical
144 sg:person.01012551444.26 schema:familyName Peterlin
145 schema:givenName B M
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01012551444.26
147 rdf:type schema:Person
148 sg:person.01204203650.18 schema:familyName Luciw
149 schema:givenName P A
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204203650.18
151 rdf:type schema:Person
152 sg:person.01213431055.37 schema:familyName Calman
153 schema:givenName A F
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213431055.37
155 rdf:type schema:Person
156 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
157 schema:name Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143.
158 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...