p53 and DNA polymerase α compete for binding to SV40 T antigen View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-10

AUTHORS

J V Gannon, D P Lane

ABSTRACT

The large T antigen (T) of simian virus 40 is a multifunctional protein required for both viral DNA replication and cellular transformation. T antigen forms specific protein complexes with the host protein p53 in both virus-infected and transformed cells. p53 has recently been shown to be an oncogene, but its normal function is not clear. We previously established a radioimmunoassay to study the newly described complex between T antigen and DNA polymerase alpha, and have noted a similarity between the antigenic changes induced in T by the binding of both p53 and polymerase. We now extend this analysis to a larger collection of anti-T antibodies and formally establish that p53 and DNA polymerase alpha can compete for binding to the SV40 T antigen. At a critical concentration of the three components it is possible to detect a trimeric complex of T, p53 and DNA polymerase alpha. Our observations have important implications for the control by these nuclear oncogenes of viral and cellular DNA synthesis and viral host range in both normal and transformed cells. We present a model for the action of p53 in growth control. More... »

PAGES

456-458

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/329456a0

DOI

http://dx.doi.org/10.1038/329456a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049701261

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3309672


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Polyomavirus Transforming", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Binding, Competitive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Transformation, Neoplastic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Polymerase II", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HeLa Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macromolecular Substances", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neoplasm Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protein Binding", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Radioimmunoassay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Suppressor Protein p53", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Cancer Research UK", 
          "id": "https://www.grid.ac/institutes/grid.11485.39", 
          "name": [
            "Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, UK."
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gannon", 
        "givenName": "J V", 
        "id": "sg:person.0776714673.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776714673.06"
        ], 
        "type": "Person"
      }, 
      {
        "familyName": "Lane", 
        "givenName": "D P", 
        "id": "sg:person.01034432262.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034432262.26"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1987-10", 
    "datePublishedReg": "1987-10-01", 
    "description": "The large T antigen (T) of simian virus 40 is a multifunctional protein required for both viral DNA replication and cellular transformation. T antigen forms specific protein complexes with the host protein p53 in both virus-infected and transformed cells. p53 has recently been shown to be an oncogene, but its normal function is not clear. We previously established a radioimmunoassay to study the newly described complex between T antigen and DNA polymerase alpha, and have noted a similarity between the antigenic changes induced in T by the binding of both p53 and polymerase. We now extend this analysis to a larger collection of anti-T antibodies and formally establish that p53 and DNA polymerase alpha can compete for binding to the SV40 T antigen. At a critical concentration of the three components it is possible to detect a trimeric complex of T, p53 and DNA polymerase alpha. Our observations have important implications for the control by these nuclear oncogenes of viral and cellular DNA synthesis and viral host range in both normal and transformed cells. We present a model for the action of p53 in growth control.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/329456a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6138", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "329"
      }
    ], 
    "name": "p53 and DNA polymerase \u03b1 compete for binding to SV40 T antigen", 
    "pagination": "456-458", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ecc2d2e4e578f77a68a5809c3adf377b60e703a29a6b5f35972d58f9172629e9"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "3309672"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/329456a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1049701261"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/329456a0", 
      "https://app.dimensions.ai/details/publication/pub.1049701261"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:35", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8684_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/nature/journal/v329/n6138/full/329456a0.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/329456a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/329456a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/329456a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/329456a0'


 

This table displays all metadata directly associated to this object as RDF triples.

131 TRIPLES      20 PREDICATES      43 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/329456a0 schema:about N2171ebe7bc92460bbe79791b1bf71f2b
2 N2bf7379d24d14e2798bacaf69a955c49
3 N43f13a99c5ed4ae6ac4532e9e8c514c4
4 N5ad727c5f1d847749b2658dfde9f54e5
5 N6466489f5db64c95833372f0965d498a
6 N974e740a36bd4698b5bca36a65572b3b
7 N9a4155d2d5f64fa8abe72bc158b923fe
8 N9b508b2a3e844286a3224d694a14fe1e
9 Na3e117d139a34931ac1b107dfe6e60d7
10 Nb3f51c750f5e4e538a2e9e39c4dc5139
11 Nc1a636fbb5b64f608ce9b43d78f77d93
12 Ndeabb8dfa98e41cbb5edee647475b9ec
13 Ne358668e9e6a4d69b07598a0a09db84f
14 Nf672c8399d044b7db6ff4ea931dc82b6
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N75e29cd8aeb844d981bb7bfb4b163665
18 schema:datePublished 1987-10
19 schema:datePublishedReg 1987-10-01
20 schema:description The large T antigen (T) of simian virus 40 is a multifunctional protein required for both viral DNA replication and cellular transformation. T antigen forms specific protein complexes with the host protein p53 in both virus-infected and transformed cells. p53 has recently been shown to be an oncogene, but its normal function is not clear. We previously established a radioimmunoassay to study the newly described complex between T antigen and DNA polymerase alpha, and have noted a similarity between the antigenic changes induced in T by the binding of both p53 and polymerase. We now extend this analysis to a larger collection of anti-T antibodies and formally establish that p53 and DNA polymerase alpha can compete for binding to the SV40 T antigen. At a critical concentration of the three components it is possible to detect a trimeric complex of T, p53 and DNA polymerase alpha. Our observations have important implications for the control by these nuclear oncogenes of viral and cellular DNA synthesis and viral host range in both normal and transformed cells. We present a model for the action of p53 in growth control.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N36701d64783141e39362e7e0f96ecc36
25 N72d3dd65b2b843259be336cc33238d27
26 sg:journal.1018957
27 schema:name p53 and DNA polymerase α compete for binding to SV40 T antigen
28 schema:pagination 456-458
29 schema:productId N20386eb318a14be7917c2a5c3a7586f4
30 N42368f32380a4f739d3ffa7224d772fc
31 N87457cfd549b41ff999f6f4bfa875f21
32 Na0f67e6f983d4f65bcd205e84a2fee12
33 Nfbe565d4e734492d86e6ab595be0bfc5
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049701261
35 https://doi.org/10.1038/329456a0
36 schema:sdDatePublished 2019-04-10T20:35
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N5310aa4474a942cbbc4da8575ee4e06f
39 schema:url http://www.nature.com/nature/journal/v329/n6138/full/329456a0.html
40 sgo:license sg:explorer/license/
41 sgo:sdDataset articles
42 rdf:type schema:ScholarlyArticle
43 N20386eb318a14be7917c2a5c3a7586f4 schema:name pubmed_id
44 schema:value 3309672
45 rdf:type schema:PropertyValue
46 N2171ebe7bc92460bbe79791b1bf71f2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
47 schema:name Macromolecular Substances
48 rdf:type schema:DefinedTerm
49 N2bf7379d24d14e2798bacaf69a955c49 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name DNA Polymerase II
51 rdf:type schema:DefinedTerm
52 N36701d64783141e39362e7e0f96ecc36 schema:volumeNumber 329
53 rdf:type schema:PublicationVolume
54 N42368f32380a4f739d3ffa7224d772fc schema:name doi
55 schema:value 10.1038/329456a0
56 rdf:type schema:PropertyValue
57 N43f13a99c5ed4ae6ac4532e9e8c514c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Tumor Suppressor Protein p53
59 rdf:type schema:DefinedTerm
60 N5310aa4474a942cbbc4da8575ee4e06f schema:name Springer Nature - SN SciGraph project
61 rdf:type schema:Organization
62 N5ad727c5f1d847749b2658dfde9f54e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
63 schema:name Protein Binding
64 rdf:type schema:DefinedTerm
65 N6466489f5db64c95833372f0965d498a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Phosphoproteins
67 rdf:type schema:DefinedTerm
68 N72d3dd65b2b843259be336cc33238d27 schema:issueNumber 6138
69 rdf:type schema:PublicationIssue
70 N75e29cd8aeb844d981bb7bfb4b163665 rdf:first sg:person.0776714673.06
71 rdf:rest Nb5c31a9527ce447394dbb624fdfc36ed
72 N87457cfd549b41ff999f6f4bfa875f21 schema:name readcube_id
73 schema:value ecc2d2e4e578f77a68a5809c3adf377b60e703a29a6b5f35972d58f9172629e9
74 rdf:type schema:PropertyValue
75 N974e740a36bd4698b5bca36a65572b3b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name HeLa Cells
77 rdf:type schema:DefinedTerm
78 N9a4155d2d5f64fa8abe72bc158b923fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Antigens, Polyomavirus Transforming
80 rdf:type schema:DefinedTerm
81 N9b508b2a3e844286a3224d694a14fe1e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
82 schema:name Cell Transformation, Neoplastic
83 rdf:type schema:DefinedTerm
84 Na0f67e6f983d4f65bcd205e84a2fee12 schema:name dimensions_id
85 schema:value pub.1049701261
86 rdf:type schema:PropertyValue
87 Na3e117d139a34931ac1b107dfe6e60d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Neoplasm Proteins
89 rdf:type schema:DefinedTerm
90 Nb3f51c750f5e4e538a2e9e39c4dc5139 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Cell Line
92 rdf:type schema:DefinedTerm
93 Nb5c31a9527ce447394dbb624fdfc36ed rdf:first sg:person.01034432262.26
94 rdf:rest rdf:nil
95 Nc1a636fbb5b64f608ce9b43d78f77d93 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Animals
97 rdf:type schema:DefinedTerm
98 Ndeabb8dfa98e41cbb5edee647475b9ec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Binding, Competitive
100 rdf:type schema:DefinedTerm
101 Ne358668e9e6a4d69b07598a0a09db84f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Mice
103 rdf:type schema:DefinedTerm
104 Nf672c8399d044b7db6ff4ea931dc82b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Radioimmunoassay
106 rdf:type schema:DefinedTerm
107 Nfbe565d4e734492d86e6ab595be0bfc5 schema:name nlm_unique_id
108 schema:value 0410462
109 rdf:type schema:PropertyValue
110 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
111 schema:name Biological Sciences
112 rdf:type schema:DefinedTerm
113 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
114 schema:name Biochemistry and Cell Biology
115 rdf:type schema:DefinedTerm
116 sg:journal.1018957 schema:issn 0090-0028
117 1476-4687
118 schema:name Nature
119 rdf:type schema:Periodical
120 sg:person.01034432262.26 schema:familyName Lane
121 schema:givenName D P
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01034432262.26
123 rdf:type schema:Person
124 sg:person.0776714673.06 schema:affiliation https://www.grid.ac/institutes/grid.11485.39
125 schema:familyName Gannon
126 schema:givenName J V
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776714673.06
128 rdf:type schema:Person
129 https://www.grid.ac/institutes/grid.11485.39 schema:alternateName Cancer Research UK
130 schema:name Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, UK.
131 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...