Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-07

AUTHORS

Marc Boutry, Ferenc Nagy, Carsten Poulsen, Kazuko Aoyagi, Nam-Hai Chua

ABSTRACT

Most mitochondrial proteins are encoded by nuclear genes and are synthesized as precursors containing a presequence at the N terminus. In yeast and in mammalian cells, the function of the presequence in mitochondrial targeting has been revealed by chimaeric gene studies. Fusion of a mitochondrial presequence to a foreign protein coding sequence enables the protein to be imported into mitochondria in vitro as well as in vivo. Whether plant mitochondrial presequences function in the same way has been unknown. We have previously isolated and characterized a nuclear gene (atp2-1) from Nicotiana plumbaginifolia that encodes the beta-subunit of the mitochondrial ATP synthase. We have constructed a chimaeric gene comprising a putative atp2-1 presequence fused to the bacterial chloramphenicol acetyltransferase (CAT) coding sequence and introduced it into the tobacco genome. We report here that a segment of 90 amino acids of the N terminus of the beta-subunit precursor is sufficient for the specific targeting of the CAT protein to mitochondria in transgenic plants. Our results demonstrate a high specificity for organelle targeting in plant cells. More... »

PAGES

340-342

Journal

TITLE

Nature

ISSUE

6128

VOLUME

328

Related Patents

  • Transgenic Plants Including A Transgene Consisting Of A Hybrid Nucleic Acid Sequence, Comprising At Least One Unedited Mitochondrial Gene Fragment From Higher Plants And Process For Producing Them
  • Tailored Oils
  • Methods Of Producing Triacylglyceride Compositions Comprising Tailored Oils
  • Recombinant Microalgae Including Keto-Acyl Acp Synthase
  • Production Of Hydroxylated Fatty Acids In Prototheca Moriformis
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Methods For Producing A Triglyceride Composition From Algae
  • Tailored Oils
  • Hydroxylated Triacylglycerides
  • Recombinant Microalgae Cells Producing Novel Oils
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Nucleic Acids Useful In The Manufacture Of Oil
  • Recombinant Microalgae Including Sucrose Invertase And Thioesterase
  • Production Of Oil In Microorganisms
  • Microorganisms Expressing Ketoacyl-Coa Synthase And Uses Thereof
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Recombinant Microalgae Cells Producing Novel Oils
  • Processes For Producing Polyhydroxybutyrate And Related Polyhydroxyalkanoates In The Plastids Of Higher Plants
  • Renewable Fuels Produced From Oleaginous Microorganisms
  • Ketoacyl Acp Synthase Genes And Uses Thereof
  • Transgenic Plants Including A Transgene Consisting Of A Hybrid Nucleic Acid Sequence, Comprising At Least One Unedited Mitochondrial Gene Fragment From Higher Plants And Process For Producing Them
  • Control Of Gene Expression In Eukaryotes
  • Transgenic Plants Producing Polyhydroxyalkanoates
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Renewable Diesel And Jet Fuel From Microbial Sources
  • Tailored Oils
  • Tailored Oils
  • Tailored Oils
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Tailored Oils
  • Chlorella Species Containing Exogenous Genes
  • Tailored Oils Produced From Recombinant Oleaginous Microorganisms
  • Tailored Oils
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/328340a0

    DOI

    http://dx.doi.org/10.1038/328340a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051691717

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3474528


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acetyltransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chloramphenicol O-Acetyltransferase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chloroplasts", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Malate Dehydrogenase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mitochondria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants, Toxic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tobacco", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "familyName": "Boutry", 
            "givenName": "Marc", 
            "id": "sg:person.01156422660.48", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156422660.48"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Nagy", 
            "givenName": "Ferenc", 
            "id": "sg:person.012421646303.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012421646303.49"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Poulsen", 
            "givenName": "Carsten", 
            "id": "sg:person.011566154641.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011566154641.05"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Aoyagi", 
            "givenName": "Kazuko", 
            "id": "sg:person.01143656367.83", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143656367.83"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Chua", 
            "givenName": "Nam-Hai", 
            "id": "sg:person.01255377003.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255377003.26"
            ], 
            "type": "Person"
          }
        ], 
        "datePublished": "1987-07", 
        "datePublishedReg": "1987-07-01", 
        "description": "Most mitochondrial proteins are encoded by nuclear genes and are synthesized as precursors containing a presequence at the N terminus. In yeast and in mammalian cells, the function of the presequence in mitochondrial targeting has been revealed by chimaeric gene studies. Fusion of a mitochondrial presequence to a foreign protein coding sequence enables the protein to be imported into mitochondria in vitro as well as in vivo. Whether plant mitochondrial presequences function in the same way has been unknown. We have previously isolated and characterized a nuclear gene (atp2-1) from Nicotiana plumbaginifolia that encodes the beta-subunit of the mitochondrial ATP synthase. We have constructed a chimaeric gene comprising a putative atp2-1 presequence fused to the bacterial chloramphenicol acetyltransferase (CAT) coding sequence and introduced it into the tobacco genome. We report here that a segment of 90 amino acids of the N terminus of the beta-subunit precursor is sufficient for the specific targeting of the CAT protein to mitochondria in transgenic plants. Our results demonstrate a high specificity for organelle targeting in plant cells.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/328340a0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6128", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "328"
          }
        ], 
        "name": "Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants", 
        "pagination": "340-342", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "f63ba98749dd218869a84891396950c6ef262d928540c94777e92b86a9892595"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "3474528"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0410462"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/328340a0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1051691717"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/328340a0", 
          "https://app.dimensions.ai/details/publication/pub.1051691717"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T18:08", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000426.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/328340a0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/328340a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/328340a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/328340a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/328340a0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    145 TRIPLES      20 PREDICATES      43 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/328340a0 schema:about N0c1e43a241ba4a4c963b4ef9d6389069
    2 N2c269912dd1f40719b4ee53c11f59012
    3 N30eab6d02de24684a832fada10e29cd7
    4 N369f85c2383f4088bb23bc844ff6646c
    5 N3f2a89a24d2145b1a9d8d933a8881745
    6 N51588927446248d08bfd27f5a5a73ff5
    7 N65dd53eb71fd4614970c089607203488
    8 N75b88a11fd564b509101610aab61456b
    9 N82b84736930747abbb32f3dedc11d108
    10 N8c1bc09cd0c34dba9a0df12fb11aacef
    11 N8c818910209644b7ab5e95bf3d7454c0
    12 Naf97de267c97459d83b64c85897eab25
    13 Nce34c9be254e478fa945bc4215a5c815
    14 Nd813094b13eb42c0a4f7dbc3e17a0cc1
    15 anzsrc-for:06
    16 anzsrc-for:0601
    17 schema:author N242e177612f04f1299ad6a04fc95bb09
    18 schema:datePublished 1987-07
    19 schema:datePublishedReg 1987-07-01
    20 schema:description Most mitochondrial proteins are encoded by nuclear genes and are synthesized as precursors containing a presequence at the N terminus. In yeast and in mammalian cells, the function of the presequence in mitochondrial targeting has been revealed by chimaeric gene studies. Fusion of a mitochondrial presequence to a foreign protein coding sequence enables the protein to be imported into mitochondria in vitro as well as in vivo. Whether plant mitochondrial presequences function in the same way has been unknown. We have previously isolated and characterized a nuclear gene (atp2-1) from Nicotiana plumbaginifolia that encodes the beta-subunit of the mitochondrial ATP synthase. We have constructed a chimaeric gene comprising a putative atp2-1 presequence fused to the bacterial chloramphenicol acetyltransferase (CAT) coding sequence and introduced it into the tobacco genome. We report here that a segment of 90 amino acids of the N terminus of the beta-subunit precursor is sufficient for the specific targeting of the CAT protein to mitochondria in transgenic plants. Our results demonstrate a high specificity for organelle targeting in plant cells.
    21 schema:genre research_article
    22 schema:inLanguage en
    23 schema:isAccessibleForFree false
    24 schema:isPartOf N8915e9969ff14a7c905c8ebeaf80f34b
    25 Nf330e930b34d427192b6cf760ed17ea6
    26 sg:journal.1018957
    27 schema:name Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants
    28 schema:pagination 340-342
    29 schema:productId N35035241449840418fd4008a95ddd8e0
    30 N6ffc5dbc0cd54a9ebc9ab179bf49997a
    31 N7e5eca8852094ba3adcf7b3ea99f23ef
    32 N9197340547ef468aa982d8865a4844eb
    33 N97312e3c43cd4aaea8eb55cdb4a86242
    34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051691717
    35 https://doi.org/10.1038/328340a0
    36 schema:sdDatePublished 2019-04-10T18:08
    37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    38 schema:sdPublisher N75151b3c7eea4ba28c85c52cf1251fdc
    39 schema:url http://www.nature.com/articles/328340a0
    40 sgo:license sg:explorer/license/
    41 sgo:sdDataset articles
    42 rdf:type schema:ScholarlyArticle
    43 N019f7049e7864812a7f4e4323abb3765 rdf:first sg:person.01143656367.83
    44 rdf:rest Nc13bea0fb283443ca1bf4a0e2accb9af
    45 N0c1e43a241ba4a4c963b4ef9d6389069 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    46 schema:name Mitochondria
    47 rdf:type schema:DefinedTerm
    48 N242e177612f04f1299ad6a04fc95bb09 rdf:first sg:person.01156422660.48
    49 rdf:rest N762d7a9578cf408992676e929ea26089
    50 N2c269912dd1f40719b4ee53c11f59012 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    51 schema:name Plants, Toxic
    52 rdf:type schema:DefinedTerm
    53 N30eab6d02de24684a832fada10e29cd7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    54 schema:name Base Sequence
    55 rdf:type schema:DefinedTerm
    56 N35035241449840418fd4008a95ddd8e0 schema:name doi
    57 schema:value 10.1038/328340a0
    58 rdf:type schema:PropertyValue
    59 N369f85c2383f4088bb23bc844ff6646c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    60 schema:name Promoter Regions, Genetic
    61 rdf:type schema:DefinedTerm
    62 N3f2a89a24d2145b1a9d8d933a8881745 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    63 schema:name Plants
    64 rdf:type schema:DefinedTerm
    65 N51588927446248d08bfd27f5a5a73ff5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    66 schema:name Chloramphenicol O-Acetyltransferase
    67 rdf:type schema:DefinedTerm
    68 N65dd53eb71fd4614970c089607203488 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    69 schema:name Malate Dehydrogenase
    70 rdf:type schema:DefinedTerm
    71 N6ffc5dbc0cd54a9ebc9ab179bf49997a schema:name readcube_id
    72 schema:value f63ba98749dd218869a84891396950c6ef262d928540c94777e92b86a9892595
    73 rdf:type schema:PropertyValue
    74 N75151b3c7eea4ba28c85c52cf1251fdc schema:name Springer Nature - SN SciGraph project
    75 rdf:type schema:Organization
    76 N75b88a11fd564b509101610aab61456b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Genes
    78 rdf:type schema:DefinedTerm
    79 N762d7a9578cf408992676e929ea26089 rdf:first sg:person.012421646303.49
    80 rdf:rest Nbfe9375f33994b93a3f01ddcbaa61a4c
    81 N7e5eca8852094ba3adcf7b3ea99f23ef schema:name dimensions_id
    82 schema:value pub.1051691717
    83 rdf:type schema:PropertyValue
    84 N82b84736930747abbb32f3dedc11d108 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Amino Acid Sequence
    86 rdf:type schema:DefinedTerm
    87 N8915e9969ff14a7c905c8ebeaf80f34b schema:volumeNumber 328
    88 rdf:type schema:PublicationVolume
    89 N8c1bc09cd0c34dba9a0df12fb11aacef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Chloroplasts
    91 rdf:type schema:DefinedTerm
    92 N8c818910209644b7ab5e95bf3d7454c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Cloning, Molecular
    94 rdf:type schema:DefinedTerm
    95 N9197340547ef468aa982d8865a4844eb schema:name pubmed_id
    96 schema:value 3474528
    97 rdf:type schema:PropertyValue
    98 N97312e3c43cd4aaea8eb55cdb4a86242 schema:name nlm_unique_id
    99 schema:value 0410462
    100 rdf:type schema:PropertyValue
    101 Naf97de267c97459d83b64c85897eab25 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Genes, Bacterial
    103 rdf:type schema:DefinedTerm
    104 Nbfe9375f33994b93a3f01ddcbaa61a4c rdf:first sg:person.011566154641.05
    105 rdf:rest N019f7049e7864812a7f4e4323abb3765
    106 Nc13bea0fb283443ca1bf4a0e2accb9af rdf:first sg:person.01255377003.26
    107 rdf:rest rdf:nil
    108 Nce34c9be254e478fa945bc4215a5c815 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    109 schema:name Acetyltransferases
    110 rdf:type schema:DefinedTerm
    111 Nd813094b13eb42c0a4f7dbc3e17a0cc1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    112 schema:name Tobacco
    113 rdf:type schema:DefinedTerm
    114 Nf330e930b34d427192b6cf760ed17ea6 schema:issueNumber 6128
    115 rdf:type schema:PublicationIssue
    116 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    117 schema:name Biological Sciences
    118 rdf:type schema:DefinedTerm
    119 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    120 schema:name Biochemistry and Cell Biology
    121 rdf:type schema:DefinedTerm
    122 sg:journal.1018957 schema:issn 0090-0028
    123 1476-4687
    124 schema:name Nature
    125 rdf:type schema:Periodical
    126 sg:person.01143656367.83 schema:familyName Aoyagi
    127 schema:givenName Kazuko
    128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01143656367.83
    129 rdf:type schema:Person
    130 sg:person.01156422660.48 schema:familyName Boutry
    131 schema:givenName Marc
    132 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01156422660.48
    133 rdf:type schema:Person
    134 sg:person.011566154641.05 schema:familyName Poulsen
    135 schema:givenName Carsten
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011566154641.05
    137 rdf:type schema:Person
    138 sg:person.012421646303.49 schema:familyName Nagy
    139 schema:givenName Ferenc
    140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012421646303.49
    141 rdf:type schema:Person
    142 sg:person.01255377003.26 schema:familyName Chua
    143 schema:givenName Nam-Hai
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255377003.26
    145 rdf:type schema:Person
     




    Preview window. Press ESC to close (or click here)


    ...