T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-06

AUTHORS

Willi Schäfer, Andrea Görz, Günter Kahl

ABSTRACT

Agrobacterium tumefaciens induces tumorous proliferations on dicotyledonous plants by transferring a specific region (T-DNA) of its tumour-inducing (Ti) plasmid into wound-activated plant cells, where it becomes integrated into nuclear DNA1 and expressed2. The vir region of the Ti plasmid is essential for T-DNA transfer3,4 and is induced by specific wound substances from plants5–9. Agrobacterium is therefore a powerful tool for gene transfer to plants, but with some exceptions10–14 this method does not seem to be applicable to the commercially important monocotyledonous crops. We report here transformation of the monocotyledonous crop plant Dioscorea bulbifera (yam) with agrobacteria preincubated by wound substances from dicotyledons, leading to crown gall tumour formation. Moreover, we present direct evidence for the integration of T-DNA into the nuclear genome of this monocotyledonous crop. More... »

PAGES

529-532

Journal

TITLE

Nature

ISSUE

6122

VOLUME

327

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/327529a0

    DOI

    http://dx.doi.org/10.1038/327529a0

    DIMENSIONS

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