Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-06

AUTHORS

R M Palmer, A G Ferrige, S Moncada

ABSTRACT

Endothelium-derived relaxing factor (EDRF) is a labile humoral agent which mediates the action of some vasodilators. Nitrovasodilators, which may act by releasing nitric oxide (NO), mimic the effect of EDRF and it has recently been suggested by Furchgott that EDRF may be NO. We have examined this suggestion by studying the release of EDRF and NO from endothelial cells in culture. No was determined as the chemiluminescent product of its reaction with ozone. The biological activity of EDRF and of NO was measured by bioassay. The relaxation of the bioassay tissues induced by EDRF was indistinguishable from that induced by NO. Both substances were equally unstable. Bradykinin caused concentration-dependent release of NO from the cells in amounts sufficient to account for the biological activity of EDRF. The relaxations induced by EDRF and NO were inhibited by haemoglobin and enhanced by superoxide dismutase to a similar degree. Thus NO released from endothelial cells is indistinguishable from EDRF in terms of biological activity, stability, and susceptibility to an inhibitor and to a potentiator. We suggest that EDRF and NO are identical. More... »

PAGES

524-526

Journal

TITLE

Nature

ISSUE

6122

VOLUME

327

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/327524a0

    DOI

    http://dx.doi.org/10.1038/327524a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025944311

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3495737


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