High-velocity microprojectiles for delivering nucleic acids into living cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-05

AUTHORS

T. M. Klein, E. D. Wolf, R. Wu, J. C. Sanford

ABSTRACT

We report here a novel phenomenon, namely that nucleic acids can be delivered into plant cells using high-velocity microprojec-tiles. This research was conducted in the hope of circumventing some of the inherent limitations of existing methods for delivering DNA into plant cells1–6. After being accelerated, small tungsten particles (microprojectiles) pierce cell walls and membranes and enter intact plant cells without killing them. Microprojectiles were used to carry RNA or DNA into epidermal tissue of onion and these molecules were subsequently expressed genetically. This approach can therefore be used to study the transient expression of foreign genes in an intact tissue. It remains to be shown that smaller cell types, as are found in regenerable plant tissues, can be stably transformed by this method. If this proves possible, it would appear to provide a broadly applicable transformation mechanism capable of circumventing the host-range restrictions of Agrobacterium tumefaciens1, and the regeneration problems of protoplast transformation2–5. More... »

PAGES

70-73

Journal

TITLE

Nature

ISSUE

6117

VOLUME

327

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  • Method For Preparing Fertile Transgenic Corn Plants
  • Plant Udp-Galactose Epimerases
  • Swine Influenza Hemagglutinin Variants
  • Methods For Expression And Purification Of Pegylated Recombinant Human Growth Hormone Containing A Non-Naturally Encoded Keto Amino Acid
  • Gylceraldehyde-3-Phosphate Dehydrogenase Promoter And Heterologous Gene Expression
  • Glutamic Acid Decarboxylase (Gad) Chimera And Methods Of Use
  • Methods Of Producing Human Or Animal Food From Stably Transformed, Fertile Maize Plants
  • Method For Plant Regeneration Of Okra
  • Stabilized Preparations For Use In Metered Dose Inhalers
  • Isolation And Targeted Suppression Of Lignin Biosynthetic Genes
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
  • Formulations Of Human Growth Hormone Comprising A Non-Naturally Encoded Amino Acid
  • Oil Degumming Methods
  • Pollen-Mediated Plant Transformation
  • Antisense Polynucleotides To Induce Exon Skipping And Methods Of Treating Dystrophies
  • Sorghum Centromere Sequences And Minichromosomes
  • Plants Modified With Mini-Chromosomes
  • Methods And Compositions Relating To Improved Lentiviral Vectors And Their Applications
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Identification Of Terpenoid-Biosynthesis Related Regulatory Protein-Regulatory Region Associations
  • Anti-Vascular Endothelial Growth Factor Receptor-2 Chimeric Antigen Receptors And Use Of Same For The Treatment Of Cancer
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Sequence-Determined Dna Fragments Encoding Trehalose-6p Phosphatase Proteins
  • Modified Human Interferon Polypeptides With At Least One Non-Naturally Encoded Amino Acid And Their Uses
  • Transgenic Plants Expressing Civps Or Intein Modified Proteins And Related Method
  • Plants With Altered Levels Of Vegetative Starch
  • Modified Caspase Polypeptides And Uses Thereof
  • Anti-Epidermal Growth Factor Receptor Variant Iii Chimeric Antigen Receptors And Use Of Same For The Treatment Of Cancer
  • Constitutive Promoters From Poplar And Uses Thereof
  • Intein-Modified Enzymes, Their Production And Industrial Applications
  • Variant Thioesterases And Methods Of Use
  • Recombinant Expression Of Proteins From Secretory Cell Lines
  • Methods And Compositions For The Production Of Stably Transformed, Fertile Monocot Plants And Cells Thereof
  • Cell Treatment Apparatus And Method
  • Yersinia Pestis Antigens, Vaccine Compositions, And Related Methods
  • Generation Of Plants With Altered Protein, Fiber, Or Oil Content
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