HPRT-deficient (Lesch–Nyhan) mouse embryos derived from germline colonization by cultured cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-03

AUTHORS

Martin Hooper, Kate Hardy, Alan Handyside, Susan Hunter, Marilyn Monk

ABSTRACT

Embryonal stem (ES) cell lines, established in culture from peri-implantation mouse blastocysts, can colonize both the somatic and germ-cell lineages of chimaeric mice following injection into host blastocysts. Recently, ES cells with multiple integrations of retroviral sequences have been used to introduce these sequences into the germ-line of chimaeric mice, demonstrating an alternative to the microinjection of fertilized eggs for the production of transgenic mice. However, the properties of ES cells raise a unique possibility: that of using the techniques of somatic cell genetics to select cells with genetic modifications such as recessive mutations, and of introducing these mutations into the mouse germ line. Here we report the realization of this possibility by the selection in vitro of variant ES cells deficient in hypoxanthine guanine phosphoribosyl transferase (HPRT; EC 2.4.2.8), their use to produce germline chimaeras resulting in female offspring heterozygous for HPRT-deficiency, and the generation of HPRT-deficient preimplantation embryos from these females. In human males, HPRT deficiency causes Lesch-Nyhan syndrome, which is characterized by mental retardation and self-mutilation. More... »

PAGES

292-295

Journal

TITLE

Nature

ISSUE

6110

VOLUME

326

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/326292a0

    DOI

    http://dx.doi.org/10.1038/326292a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1050105118

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3821905


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