Transgenic rye plants obtained by injecting DNA into young floral tillers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-01

AUTHORS

A. de la Peña, H. Lörz, J. Schell

ABSTRACT

The most successful method currently used to produce transgenic plants is based on the ability of Argobacterium tumefaciens to infect a number of higher plant species and transfer a defined DNA fragment (T-DNA) to the genome of the infected cells1. This procedure cannot be used with the agriculturally important cereals, although preliminary data have suggested2 infection of maize seedlings by A. tumefaciens strains. Purified exogenous DNA can be taken up, integrated and expressed in cells of a variety of plant species including some cereals following direct gene transfer into isolated protoplasts3–7. However, although it is possible to grow isolated cereal protoplast into unorganized tissue (calli)5,7,8, only rice protoplasts have so far been shown to regenerate mature plants9–11. Here we report an alternative approach to transformation of cereal plants which does not involve tissue culture techniques. More... »

PAGES

274-276

References to SciGraph publications

Journal

TITLE

Nature

ISSUE

6101

VOLUME

325

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/325274a0

    DOI

    http://dx.doi.org/10.1038/325274a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002903113


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