The Tetrahymena ribozyme acts like an RNA restriction endonuclease View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-12

AUTHORS

Arthur J. Zaug, Michael D. Been, Thomas R. Cech

ABSTRACT

A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences. More... »

PAGES

429-433

Journal

TITLE

Nature

ISSUE

6096

VOLUME

324

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  • Identifiers

    URI

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    DOI

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    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3537808


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