Fluorescence detection in automated DNA sequence analysis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-06

AUTHORS

Lloyd M. Smith, Jane Z. Sanders, Robert J. Kaiser, Peter Hughes, Chris Dodd, Charles R. Connell, Cheryl Heiner, Stephen B. H. Kent, Leroy E. Hood

ABSTRACT

We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer. More... »

PAGES

674-679

Journal

TITLE

Nature

ISSUE

6071

VOLUME

321

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/321674a0

    DOI

    http://dx.doi.org/10.1038/321674a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1041827788

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3713851


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