Na-Ca exchange current in mammalian heart cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-02

AUTHORS

Junko Kimura, Akinori Noma, Hiroshi Irisawa

ABSTRACT

Electrogenic Na-Ca exchange has been known to act in the cardiac sarcolemma as a major mechanism for extruding Ca ions1–3. Ionic flux measurements in cardiac vesicles have recently suggested that the exchange ratio is probably 3 Na:l Ca (refs 4, 5), although a membrane current generated by such a process has not been isolated. Using the intracellular perfusion technique6,7 combined with the whole-cell voltage clamp8, we were able to load Na+ inside and Ca2+ outside the single ventricular cells of the guinea pig and have succeeded in recording an outward Na-Ca exchange current while blocking most other membrane currents. The current is voltage-dependent, blocked by La3+ and does not develop in the absence of intracellular free Ca2+. This report presents the first direct measurement of the cardiac Na-Ca exchange current, and should facilitate the study of Ca2+ fluxes during cardiac activity, together with various electrical changes attributable to the Na-Ca exchange9 and the testing of proposed models10–12. More... »

PAGES

596-597

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/319596a0

DOI

http://dx.doi.org/10.1038/319596a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044733136

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3945347


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