Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-01

AUTHORS

Shigekazu Nagata, Masayuki Tsuchiya, Shigetaka Asano, Yoshito Kaziro, Tatsumi Yamazaki, Osami Yamamoto, Yuichi Hirata, Naoki Kubota, Masayoshi Oheda, Hitoshi Nomura, Masayoshi Ono

ABSTRACT

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation, and G-CSF almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. Recently, Nomura et al. have established a human squamous carcinoma cell line (designated CHU-2) from a human oral cavity tumour which produces large quantities of CSF constitutively, and the CSF produced by CHU-2 cells has been purified to homogeneity from the conditioned medium. We have now determined the partial amino-acid sequence of the purified G-CSF protein, and by using oligonucleotides as probes, have isolated several clones containing G-CSF complementary DNA from the cDNA library prepared with messenger RNA from CHU-2 cells. The complete nucleotide sequences of two of these cDNAs were determined and the expression of the cDNA in monkey COS cells gave rise to a protein showing authentic G-CSF activity. Furthermore, Southern hybridization analysis of DNA from normal leukocytes and CHU-2 cells suggests that the human genome contains only one gene for G-CSF and that some rearrangement has occurred within one of the alleles of the G-CSF gene in CHU-2 cells. More... »

PAGES

415-418

Journal

TITLE

Nature

ISSUE

6052

VOLUME

319

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/319415a0

    DOI

    http://dx.doi.org/10.1038/319415a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024372940

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3484805


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/319415a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/319415a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/319415a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/319415a0'


     

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