Inhibition of the Prausnitz–Küstner reaction by an immunoglobulin ε-chain fragment synthesized in E. coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-06

AUTHORS

Raif S. Geha, Birgit Helm, Hannah Gould

ABSTRACT

The Prausnitz-Küstner (P-K) reaction is a sensitive test for the presence and activity in the skin of immunoglobulin E, an important class of immunoglobulin mediating allergic reactions. A fragment of the human myeloma ND epsilon-chain gene, encoding the second, third and fourth domains of the IgE constant region (C epsilon 2-4) was assessed here for its ability to inhibit the P-K reaction in vivo. Injection of the fragment in skin sites of healthy human adults prevented subsequent sensitization with serum containing IgE antibody to ragweed antigen. Inhibition of the P-K reaction required a 200-fold molar excess of the C epsilon fragment over the IgE present in the sensitizing serum. The efficacy of the C epsilon fragment in inhibiting the P-K reaction compared favourably with that of natural myeloma IgE (PS) in terms of both blocking concentrations and duration of the blocking effect. The inhibition of the P-K reaction by C epsilon 2-4 fragments was specific and probably caused by the saturation of IgE receptors on mast cells by the recombinant gene product. More... »

PAGES

577-578

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/315577a0

DOI

http://dx.doi.org/10.1038/315577a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039007058

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3925344


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