Molecular cloning of Ancient Egyptian mummy DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-04

AUTHORS

Svante Pääbo

ABSTRACT

Artificial mummification was practised in Egypt from approximately 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report here that one such clone contains two members of the Alu family of human repetitive DNA sequences, as detected by DNA hybridizations and nucleotide sequencing. These analyses show that substantial pieces of mummy DNA (3.4 kilobases) can be cloned and that the DNA fragments seem to contain little or no modifications introduced postmortem. More... »

PAGES

644-645

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/314644a0

DOI

http://dx.doi.org/10.1038/314644a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044179528

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3990798


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mummies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Repetitive Sequences, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "familyName": "P\u00e4\u00e4bo", 
        "givenName": "Svante", 
        "id": "sg:person.01330431426.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330431426.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0076-6879(80)65059-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008420927"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(77)90052-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010609967"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(78)90262-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011837579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(81)90219-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013078435"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-583x(84)90501-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013834628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-583x(84)90501-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013834628"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/10520295509114479", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026014347"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/7.8.2115", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027093851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0190-9622(81)70003-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041327824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/312282a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042967662", 
          "https://doi.org/10.1038/312282a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/224325b0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049094186", 
          "https://doi.org/10.1038/224325b0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050786151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050786151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.349693", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062617228"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.6281889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062636024"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1985-04", 
    "datePublishedReg": "1985-04-01", 
    "description": "Artificial mummification was practised in Egypt from approximately 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report here that one such clone contains two members of the Alu family of human repetitive DNA sequences, as detected by DNA hybridizations and nucleotide sequencing. These analyses show that substantial pieces of mummy DNA (3.4 kilobases) can be cloned and that the DNA fragments seem to contain little or no modifications introduced postmortem.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1038/314644a0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0090-0028", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6012", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "314"
      }
    ], 
    "name": "Molecular cloning of Ancient Egyptian mummy DNA", 
    "pagination": "644-645", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d27142546e126b59c98bc20cafac8f3ee7a50abfb1727283267b2c27b72be477"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "3990798"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0410462"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/314644a0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044179528"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/314644a0", 
      "https://app.dimensions.ai/details/publication/pub.1044179528"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000426.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://www.nature.com/articles/314644a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/314644a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/314644a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/314644a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/314644a0'


 

This table displays all metadata directly associated to this object as RDF triples.

130 TRIPLES      21 PREDICATES      48 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/314644a0 schema:about N103970b5159b4dae8e6c2f4a914eb881
2 N3eaf5b9272f341eda40ad6852cfaf14f
3 N82d6c3ee03b14348922cee44d9209096
4 N8f67889b5c7b4fa9b5e4740cba7716a1
5 N9edd3cec3ba94af09006c4c315b04615
6 Ne1f926021fe94e8d933c3c35ca89229f
7 anzsrc-for:06
8 anzsrc-for:0604
9 schema:author Nb1bd00f11c2540c0917a5127ffa3f488
10 schema:citation sg:pub.10.1038/224325b0
11 sg:pub.10.1038/312282a0
12 https://doi.org/10.1016/0003-2697(78)90262-2
13 https://doi.org/10.1016/0022-2836(77)90052-3
14 https://doi.org/10.1016/0022-2836(81)90219-9
15 https://doi.org/10.1016/0168-583x(84)90501-9
16 https://doi.org/10.1016/0378-1119(82)90015-4
17 https://doi.org/10.1016/s0076-6879(80)65059-9
18 https://doi.org/10.1016/s0190-9622(81)70003-3
19 https://doi.org/10.1093/nar/7.8.2115
20 https://doi.org/10.1126/science.349693
21 https://doi.org/10.1126/science.6281889
22 https://doi.org/10.3109/10520295509114479
23 schema:datePublished 1985-04
24 schema:datePublishedReg 1985-04-01
25 schema:description Artificial mummification was practised in Egypt from approximately 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report here that one such clone contains two members of the Alu family of human repetitive DNA sequences, as detected by DNA hybridizations and nucleotide sequencing. These analyses show that substantial pieces of mummy DNA (3.4 kilobases) can be cloned and that the DNA fragments seem to contain little or no modifications introduced postmortem.
26 schema:genre research_article
27 schema:inLanguage en
28 schema:isAccessibleForFree false
29 schema:isPartOf N02de4d8250dc4d7a9d8bde7c206274ba
30 N3df7e902a0a947688974c5fe9cb510b3
31 sg:journal.1018957
32 schema:name Molecular cloning of Ancient Egyptian mummy DNA
33 schema:pagination 644-645
34 schema:productId N43c942d87104448eaa8b498c470a6f46
35 N449d01cc30cd4437964ba72bda3b5ce8
36 N61f50a957fe3405f9728ca6f24b14a40
37 N885a96e139bb42118521fb43c9518359
38 Nec4e11d70f164cbe899c36c4cd8c5820
39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044179528
40 https://doi.org/10.1038/314644a0
41 schema:sdDatePublished 2019-04-10T23:12
42 schema:sdLicense https://scigraph.springernature.com/explorer/license/
43 schema:sdPublisher N01e58cc50e264895a4cc976660d3ef20
44 schema:url http://www.nature.com/articles/314644a0
45 sgo:license sg:explorer/license/
46 sgo:sdDataset articles
47 rdf:type schema:ScholarlyArticle
48 N01e58cc50e264895a4cc976660d3ef20 schema:name Springer Nature - SN SciGraph project
49 rdf:type schema:Organization
50 N02de4d8250dc4d7a9d8bde7c206274ba schema:issueNumber 6012
51 rdf:type schema:PublicationIssue
52 N103970b5159b4dae8e6c2f4a914eb881 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Humans
54 rdf:type schema:DefinedTerm
55 N3df7e902a0a947688974c5fe9cb510b3 schema:volumeNumber 314
56 rdf:type schema:PublicationVolume
57 N3eaf5b9272f341eda40ad6852cfaf14f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Repetitive Sequences, Nucleic Acid
59 rdf:type schema:DefinedTerm
60 N43c942d87104448eaa8b498c470a6f46 schema:name dimensions_id
61 schema:value pub.1044179528
62 rdf:type schema:PropertyValue
63 N449d01cc30cd4437964ba72bda3b5ce8 schema:name doi
64 schema:value 10.1038/314644a0
65 rdf:type schema:PropertyValue
66 N61f50a957fe3405f9728ca6f24b14a40 schema:name readcube_id
67 schema:value d27142546e126b59c98bc20cafac8f3ee7a50abfb1727283267b2c27b72be477
68 rdf:type schema:PropertyValue
69 N82d6c3ee03b14348922cee44d9209096 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name DNA
71 rdf:type schema:DefinedTerm
72 N885a96e139bb42118521fb43c9518359 schema:name pubmed_id
73 schema:value 3990798
74 rdf:type schema:PropertyValue
75 N8f67889b5c7b4fa9b5e4740cba7716a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Skin
77 rdf:type schema:DefinedTerm
78 N9edd3cec3ba94af09006c4c315b04615 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Cloning, Molecular
80 rdf:type schema:DefinedTerm
81 Nb1bd00f11c2540c0917a5127ffa3f488 rdf:first sg:person.01330431426.48
82 rdf:rest rdf:nil
83 Ne1f926021fe94e8d933c3c35ca89229f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Mummies
85 rdf:type schema:DefinedTerm
86 Nec4e11d70f164cbe899c36c4cd8c5820 schema:name nlm_unique_id
87 schema:value 0410462
88 rdf:type schema:PropertyValue
89 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
90 schema:name Biological Sciences
91 rdf:type schema:DefinedTerm
92 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
93 schema:name Genetics
94 rdf:type schema:DefinedTerm
95 sg:journal.1018957 schema:issn 0090-0028
96 1476-4687
97 schema:name Nature
98 rdf:type schema:Periodical
99 sg:person.01330431426.48 schema:familyName Pääbo
100 schema:givenName Svante
101 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01330431426.48
102 rdf:type schema:Person
103 sg:pub.10.1038/224325b0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049094186
104 https://doi.org/10.1038/224325b0
105 rdf:type schema:CreativeWork
106 sg:pub.10.1038/312282a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042967662
107 https://doi.org/10.1038/312282a0
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/0003-2697(78)90262-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011837579
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/0022-2836(77)90052-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010609967
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1016/0022-2836(81)90219-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013078435
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1016/0168-583x(84)90501-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013834628
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1016/0378-1119(82)90015-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786151
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1016/s0076-6879(80)65059-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008420927
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1016/s0190-9622(81)70003-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041327824
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1093/nar/7.8.2115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027093851
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1126/science.349693 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062617228
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1126/science.6281889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062636024
128 rdf:type schema:CreativeWork
129 https://doi.org/10.3109/10520295509114479 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026014347
130 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...