Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-02

AUTHORS

Joan T. Odell, Ferenc Nagy, Nam-Hai Chua

ABSTRACT

Although promoter regions for many plant nuclear genes have been sequenced, identification of the active promoter sequence has been carried out only for the octopine synthase promoter. That analysis was of callus tissue and made use of an enzyme assay. We have analysed the effects of 5' deletions in a plant viral promoter in tobacco callus as well as in regenerated plants, including different plant tissues. We assayed the RNA transcription product which allows a more direct assessment of deletion effects. The cauliflower mosaic virus (CaMV) 35S promoter provides a model plant nuclear promoter system, as its double-strand DNA genome is transcribed by host nuclear RNA polymerase II from a CaMV minichromosome. Sequences extending to -46 were sufficient for accurate transcription initiation whereas the region between -46 and -105 increased greatly the level of transcription. The 35S promoter showed no tissue-specificity of expression. More... »

PAGES

810-812

Journal

TITLE

Nature

ISSUE

6005

VOLUME

313

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/313810a0

    DOI

    http://dx.doi.org/10.1038/313810a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035513087

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3974711


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