Cloning and expression of murine interleukin-1 cDNA in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-11

AUTHORS

Peter T. Lomedico, Ueli Gubler, Christopher P. Hellmann, Mitchell Dukovich, Judith G. Giri, Yu-Ching E. Pan, Kenneth Collier, Raina Semionow, Anne O. Chua, Steven B. Mizel

ABSTRACT

Interleukin-1 (IL-1), a peptide hormone produced by activated macrophages, possesses the ability to modulate the proliferation, maturation and functional activation of a broad spectrum of cell types and may play a major role in the initiation and amplification of immune and inflammatory responses through its action on these diverse cell populations. IL-1 exhibits microheterogeneity in terms of its relative molecular mass (Mr, 13,000-19,000) and charge properties, and although murine IL-1 has been purified and some of its basic structure-function relationships have been elucidated, it has proved difficult to prepare sufficient amounts of IL-1 for direct and detailed sequence and structural studies. Here we report the cloning, sequence analysis and expression of murine IL-1 cDNA in Escherichia coli. The IL-1 cDNA codes for a polypeptide precursor of 270 amino acids. Biologically active IL-1 was produced in E. coli by expressing the carboxy-terminal 156 amino acids of the IL-1 precursor. More... »

PAGES

458-462

Journal

TITLE

Nature

ISSUE

5993

VOLUME

312

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/312458a0

DOI

http://dx.doi.org/10.1038/312458a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044294426

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6209582


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