Direct observation of motion of single F-actin filaments in the presence of myosin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-01

AUTHORS

Toshio Yanagida, Michiyuki Nakase, Katsumi Nishiyama, Fumio Oosawa

ABSTRACT

Actin is found in almost all kinds of non-muscle cells where it is thought to have an important role in cell motility. A proper understanding of that role will only be possible when reliable in vitro systems are available for investigating the interaction of cellular actin and myosin. A start has been made on several systems1–4, most recently by Sheetz and Spudich who demonstrated unidirectional movement of HMM-coated beads along F-actin cables on arrays of chloroplasts exposed by dissection of a Nitella cell5. As an alternative approach, we report here the direct observation by fluorescence microscopy of the movements of single F-actin filaments interacting with soluble myosin fragments energized by Mg2+-ATP. More... »

PAGES

58-60

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/307058a0

DOI

http://dx.doi.org/10.1038/307058a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053227057

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6537825


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