A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-05

AUTHORS

A. Hoekema, P. R. Hirsch, P. J. J. Hooykaas, R. A. Schilperoort

ABSTRACT

The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown-gall tumours after infection of wounded dicotyledonous plants. Large plasmids (Ti-plasmids) are responsible for the oncogenicity of the bacterium1–3. Crown-gall tumours contain a DNA segment, called the T-DNA, which is homologous with a defined part of the Ti-plasmid present in the tumour-inducing bacterium, and is stably integrated into the plant genome4–7. Apart from the T-DNA another region of the Ti-plasmid-called the vir-region, is essential for tumour induction8–11. We report here the interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon. An A. tumefaciens strain harbouring both plasmids has a normal tumour-inducing capacity, although neither plasmid is functional alone. With this approach, the T-DNA on one plasmid can, because of its size, be easily genetically manipulated using Escherichia coli as a host. Transfer of this plasmid into an A. tumefaciens strain harbouring the plasmid with the vir-region allows introduction of the manipulated T-DNA into plant cells. In this way, sophisticated binary vector systems for plant genetic engineering can be developed. More... »

PAGES

179-180

Journal

TITLE

Nature

ISSUE

5913

VOLUME

303

Related Patents

  • Transformation Of Cotton Plants
  • Transformation System With Ti Or Ri Plasmid
  • Transformed Cotton Plants
  • Transformed Cotton Plants
  • Cyclopropane Fatty Acid Expression In Plants
  • Methods And Compositions For Regulated Transcription And Expression Of Heterologous Genes
  • Methods And Compositions For Regulated Transcription And Expression Of Heterologous Genes
  • Transformation Vectors Allowing Expression Of Foreign Polypeptide Endotoxins From Bacillus Thuringiensis In Plants
  • Chitinase-Producing Bacteria
  • Method For Improving Transformation Efficiency Using Powder
  • A Process For The Incorporation Of Foreign Dna Into The Genome Of Dicotyledonous Plants; Agrobacterium Tumefaciens Bacteria And A Process For The Production Thereof; Plants And Plant Cells With Modified Genetic Properties; A Process For The Preparation Of Chemical And/Or Pharmaceutical Products
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Method Of Large Scale Mutagenesis In Tomato Plants
  • Agrobacterium Bacterium To Be Used In Plant Transformation Method
  • Methods For Decreasing Linolenic Acid Content In Seeds From Transgenic Plants Containing A Mutant Delta 15 Desaturase
  • Transformation Vectors Allowing Expression Of Foreign Polypeptide Endotoxins From Bacillus Thuringiensis In Plants
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Agrobacterium Bacterium To Be Used In Plant Transformation Method
  • Method For Controlling Herbicide-Resistant Plants
  • Recombinant Dna Constructs And Methods For Controlling Gene Expression
  • Invertebrate Micrornas
  • Process For Introducing Foreign Dna Into Genome Of Plants
  • Methods And Compositions For Delaying Senescence And Improving Disease Tolerance And Yield In Plants
  • Transgenic Plants With Reduced Level Of Saturated Fatty Acid And Methods For Making Them
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Method And Compositions For The Production Of Transgenic Plants
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Tobacco Inbred Plants Ncbex1f, Ncbex1ms, And Nc Ex90
  • Compositions And Methods For Controlling Leptinotarsa
  • Down-Regulation And Silencing Of Allergen Genes In Transgenic Peanut Seeds
  • Production Of Polyketides In Plants
  • Cotton Plant Promoters
  • Transformation Vectors Allowing Expression Of Truncated Bt2 Endotoxins In Plants
  • Endotoxins
  • Transformation Vectors Allowing Expression Of Foreign Polypeptide Endotoxins In Plants
  • Polynucleotide Molecules For Gene Regulation In Plants
  • Compositions And Methods For Controlling Arthropod Parasite And Pest Infestations
  • Gene For A Dof Transcription Factor Capable Of Altering The Size And Stature Of A Plant
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • A Gene From Solanum Bulbocastanum Conferring Resistance To Phytophthora Infestans
  • Constitutive Promoter From Oil Palm
  • Backbone-Free Low Transgene Copy Transgenic Plants
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Use Of Non-Agrobacterium Bacterial Species For Plant Transformation
  • Methods And Compositions For Plant Pest Control
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Plants Comprising Pathogen Effector Constructs
  • Methods And Compositions For Plant Pest Control
  • Regulatory Sequences For Regulation Of Gene Expression In Plants And Other Organisms, And Compositions, Products And Methods Related Thereto
  • Process For The Incorporation Of Foreign Dna Into The Genome Of Dicotyledonous Plants
  • Method For Increasing The Ratio Of P-Hydroxybenzoic Acid Ester Glucoside To Total P-Hydroxybenzoic Acid Glucose Conjugates In P-Hydroxybenzoic Acid-Producing Microorganisms And Green Plant Cells
  • Method Of Protecting Plants By Introducing A Gene Coded For A Protein Which Enhances Virus Infection Of Host Insects
  • A Process For The Incorporation Of Foreign Dna Into The Genome Of Dicotyledonous Plants; Agrobacterium Tumefaciens Bacteria And A Process For The Production Thereof
  • Plant Promoter And Method For Gene Expression Using Said Promoter
  • A Method For Producing Intact Plants Containing Foreign Dna.
  • Genetic Engineering Of Cotton Plants And Lines
  • Recombinant Dna Constructs Encoding Ribonuclease Cleavage Blockers And Methods For Modulating Expression Of A Target Gene
  • Phased Small Rnas
  • Methods And Compositions For Increasing Invertase Activity In Plants
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Methods And Compositions For Weed Control
  • Transformation Of Cotton Plants
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes
  • Methods And Compositions For Weed Control
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Disarmed Agrobacterium Strains, Ri-Plasmids, And Methods Of Transformation Based Thereon
  • Compositions And Methods For Controlling Leptinotarsa
  • Disarmed Agrobacterium Strains, Ri-Plasmids, And Methods Of Transformation Based Thereon
  • A Method For Producing Intact Plants Containing Foreign Dna
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Tobacco Inbred And Hybrid Plants And Uses Thereof
  • Activation Of The Arabidopsis Hypertall (Hyt1/Yucca6) Locus Affects Several Auxin Mediated Responses
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Chitinase-Producing Plants
  • Transformation And Foreign Gene Expression With Plant Species
  • Isolated Dsrna Molecules And Methods Of Using Same For Silencing Target Molecules Of Interest
  • Expression Modulating Sequences
  • Chitinase-Producing Bacteria And Plants
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Transgenic Plants Expressing Glk1 And Cca1 Having Increased Nitrogen Assimilation Capacity
  • Modified Gene Silencing
  • Methods And Compositions For Weed Control
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Compositions And Methods For Controlling Leptinotarsa
  • Tobacco Inbred Plants K326 Src, Cms K326 Src, K346 Src, Cms K346 Src, Nc1562-1 Src, Nctg-61 Src, Cms Nctg-61 Src And Hybrid Nc196 Src
  • Compositions And Methods For Minimizing Nornicotine Synthesis In Tobacco
  • Chitinase-Producing Plants
  • Plant Micrornas And Methods Of Use Thereof
  • Recombinant Dna Constructs And Methods For Controlling Gene Expression
  • Recombinant Dna Constructs And Methods For Controlling Gene Expression
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Nicotiana Nucleic Acid Molecules And Uses Thereof
  • Tobacco Inbred Plants Albex1f And Albex1ms
  • Plant Protease
  • A Process For The Incorporation Of Foreign Dna Into The Genome Of Monocotyledonous Plants
  • Decreasing Nitrite Content In Tobacco Via Expression Of A Nitrite Reductase
  • Tobacco Inbred And Hybrid Plants And Tobacco Products Made Thereof
  • Genetically Engineered Modification Of Potato To Form Amylopectin-Type Starch
  • Method For Producing Intact Plants Containing Foreign Dna
  • Genetically Engineered Modification Of Potato To Form Amylopectin-Type Starch
  • Tobacco Inbred Plants Albex1f And Albex1ms
  • Plant Protease
  • Tobacco Nicotine Demethylase Genomic Clone And Uses Thereof
  • Methods Of Affecting Nitrogen Assimilation In Plants
  • Cloning Of Cytochrome P450 Genes From Nicotiana
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/303179a0

    DOI

    http://dx.doi.org/10.1038/303179a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018421874


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "familyName": "Hoekema", 
            "givenName": "A.", 
            "id": "sg:person.0742422543.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742422543.21"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Hirsch", 
            "givenName": "P. R.", 
            "type": "Person"
          }, 
          {
            "familyName": "Hooykaas", 
            "givenName": "P. J. J.", 
            "id": "sg:person.014770643204.78", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014770643204.78"
            ], 
            "type": "Person"
          }, 
          {
            "familyName": "Schilperoort", 
            "givenName": "R. A.", 
            "id": "sg:person.01313574145.95", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313574145.95"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00270630", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000236162", 
              "https://doi.org/10.1007/bf00270630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270630", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000236162", 
              "https://doi.org/10.1007/bf00270630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2744(78)90363-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002663215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2744(78)90363-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002663215"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(82)90255-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005203441"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(80)90153-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006086175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(80)90153-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006086175"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/275104a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006573233", 
              "https://doi.org/10.1038/275104a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/275104a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006573233", 
              "https://doi.org/10.1038/275104a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(74)80011-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011473143"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/252169a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013100174", 
              "https://doi.org/10.1038/252169a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(81)90069-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014004086"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(80)80049-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016541918"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/283216a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017777265", 
              "https://doi.org/10.1038/283216a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(77)90043-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036529145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(82)90071-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038902421"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(81)90070-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043730421"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(81)90146-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044580637"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(78)90043-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045259484"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(80)90083-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053048819"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074836803", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081334027", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081645437", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081646039", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1983-05", 
        "datePublishedReg": "1983-05-01", 
        "description": "The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown-gall tumours after infection of wounded dicotyledonous plants. Large plasmids (Ti-plasmids) are responsible for the oncogenicity of the bacterium1\u20133. Crown-gall tumours contain a DNA segment, called the T-DNA, which is homologous with a defined part of the Ti-plasmid present in the tumour-inducing bacterium, and is stably integrated into the plant genome4\u20137. Apart from the T-DNA another region of the Ti-plasmid-called the vir-region, is essential for tumour induction8\u201311. We report here the interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon. An A. tumefaciens strain harbouring both plasmids has a normal tumour-inducing capacity, although neither plasmid is functional alone. With this approach, the T-DNA on one plasmid can, because of its size, be easily genetically manipulated using Escherichia coli as a host. Transfer of this plasmid into an A. tumefaciens strain harbouring the plasmid with the vir-region allows introduction of the manipulated T-DNA into plant cells. In this way, sophisticated binary vector systems for plant genetic engineering can be developed.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1038/303179a0", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1018957", 
            "issn": [
              "0090-0028", 
              "1476-4687"
            ], 
            "name": "Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5913", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "303"
          }
        ], 
        "name": "A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid", 
        "pagination": "179-180", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "57c6426395b658574692a6ce61384d41e26b019712ea7427077bedea5475d16d"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1038/303179a0"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1018421874"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1038/303179a0", 
          "https://app.dimensions.ai/details/publication/pub.1018421874"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:24", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000424.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://www.nature.com/articles/303179a0"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/303179a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/303179a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/303179a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/303179a0'


     

    This table displays all metadata directly associated to this object as RDF triples.

    137 TRIPLES      21 PREDICATES      48 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1038/303179a0 schema:about anzsrc-for:06
    2 anzsrc-for:0605
    3 schema:author Ne9d0545f99174d519425f6d4a49855d0
    4 schema:citation sg:pub.10.1007/bf00270630
    5 sg:pub.10.1038/252169a0
    6 sg:pub.10.1038/275104a0
    7 sg:pub.10.1038/283216a0
    8 https://app.dimensions.ai/details/publication/pub.1074836803
    9 https://app.dimensions.ai/details/publication/pub.1081334027
    10 https://app.dimensions.ai/details/publication/pub.1081645437
    11 https://app.dimensions.ai/details/publication/pub.1081646039
    12 https://doi.org/10.1016/0005-2744(78)90363-7
    13 https://doi.org/10.1016/0092-8674(77)90043-5
    14 https://doi.org/10.1016/0092-8674(82)90255-0
    15 https://doi.org/10.1016/0147-619x(78)90043-4
    16 https://doi.org/10.1016/0147-619x(80)90083-9
    17 https://doi.org/10.1016/0147-619x(81)90069-x
    18 https://doi.org/10.1016/0147-619x(81)90070-6
    19 https://doi.org/10.1016/0147-619x(82)90071-3
    20 https://doi.org/10.1016/0378-1119(80)90153-5
    21 https://doi.org/10.1016/0378-1119(81)90146-3
    22 https://doi.org/10.1016/s0022-2836(74)80011-2
    23 https://doi.org/10.1016/s0022-2836(75)80083-0
    24 https://doi.org/10.1016/s0092-8674(80)80049-3
    25 schema:datePublished 1983-05
    26 schema:datePublishedReg 1983-05-01
    27 schema:description The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown-gall tumours after infection of wounded dicotyledonous plants. Large plasmids (Ti-plasmids) are responsible for the oncogenicity of the bacterium1–3. Crown-gall tumours contain a DNA segment, called the T-DNA, which is homologous with a defined part of the Ti-plasmid present in the tumour-inducing bacterium, and is stably integrated into the plant genome4–7. Apart from the T-DNA another region of the Ti-plasmid-called the vir-region, is essential for tumour induction8–11. We report here the interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon. An A. tumefaciens strain harbouring both plasmids has a normal tumour-inducing capacity, although neither plasmid is functional alone. With this approach, the T-DNA on one plasmid can, because of its size, be easily genetically manipulated using Escherichia coli as a host. Transfer of this plasmid into an A. tumefaciens strain harbouring the plasmid with the vir-region allows introduction of the manipulated T-DNA into plant cells. In this way, sophisticated binary vector systems for plant genetic engineering can be developed.
    28 schema:genre research_article
    29 schema:inLanguage en
    30 schema:isAccessibleForFree false
    31 schema:isPartOf Nafa5709d7bf84628ba6d7701d079783f
    32 Nc4905e8b1b4f48bb92c63f6122be53fd
    33 sg:journal.1018957
    34 schema:name A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid
    35 schema:pagination 179-180
    36 schema:productId N5cd11e703e3c419ab7dd9b05ce44f124
    37 Na9f7f496c105424380a634cf60259ca7
    38 Nf1d1b2d51fa945e1a90225e18c708334
    39 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018421874
    40 https://doi.org/10.1038/303179a0
    41 schema:sdDatePublished 2019-04-10T21:24
    42 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    43 schema:sdPublisher Nebe4524789694a479e78273fbb4324c7
    44 schema:url http://www.nature.com/articles/303179a0
    45 sgo:license sg:explorer/license/
    46 sgo:sdDataset articles
    47 rdf:type schema:ScholarlyArticle
    48 N55d76f1ac68a4e30a72433290b2e50d6 rdf:first N68fd435044544bb3bcc9207e365a9578
    49 rdf:rest N98c620f279c943a1938d91c59045e4d4
    50 N5cd11e703e3c419ab7dd9b05ce44f124 schema:name doi
    51 schema:value 10.1038/303179a0
    52 rdf:type schema:PropertyValue
    53 N68fd435044544bb3bcc9207e365a9578 schema:familyName Hirsch
    54 schema:givenName P. R.
    55 rdf:type schema:Person
    56 N98c620f279c943a1938d91c59045e4d4 rdf:first sg:person.014770643204.78
    57 rdf:rest Nf076a1c22bad43fa82491b096ce73c88
    58 Na9f7f496c105424380a634cf60259ca7 schema:name dimensions_id
    59 schema:value pub.1018421874
    60 rdf:type schema:PropertyValue
    61 Nafa5709d7bf84628ba6d7701d079783f schema:volumeNumber 303
    62 rdf:type schema:PublicationVolume
    63 Nc4905e8b1b4f48bb92c63f6122be53fd schema:issueNumber 5913
    64 rdf:type schema:PublicationIssue
    65 Ne9d0545f99174d519425f6d4a49855d0 rdf:first sg:person.0742422543.21
    66 rdf:rest N55d76f1ac68a4e30a72433290b2e50d6
    67 Nebe4524789694a479e78273fbb4324c7 schema:name Springer Nature - SN SciGraph project
    68 rdf:type schema:Organization
    69 Nf076a1c22bad43fa82491b096ce73c88 rdf:first sg:person.01313574145.95
    70 rdf:rest rdf:nil
    71 Nf1d1b2d51fa945e1a90225e18c708334 schema:name readcube_id
    72 schema:value 57c6426395b658574692a6ce61384d41e26b019712ea7427077bedea5475d16d
    73 rdf:type schema:PropertyValue
    74 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    75 schema:name Biological Sciences
    76 rdf:type schema:DefinedTerm
    77 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    78 schema:name Microbiology
    79 rdf:type schema:DefinedTerm
    80 sg:journal.1018957 schema:issn 0090-0028
    81 1476-4687
    82 schema:name Nature
    83 rdf:type schema:Periodical
    84 sg:person.01313574145.95 schema:familyName Schilperoort
    85 schema:givenName R. A.
    86 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313574145.95
    87 rdf:type schema:Person
    88 sg:person.014770643204.78 schema:familyName Hooykaas
    89 schema:givenName P. J. J.
    90 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014770643204.78
    91 rdf:type schema:Person
    92 sg:person.0742422543.21 schema:familyName Hoekema
    93 schema:givenName A.
    94 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0742422543.21
    95 rdf:type schema:Person
    96 sg:pub.10.1007/bf00270630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000236162
    97 https://doi.org/10.1007/bf00270630
    98 rdf:type schema:CreativeWork
    99 sg:pub.10.1038/252169a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013100174
    100 https://doi.org/10.1038/252169a0
    101 rdf:type schema:CreativeWork
    102 sg:pub.10.1038/275104a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006573233
    103 https://doi.org/10.1038/275104a0
    104 rdf:type schema:CreativeWork
    105 sg:pub.10.1038/283216a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017777265
    106 https://doi.org/10.1038/283216a0
    107 rdf:type schema:CreativeWork
    108 https://app.dimensions.ai/details/publication/pub.1074836803 schema:CreativeWork
    109 https://app.dimensions.ai/details/publication/pub.1081334027 schema:CreativeWork
    110 https://app.dimensions.ai/details/publication/pub.1081645437 schema:CreativeWork
    111 https://app.dimensions.ai/details/publication/pub.1081646039 schema:CreativeWork
    112 https://doi.org/10.1016/0005-2744(78)90363-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002663215
    113 rdf:type schema:CreativeWork
    114 https://doi.org/10.1016/0092-8674(77)90043-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036529145
    115 rdf:type schema:CreativeWork
    116 https://doi.org/10.1016/0092-8674(82)90255-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005203441
    117 rdf:type schema:CreativeWork
    118 https://doi.org/10.1016/0147-619x(78)90043-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045259484
    119 rdf:type schema:CreativeWork
    120 https://doi.org/10.1016/0147-619x(80)90083-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053048819
    121 rdf:type schema:CreativeWork
    122 https://doi.org/10.1016/0147-619x(81)90069-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014004086
    123 rdf:type schema:CreativeWork
    124 https://doi.org/10.1016/0147-619x(81)90070-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043730421
    125 rdf:type schema:CreativeWork
    126 https://doi.org/10.1016/0147-619x(82)90071-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038902421
    127 rdf:type schema:CreativeWork
    128 https://doi.org/10.1016/0378-1119(80)90153-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006086175
    129 rdf:type schema:CreativeWork
    130 https://doi.org/10.1016/0378-1119(81)90146-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044580637
    131 rdf:type schema:CreativeWork
    132 https://doi.org/10.1016/s0022-2836(74)80011-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011473143
    133 rdf:type schema:CreativeWork
    134 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    135 rdf:type schema:CreativeWork
    136 https://doi.org/10.1016/s0092-8674(80)80049-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016541918
    137 rdf:type schema:CreativeWork
     




    Preview window. Press ESC to close (or click here)


    ...