Genetic dissection of monoamine neurotransmitter synthesis in Drosophila View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-05

AUTHORS

Margaret S. Livingstone, Bruce L. Tempel

ABSTRACT

The biogenic monoamine neurotransmitters octopamine, dopamine and serotonin have been detected in nervous tissue from many insects1. We report here that intact Drosophila melanogaster brains, when incubated with the radioactive amino acids tyrosine and tryptophan, synthesized and accumulated labelled monoamines. In two mutant strains monoamine synthesis was abnormal. The pero mutation abolishes the normal circadian rhythm2–4. Brains from pero flies, when incubated in tritiated tyrosine, accumulated one-third as much labelled octopamine as did brains from wild-type flies, but had normal dopamine and serotonin synthesis. In contrast, dopa decarboxylase (Ddc) mutations5,6 decreased dopamine and serotonin synthesis but did not affect octopamine synthesis. These results suggest that there are two different aromatic amino acid decarboxylases in Drosophila brains, one that decarboxylates L-dopa and 5-hydroxytryptophan and another that decarboxylates tyrosine. Direct measurement of L-dopa, 5-hydroxytryptophan and tyrosine decarboxylase activities in the different strains confirmed this suggestion. More... »

PAGES

67-70

References to SciGraph publications

  • 1981-10. Circadian clock phenotypes of chromosome aberrations with a breakpoint at the per locus in MOLECULAR GENETICS AND GENOMICS
  • 1972-03. Octopamine in the Lobster Nervous System in NATURE
  • 1978-01. Dopa decarboxylase from Drosophila melanogaster in MOLECULAR GENETICS AND GENOMICS
  • 1977-02. Octopamine in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/303067a0

    DOI

    http://dx.doi.org/10.1038/303067a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034021034

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6133219


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