Chloroplast DNA exists in two orientations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-01

AUTHORS

Jeffrey D. Palmer

ABSTRACT

An almost universal feature of the circular chloroplast genome is a large inverted repeat sequence, some 10–25 kilobase pairs (kb) in size, which separates the remainder of the molecule into single copy regions of ∼80 kb and 20 kb1–3. A number of physical properties—formation of head-to-head dimers1, copy-correction between the inverted repeat segments1,3,4, resistance to intramolecular recombinational loss1–3, and maintenance of a highly stable chloroplast genome resistant to rearrangement2—have been attributed to the presence of this large inverted repeat. However, one property which an inverted repeat might be expected to confer—reversal of polarity of the single copy sequences located between the repeats1—has not yet been demonstrated for the chloroplast genome. I now show that chloroplast DNA prepared from a single plant of common bean (Phaseolus vulgaris) consists of two equimolar populations of molecules differing only in the relative orientation of their single copy sequences. A model is presented to explain these results, and comparisons are made to similar cases of inversion heterogeneity in 2-micrometre plasmid DNA from yeast5,6 and in herpes simplex virus DNA7,8. More... »

PAGES

92-93

Journal

TITLE

Nature

ISSUE

5895

VOLUME

301

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/301092a0

DOI

http://dx.doi.org/10.1038/301092a0

DIMENSIONS

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