An actomyosin motor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-10

AUTHORS

M. Yano, Y. Yamamoto, H. Shimizu

ABSTRACT

Recently, two of us (M.Y. and H.S.) described an apparatus, termed the stream cell, in which a circulating flow could be induced by the interaction of heavy meromyosin (HMM) with F-actin (filamentous actin), purified from rabbit skeletal muscle1–5. Here we describe a novel system, the actomyosin motor, consisting of a rotor with F-actin attached which rotates in a specific direction, driven by the ATP-hydrolysing interaction of HMM with F-actin. This actomyosin motor can be prepared much more easily than the stream cell, and motor activity was observed with almost 100% success when the experiment was done carefully. This system represents a novel biological motor which, like the stream cell, is suitable for studying the chemo-mechanical coupling of actin and active myosin fragments. More... »

PAGES

557-559

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/299557a0

DOI

http://dx.doi.org/10.1038/299557a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009437004

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6214720


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