High frequency of gene transfer after fusion between bacteria and eukaryotic cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-01

AUTHORS

Minoo Rassoulzadegan, Bernard Binetruy, François Cuzin

ABSTRACT

Recent studies1 have demonstrated that eukaryotic simian virus 40 (SV40) genes carried in a bacterial plasmid can be directly transferred into mammalian cells in culture; after polyethylene glycol (PEG)-induced fusion with bacterial protoplasts, a fraction of the recipient cells yielded infectious virus. We have now investigated whether this procedure can also produce stable transformants. We report here that the efficiency of focus formation after transfer of either polyoma virus or SV40 early genes was at least equal to that observed after infection with virions. At high ratios of bacteria/recipient cells, all the cells were observed to express the early viral proteins 48 h after fusion. In optimal conditions, transfer by fusion thus seems to be 10- to 20-fold more efficient than DNA transfection by the Ca2+ co-precipitation technique2 for the introduction of foreign genes into eukaryotic recipients. More... »

PAGES

257-259

Journal

TITLE

Nature

ISSUE

5846

VOLUME

295

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/295257a0

    DOI

    http://dx.doi.org/10.1038/295257a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027400500

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6276764


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