Protein phosphatase-1 is involved in Xenopus oocyte maturation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-11

AUTHORS

Denise Huchon, René Ozon, Jacques G. Demaille

ABSTRACT

Two key steps in meiotic maturation of the Xenopus oocyte involve protein phosphorylation–dephosphorylation1. A burst of cyclic AMP-independent phosphorylation occurs at the time of the breakdown of the nuclear envelope, whether maturation is triggered by progesterone, by the inhibitor of cyclic AMP-dependent protein kinase (PKI)2 or by the maturation promoting factor (MPF)1,3. Also, an in ovo decrease in the level of the free catalytic (C) subunit of cyclic AMP-dependent protein kinase, induced by microinjection of pure PKI4, initiates meiotic maturation1,2,4. This suggests that MPF appears only after dephosphorylation of a phosphorylated maturation protein Mp-P4. We now show, by microinjection of pure inhibitor-1 of protein phosphatase-15, which blocks progesterone- and PKI-induced maturation but not the MPF-induced one, that protein phosphatase-1 catalyses the dephosphorylation of Mp-P. More... »

PAGES

358-359

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/294358a0

DOI

http://dx.doi.org/10.1038/294358a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010041494

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6273734


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