Molecular cloning and organization of two leghaemoglobin genomic sequences of soybean View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-02

AUTHORS

D. Sullivan, N. Brisson, B. Goodchild, D. P. S. Verma, D. Y. Thomas

ABSTRACT

The leghaemoglobins (Lb) are myoglobin-like proteins found in all nitrogen-fixing root nodules of legumes1–3. They are encoded by plant nuclear genes4 which are specifically induced and form the predominant protein in nodules developed in symbiosis with the appropriate species of Rhizobium. The Lb is located in the host-cell cytoplasm of the infected cell5 and is thought to facilitate oxygen diffusion6,7. Amino acid sequencing of the soybean Lbs has revealed at least four primary structures differing only in a few amino acids8–10. We have previously estimated about 40 copies of Lb sequences in the soybean (Glycine max L.) genome by cDNA hybridization4. To investigate Lb gene organization and function, we prepared and characterized a Lb cDNA recombinant molecule, pLb1, and used it to isolate two genomic Lb sequences from a library constructed in Charon 4. We report here that the organization of the two genomic Lb sequences is quite distinct and one of them seems to have an intervening sequence(s). Hybridization of pLb1 with genomic DNA from various tissues showed that Lb sequences are dispersed through more than 30 kilobases of genomic DNA and that there is no apparent sequence rearrangement or methylation changes following induction of Lb genes. More... »

PAGES

516-518

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/289516a0

DOI

http://dx.doi.org/10.1038/289516a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031289585


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada", 
          "id": "http://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sullivan", 
        "givenName": "D.", 
        "id": "sg:person.01367424135.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367424135.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada", 
          "id": "http://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Brisson", 
        "givenName": "N.", 
        "id": "sg:person.01023371537.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023371537.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada", 
          "id": "http://www.grid.ac/institutes/grid.14709.3b", 
          "name": [
            "Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Goodchild", 
        "givenName": "B.", 
        "type": "Person"
      }, 
      {
        "familyName": "Verma", 
        "givenName": "D. P. S.", 
        "id": "sg:person.012070664772.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012070664772.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Molecular Genetics, National Research Council, K1A 0R6, Ottawa, Canada", 
          "id": "http://www.grid.ac/institutes/grid.24433.32", 
          "name": [
            "Department of Molecular Genetics, National Research Council, K1A 0R6, Ottawa, Canada"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thomas", 
        "givenName": "D. Y.", 
        "id": "sg:person.07505723122.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07505723122.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/251074a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048652287", 
          "https://doi.org/10.1038/251074a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/282471a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004324129", 
          "https://doi.org/10.1038/282471a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1981-02", 
    "datePublishedReg": "1981-02-01", 
    "description": "The leghaemoglobins (Lb) are myoglobin-like proteins found in all nitrogen-fixing root nodules of legumes1\u20133. They are encoded by plant nuclear genes4 which are specifically induced and form the predominant protein in nodules developed in symbiosis with the appropriate species of Rhizobium. The Lb is located in the host-cell cytoplasm of the infected cell5 and is thought to facilitate oxygen diffusion6,7. Amino acid sequencing of the soybean Lbs has revealed at least four primary structures differing only in a few amino acids8\u201310. We have previously estimated about 40 copies of Lb sequences in the soybean (Glycine max L.) genome by cDNA hybridization4. To investigate Lb gene organization and function, we prepared and characterized a Lb cDNA recombinant molecule, pLb1, and used it to isolate two genomic Lb sequences from a library constructed in Charon 4. We report here that the organization of the two genomic Lb sequences is quite distinct and one of them seems to have an intervening sequence(s). Hybridization of pLb1 with genomic DNA from various tissues showed that Lb sequences are dispersed through more than 30 kilobases of genomic DNA and that there is no apparent sequence rearrangement or methylation changes following induction of Lb genes.", 
    "genre": "article", 
    "id": "sg:pub.10.1038/289516a0", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1018957", 
        "issn": [
          "0028-0836", 
          "1476-4687"
        ], 
        "name": "Nature", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5797", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "289"
      }
    ], 
    "keywords": [
      "nitrogen-fixing root nodules", 
      "genomic DNA", 
      "myoglobin-like proteins", 
      "host cell cytoplasm", 
      "amino acid sequencing", 
      "soybean genome", 
      "gene organization", 
      "Charon 4", 
      "Lb genes", 
      "root nodules", 
      "genomic sequences", 
      "Molecular cloning", 
      "methylation changes", 
      "sequence rearrangements", 
      "predominant protein", 
      "primary structure", 
      "appropriate species", 
      "recombinant molecules", 
      "sequence", 
      "protein", 
      "DNA", 
      "PLB1", 
      "genes4", 
      "genome", 
      "rhizobia", 
      "kilobases", 
      "symbiosis", 
      "cloning", 
      "genes", 
      "leghaemoglobin", 
      "species", 
      "cytoplasm", 
      "sequencing", 
      "hybridization", 
      "soybean", 
      "copies", 
      "rearrangement", 
      "induction", 
      "tissue", 
      "library", 
      "molecules", 
      "nodules", 
      "organization", 
      "function", 
      "lb", 
      "structure", 
      "changes", 
      "oxygen"
    ], 
    "name": "Molecular cloning and organization of two leghaemoglobin genomic sequences of soybean", 
    "pagination": "516-518", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031289585"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1038/289516a0"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1038/289516a0", 
      "https://app.dimensions.ai/details/publication/pub.1031289585"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:18", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_158.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1038/289516a0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/289516a0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/289516a0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/289516a0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/289516a0'


 

This table displays all metadata directly associated to this object as RDF triples.

146 TRIPLES      21 PREDICATES      76 URIs      65 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1038/289516a0 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 anzsrc-for:0604
4 schema:author N9079c97b52c94b578813d6aa143007a6
5 schema:citation sg:pub.10.1038/251074a0
6 sg:pub.10.1038/282471a0
7 schema:datePublished 1981-02
8 schema:datePublishedReg 1981-02-01
9 schema:description The leghaemoglobins (Lb) are myoglobin-like proteins found in all nitrogen-fixing root nodules of legumes1–3. They are encoded by plant nuclear genes4 which are specifically induced and form the predominant protein in nodules developed in symbiosis with the appropriate species of Rhizobium. The Lb is located in the host-cell cytoplasm of the infected cell5 and is thought to facilitate oxygen diffusion6,7. Amino acid sequencing of the soybean Lbs has revealed at least four primary structures differing only in a few amino acids8–10. We have previously estimated about 40 copies of Lb sequences in the soybean (Glycine max L.) genome by cDNA hybridization4. To investigate Lb gene organization and function, we prepared and characterized a Lb cDNA recombinant molecule, pLb1, and used it to isolate two genomic Lb sequences from a library constructed in Charon 4. We report here that the organization of the two genomic Lb sequences is quite distinct and one of them seems to have an intervening sequence(s). Hybridization of pLb1 with genomic DNA from various tissues showed that Lb sequences are dispersed through more than 30 kilobases of genomic DNA and that there is no apparent sequence rearrangement or methylation changes following induction of Lb genes.
10 schema:genre article
11 schema:isAccessibleForFree false
12 schema:isPartOf N73deb391d34a48609b1f7fc8311ca610
13 Ne2463bf8a63c4ef591643215061d0822
14 sg:journal.1018957
15 schema:keywords Charon 4
16 DNA
17 Lb genes
18 Molecular cloning
19 PLB1
20 amino acid sequencing
21 appropriate species
22 changes
23 cloning
24 copies
25 cytoplasm
26 function
27 gene organization
28 genes
29 genes4
30 genome
31 genomic DNA
32 genomic sequences
33 host cell cytoplasm
34 hybridization
35 induction
36 kilobases
37 lb
38 leghaemoglobin
39 library
40 methylation changes
41 molecules
42 myoglobin-like proteins
43 nitrogen-fixing root nodules
44 nodules
45 organization
46 oxygen
47 predominant protein
48 primary structure
49 protein
50 rearrangement
51 recombinant molecules
52 rhizobia
53 root nodules
54 sequence
55 sequence rearrangements
56 sequencing
57 soybean
58 soybean genome
59 species
60 structure
61 symbiosis
62 tissue
63 schema:name Molecular cloning and organization of two leghaemoglobin genomic sequences of soybean
64 schema:pagination 516-518
65 schema:productId N8f6e86a942fc48f0a771bc7f8f68719a
66 Na3a8bca9c3254ba38f72fa2e890b4ed2
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031289585
68 https://doi.org/10.1038/289516a0
69 schema:sdDatePublished 2022-12-01T06:18
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Neaebb351419a47bcaa44242f0c292a51
72 schema:url https://doi.org/10.1038/289516a0
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N1c5eec3faa2248138babe3a14e88875e rdf:first sg:person.07505723122.58
77 rdf:rest rdf:nil
78 N73deb391d34a48609b1f7fc8311ca610 schema:volumeNumber 289
79 rdf:type schema:PublicationVolume
80 N8f6e86a942fc48f0a771bc7f8f68719a schema:name doi
81 schema:value 10.1038/289516a0
82 rdf:type schema:PropertyValue
83 N9079c97b52c94b578813d6aa143007a6 rdf:first sg:person.01367424135.22
84 rdf:rest Ne1bd1550fe0e401c8ce0a1e0c208fbb7
85 N9c71f6c75e6347ba9873ed3a97ca328c rdf:first sg:person.012070664772.39
86 rdf:rest N1c5eec3faa2248138babe3a14e88875e
87 Na3a8bca9c3254ba38f72fa2e890b4ed2 schema:name dimensions_id
88 schema:value pub.1031289585
89 rdf:type schema:PropertyValue
90 Nb46ff524146e4c87b2c01bcd0a4dab4d schema:affiliation grid-institutes:grid.14709.3b
91 schema:familyName Goodchild
92 schema:givenName B.
93 rdf:type schema:Person
94 Nb6d2ba6d133c4c5087ffc4bcb2babbbb rdf:first Nb46ff524146e4c87b2c01bcd0a4dab4d
95 rdf:rest N9c71f6c75e6347ba9873ed3a97ca328c
96 Ne1bd1550fe0e401c8ce0a1e0c208fbb7 rdf:first sg:person.01023371537.19
97 rdf:rest Nb6d2ba6d133c4c5087ffc4bcb2babbbb
98 Ne2463bf8a63c4ef591643215061d0822 schema:issueNumber 5797
99 rdf:type schema:PublicationIssue
100 Neaebb351419a47bcaa44242f0c292a51 schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
103 schema:name Biological Sciences
104 rdf:type schema:DefinedTerm
105 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
106 schema:name Biochemistry and Cell Biology
107 rdf:type schema:DefinedTerm
108 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
109 schema:name Genetics
110 rdf:type schema:DefinedTerm
111 sg:journal.1018957 schema:issn 0028-0836
112 1476-4687
113 schema:name Nature
114 schema:publisher Springer Nature
115 rdf:type schema:Periodical
116 sg:person.01023371537.19 schema:affiliation grid-institutes:grid.14709.3b
117 schema:familyName Brisson
118 schema:givenName N.
119 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01023371537.19
120 rdf:type schema:Person
121 sg:person.012070664772.39 schema:familyName Verma
122 schema:givenName D. P. S.
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012070664772.39
124 rdf:type schema:Person
125 sg:person.01367424135.22 schema:affiliation grid-institutes:grid.14709.3b
126 schema:familyName Sullivan
127 schema:givenName D.
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367424135.22
129 rdf:type schema:Person
130 sg:person.07505723122.58 schema:affiliation grid-institutes:grid.24433.32
131 schema:familyName Thomas
132 schema:givenName D. Y.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07505723122.58
134 rdf:type schema:Person
135 sg:pub.10.1038/251074a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048652287
136 https://doi.org/10.1038/251074a0
137 rdf:type schema:CreativeWork
138 sg:pub.10.1038/282471a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004324129
139 https://doi.org/10.1038/282471a0
140 rdf:type schema:CreativeWork
141 grid-institutes:grid.14709.3b schema:alternateName Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada
142 schema:name Department of Biology, McGill University, F3A 2T5, Montreal, Quebec, Canada
143 rdf:type schema:Organization
144 grid-institutes:grid.24433.32 schema:alternateName Department of Molecular Genetics, National Research Council, K1A 0R6, Ottawa, Canada
145 schema:name Department of Molecular Genetics, National Research Council, K1A 0R6, Ottawa, Canada
146 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...