Assembly units of clathrin coats View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-01

AUTHORS

Ernst Ungewickell, Daniel Branton

ABSTRACT

Clathrin, a polypeptide of molecular weight (MW) 180,000, is the main constituent of the polygonal network that forms the coat of coated pits and vesicles; these vesicles play a part in intracellular transport between membranous organelles. This function involves specific recognition of target membranes as well as fusion and fission events that must be coordinated with the assembly, partial disassembly or reorganization of the clathrin coats. To understand these interactions on a molecular level, information about the structure of clathrin and the interactions of clathrin with itself and other proteins is required. Here we show that purified clathrin coats dissociate reversibly into triskelions, structures composed of three usually bent, rather flexible legs irradiated from a centre. We have determined the molecular weight of these triskelions and conclude that they contain trimers of clathrin together with about three light molecular weight polypeptide chains. More... »

PAGES

420-422

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/289420a0

DOI

http://dx.doi.org/10.1038/289420a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040147691

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7464911


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