SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-11

AUTHORS

D. P. Lane, W. K. Hoeffler

ABSTRACT

The large T protein coded by the early region of simian virus 40 (SV40) is involved in the induction and maintenance of cell transformation1. It is not clear which, properties of T are important in causing the transformation, since the protein is multifunctional2–9. To clarify the action of T proteins in transformation, we have prepared several monoclonal antibodies directed against different regions of the T molecule. One of these antibodies, DL 3C4, recognizes an antigenic determinant on T that is also present on a host cell protein of molecular weight 68,000. This cross-reactive 68K protein is located within the nucleus of all mammalian cell types examined and has a characteristic granular distribution as shown by immuno-fluorescence. The antigenic determinant recognized by DL 3C4 is resistant to denaturation. Studies on Adeno-SV40 hybrid viruses show that the antigenic site on T is coded for by sequences located between 0.44 and 0.29 on the SV40 map. More... »

PAGES

167-170

Identifiers

URI

http://scigraph.springernature.com/pub.10.1038/288167a0

DOI

http://dx.doi.org/10.1038/288167a0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007213452

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6159551


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