Human leukocyte interferon produced by E. coli is biologically active View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-10

AUTHORS

David V. Goeddel, Elizabeth Yelverton, Axel Ullrich, Herbert L. Heyneker, Giuseppe Miozzari, William Holmes, Peter H. Seeburg, Thomas Dull, Laurie May, Nowell Stebbing, Roberto Crea, Shuichiro Maeda, Russell McCandliss, Alan Sloma, John M. Tabor, Mitchell Gross, Phillip C. Familletti, Sidney Pestka

ABSTRACT

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection. More... »

PAGES

411-416

Journal

TITLE

Nature

ISSUE

5781

VOLUME

287

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/287411a0

    DOI

    http://dx.doi.org/10.1038/287411a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1049882284

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6159538


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1038/287411a0'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1038/287411a0'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1038/287411a0'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1038/287411a0'


     

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