Expression of human fibroblast interferon gene in Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-09

AUTHORS

Rik Derynck, Erik Remaut, Erik Saman, Patrick Stanssens, Erik De Clercq, Jean Content, Walter Fiers

ABSTRACT

The human fibroblast Interferon gene was inserted in a thermoinducible expression plasmid under control of the phage lambda PL promotor. The primary translation products predicted on the basis of the plasmid constructions were hybrid proteins starting with β-lactamase or phage MS2 polymerase information followed by the total preinterferon. On induction, antiviral activity, whose physico-chemical, immunological and biological characteristics closely corresponded to those of authentic human fibroblast Interferon, was synthesized. Processing to a size compatible with mature but unglycosylated authentic product was observed. More... »

PAGES

193-197

Journal

TITLE

Nature

ISSUE

5779

VOLUME

287

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1038/287193a0

    DOI

    http://dx.doi.org/10.1038/287193a0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1000679818

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6159534


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